Mouse Cytochrome P450 2E1(CYP2E1) ELISA kit CSB-EL006425MO CUSABIO
$50.00
In stock
SKU
CSB-EL006425MO
Product Details
| Target Name | cytochrome P450, family 2, subfamily E, polypeptide 1 |
| Uniprot No. | Q05421 |
| Species | Mus musculus (Mouse) |
| Storage | Store at 2-8°C. Please refer to protocol. |
| Sensitivity | 9.75 pg/mL |
| Troubleshooting and FAQs | ELISA kit FAQs |
| Lead Time | 3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx. |
| Associated names | Cyp2e1 ELISA kit; Cyp2e ELISA kit; Cyp2e-1Cytochrome P450 2E1 ELISA kit; EC 1.14.14.1 ELISA kit; 4-nitrophenol 2-hydroxylase ELISA kit; EC 1.14.13.n7 ELISA kit; CYPIIE1 ELISA kit; Cytochrome P450-ALC ELISA kit; Cytochrome P450-J ELISA kit |
| Abbreviation | CYP2E1 |
| Sample Types | serum, plasma, tissue homogenates, cell lysates |
| Detection Range | 39 pg/mL-2500 pg/mL |
| Assay Time | 1-5h |
| Sample Loading Volume | 50-100ul |
| Detection Wavelength | 450 nm |
| Research Area | Cardiovascular |
| Assay Principle | quantitative |
| Measurement | Sandwich |
| Precision | headers: Intra-assay Precision (Precision within an assay): CV%<8%; rows: Intra-assay Precision (Precision within an assay): CV%<8%: Intra-assay Precision (Precision within an assay): CV%<8%; 1: Three samples of known concentration were tested twenty times on one plate to assess.; Intra-assay Precision (Precision within an assay): CV%<8%: Inter-assay Precision (Precision between assays): CV%<10%; 1: Three samples of known concentration were tested in twenty assays to assess.; |
| Linearity | headers: To assess the linearity of the assay, samples were spiked with high concentrations of mouse CYP2E1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.; rows: To assess the linearity of the assay, samples were spiked with high concentrations of mouse CYP2E1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.: To assess the linearity of the assay, samples were spiked with high concentrations of mouse CYP2E1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.; 2: Sample; 3: Serum(n=4); 1: 1:1; 2: Average %; 3: 84; 1: Range %; 2: 80-92; 1: 1:2; 2: Average %; 3: 96; 1: Range %; 2: 92-100; 1: 1:4; 2: Average %; 3: 91; 1: Range %; 2: 85-95; 1: 1:8; 2: Average %; 3: 104; 1: Range %; 2: 100-113 |
| Recovery | headers: The recovery of mouse CYP2E1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.; rows: The recovery of mouse CYP2E1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.: The recovery of mouse CYP2E1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.; ; 1: Sample Type; 2: Average % Recovery; 3: Range; 1: Serum (n=5); 2: 95; 3: 89-100; 1: EDTA plasma (n=4); 2: 92; 3: 88-96; ; |
| Typical Data | headers: These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.; rows: These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.: These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.; ; 1: pg/ml OD1 OD2 Average Corrected 2500 2.482 2.658 2.570 2.384 1250 1.968 2.076 2.022 1.836 625 1.418 1.503 1.461 1.275 312 0.833 0.857 0.845 0.659 156 0.555 0.521 0.538 0.352 78 0.315 0.321 0.318 0.132 39 0.235 0.244 0.240 0.054 0 0.183 0.188 0.186; 2: pg/ml; 3: OD1; 4: OD2; 5: Average; 6: Corrected; 9: 2500; 10: 2.482; 11: 2.658; 12: 2.570; 13: 2.384; 16: 1250; 17: 1.968; 18: 2.076; 19: 2.022; 20: 1.836; 23: 625; 24: 1.418; 25: 1.503; 26: 1.461; 27: 1.275; 30: 312; 31: 0.833; 32: 0.857; 33: 0.845; 34: 0.659; 37: 156; 38: 0.555; 39: 0.521; 40: 0.538; 41: 0.352; 44: 78; 45: 0.315; 46: 0.321; 47: 0.318; 48: 0.132; 51: 39; 52: 0.235; 53: 0.244; 54: 0.240; 55: 0.054; 58: 0; 59: 0.183; 60: 0.188; 61: 0.186; 1: pg/ml; 2: OD1; 3: OD2; 4: Average; 5: Corrected; 1: 2500; 2: 2.482; 3: 2.658; 4: 2.570; 5: 2.384; 1: 1250; 2: 1.968; 3: 2.076; 4: 2.022; 5: 1.836; 1: 625; 2: 1.418; 3: 1.503; 4: 1.461; 5: 1.275; 1: 312; 2: 0.833; 3: 0.857; 4: 0.845; 5: 0.659; 1: 156; 2: 0.555; 3: 0.521; 4: 0.538; 5: 0.352; 1: 78; 2: 0.315; 3: 0.321; 4: 0.318; 5: 0.132; 1: 39; 2: 0.235; 3: 0.244; 4: 0.240; 5: 0.054; 1: 0; 2: 0.183; 3: 0.188; 4: 0.186 |
| ELISA Data Analysis | ELISA Standard Curve & Curve Expert software |
| Shelf Life | 6 months |
| Description | This Mouse CYP2E1 ELISA Kit was designed for the quantitative measurement of Mouse CYP2E1 protein in serum, plasma, tissue homogenates, cell lysates. It is a Sandwich ELISA kit, its detection range is 39 pg/mL-2500 pg/mL and the sensitivity is 9.75 pg/mL. |
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