Human synaptophysin (SYP) ELISA kit CSB-E17406h CUSABIO
$50.00
In stock
SKU
CSB-E17406h
Product Details
| Target Name | synaptophysin |
| Uniprot No. | P08247 |
| Species | Homo sapiens (Human) |
| Storage | Store at 2-8°C. Please refer to protocol. |
| Sensitivity | 15.6 pg/mL |
| Troubleshooting and FAQs | ELISA kit FAQs |
| Lead Time | 3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx. |
| Associated names | SYP; Synaptophysin; Major synaptic vesicle protein p38 |
| Abbreviation | SYP |
| Sample Types | serum, plasma, tissue homogenates, cell lysates |
| Detection Range | 62.5 pg/mL-4000 pg/mL |
| Assay Time | 1-5h |
| Sample Loading Volume | 50-100ul |
| Detection Wavelength | 450 nm |
| Research Area | Neuroscience |
| Assay Principle | quantitative |
| Measurement | Sandwich |
| Precision | headers: Intra-assay Precision (Precision within an assay): CV%<8%; rows: Intra-assay Precision (Precision within an assay): CV%<8%: Intra-assay Precision (Precision within an assay): CV%<8%; 1: Three samples of known concentration were tested twenty times on one plate to assess.; Intra-assay Precision (Precision within an assay): CV%<8%: Inter-assay Precision (Precision between assays): CV%<10%; 1: Three samples of known concentration were tested in twenty assays to assess.; |
| Linearity | headers: To assess the linearity of the assay, samples were spiked with high concentrations of human SYP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.; rows: To assess the linearity of the assay, samples were spiked with high concentrations of human SYP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.: To assess the linearity of the assay, samples were spiked with high concentrations of human SYP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.; 2: Sample; 3: Serum(n=4); 1: 1:1; 2: Average %; 3: 93; 1: Range %; 2: 88-97; 1: 1:2; 2: Average %; 3: 94; 1: Range %; 2: 87-98; 1: 1:4; 2: Average %; 3: 93; 1: Range %; 2: 88-97; 1: 1:8; 2: Average %; 3: 97; 1: Range %; 2: 92-103 |
| Recovery | headers: The recovery of human SYP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.; rows: The recovery of human SYP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.: The recovery of human SYP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.; 1: Sample Type; 2: Average % Recovery; 3: Range; 1: Serum (n=5); 2: 95; 3: 91-100; 1: EDTA plasma (n=4); 2: 92; 3: 88-96; ; |
| Typical Data | headers: These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.; rows: These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.: These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.; 1: pg/ml OD1 OD2 Average Corrected 4000 2.157 2.258 2.208 2.103 2000 1.776 1.924 1.850 1.745 1000 1.452 1.556 1.504 1.399 500 1.152 1.173 1.163 1.058 250 0.751 0.765 0.758 0.653 125 0.416 0.427 0.422 0.317 62.5 0.262 0.268 0.265 0.160 0 0.104 0.105 0.105; 2: pg/ml; 3: OD1; 4: OD2; 5: Average; 6: Corrected; 8: 4000; 9: 2.157; 10: 2.258; 11: 2.208; 12: 2.103; 14: 2000; 15: 1.776; 16: 1.924; 17: 1.850; 18: 1.745; 20: 1000; 21: 1.452; 22: 1.556; 23: 1.504; 24: 1.399; 26: 500; 27: 1.152; 28: 1.173; 29: 1.163; 30: 1.058; 32: 250; 33: 0.751; 34: 0.765; 35: 0.758; 36: 0.653; 38: 125; 39: 0.416; 40: 0.427; 41: 0.422; 42: 0.317; 44: 62.5; 45: 0.262; 46: 0.268; 47: 0.265; 48: 0.160; 50: 0; 51: 0.104; 52: 0.105; 53: 0.105; 1: pg/ml; 2: OD1; 3: OD2; 4: Average; 5: Corrected; 1: 4000; 2: 2.157; 3: 2.258; 4: 2.208; 5: 2.103; 1: 2000; 2: 1.776; 3: 1.924; 4: 1.850; 5: 1.745; 1: 1000; 2: 1.452; 3: 1.556; 4: 1.504; 5: 1.399; 1: 500; 2: 1.152; 3: 1.173; 4: 1.163; 5: 1.058; 1: 250; 2: 0.751; 3: 0.765; 4: 0.758; 5: 0.653; 1: 125; 2: 0.416; 3: 0.427; 4: 0.422; 5: 0.317; 1: 62.5; 2: 0.262; 3: 0.268; 4: 0.265; 5: 0.160; 1: 0; 2: 0.104; 3: 0.105; 4: 0.105 |
| ELISA Data Analysis | ELISA Standard Curve & Curve Expert software |
| Shelf Life | 6 months |
| Description | Synaptophysin is an integral membrane glycoprotein found in presynaptic vesicles throughout the nervous system. As a member of the synaptophysin family, it plays an important role in synaptic vesicle formation and neurotransmitter release. This protein serves as a widely used marker for synaptic density and neuronal integrity in neuroscience research. Researchers commonly study synaptophysin expression levels in neurodegenerative diseases, synaptic plasticity research, and neuronal development studies. This makes it a valuable biomarker for measuring synaptic function and neuronal health. The Human synaptophysin ELISA kit (CSB-E17406h) uses a quantitative sandwich measurement principle to detect SYP in serum, plasma, tissue homogenates, and cell lysates. The assay offers a detection range of 62.5 pg/mL to 4000 pg/mL with a sensitivity of 15.6 pg/mL. The protocol requires 50-100 μL sample volume and can be completed within 1-5 hours. Detection occurs at 450 nm wavelength, providing researchers with a reliable tool for quantifying human synaptophysin levels in various biological samples. Application Examples Note: The following application examples are drawn from a selection of publications citing this product. For additional applications, please refer to the full list of references in the "Citations" section. This ELISA kit has been used in neurobiological research to quantify synaptophysin levels in extracellular vesicles and neuronally enriched vesicles isolated from human plasma samples. The applications focus on measuring synaptic protein markers as part of broader studies into neuronal function and synaptic integrity. • Extracellular vesicle research: Quantification of synaptophysin in exosomal proteins alongside other synaptic markers to characterize vesicular protein content • Neuronal vesicle studies: Measurement of synaptophysin levels in neuronally enriched extracellular vesicles following immunocapture isolation protocols • Synaptic protein profiling: Analysis of synaptic protein markers in lysed vesicle preparations as part of comprehensive neuronal biomarker panels • Plasma-based biomarker research: Analysis of synaptophysin content in circulating vesicles isolated from participant plasma samples |
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