Mouse Indian Hedgehog (IHH) ELISA kit CSB-E16517m CUSABIO
$50.00
In stock
SKU
CSB-E16517m
Product Details
| Target Name | Indian Hedgehog (IHH) |
| Uniprot No. | P97812 |
| Species | Mus musculus (Mouse) |
| Storage | Store at 2-8°C. Please refer to protocol. |
| Sensitivity | 0.78 pg/mL |
| Troubleshooting and FAQs | ELISA kit FAQs |
| Lead Time | 3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx. |
| Associated names | IhhIndian hedgehog protein ELISA Kit; IHH ELISA Kit; HHG-2) [Cleaved into: Indian hedgehog protein N-product; Indian hedgehog protein C-product] ELISA Kit |
| Abbreviation | IHH |
| Sample Types | serum, plasma, tissue homogenates |
| Detection Range | 3.12 pg/mL-200 pg/mL |
| Assay Time | 1-5h |
| Sample Loading Volume | 50-100ul |
| Detection Wavelength | 450 nm |
| Research Area | Stem Cells |
| Assay Principle | quantitative |
| Measurement | Sandwich |
| Precision | headers: Intra-assay Precision (Precision within an assay): CV%<8%; rows: Intra-assay Precision (Precision within an assay): CV%<8%: Intra-assay Precision (Precision within an assay): CV%<8%; 1: Three samples of known concentration were tested twenty times on one plate to assess.; Intra-assay Precision (Precision within an assay): CV%<8%: Inter-assay Precision (Precision between assays): CV%<10%; 1: Three samples of known concentration were tested in twenty assays to assess.; |
| Linearity | headers: To assess the linearity of the assay, samples were spiked with high concentrations of mouse IHH in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.; rows: To assess the linearity of the assay, samples were spiked with high concentrations of mouse IHH in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.: To assess the linearity of the assay, samples were spiked with high concentrations of mouse IHH in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.; 2: Sample; 3: Serum(n=4); 1: 1:1; 2: Average %; 3: 86; 1: Range %; 2: 82-91; 1: 1:2; 2: Average %; 3: 101; 1: Range %; 2: 98-105; 1: 1:4; 2: Average %; 3: 87; 1: Range %; 2: 84-91; 1: 1:8; 2: Average %; 3: 100; 1: Range %; 2: 95-105 |
| Recovery | headers: The recovery of mouse IHH spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.; rows: The recovery of mouse IHH spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.: The recovery of mouse IHH spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.; ; 1: Sample Type; 2: Average % Recovery; 3: Range; 1: Serum (n=5); 2: 89; 3: 85-94; 1: EDTA plasma (n=4); 2: 107; 3: 103-110; ; |
| Typical Data | headers: These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.; rows: These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.: These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.; ; 1: pg/ml OD1 OD2 Average Corrected 200 1.785 1.831 1.808 1.687 100 1.181 1.201 1.191 1.070 50 0.737 0.800 0.769 0.648 25 0.455 0.435 0.445 0.324 12.5 0.301 0.312 0.307 0.186 6.25 0.208 0.204 0.206 0.085 3.12 0.167 0.161 0.164 0.043 0 0.122 0.120 0.121; 2: pg/ml; 3: OD1; 4: OD2; 5: Average; 6: Corrected; 9: 200; 10: 1.785; 11: 1.831; 12: 1.808; 13: 1.687; 16: 100; 17: 1.181; 18: 1.201; 19: 1.191; 20: 1.070; 23: 50; 24: 0.737; 25: 0.800; 26: 0.769; 27: 0.648; 30: 25; 31: 0.455; 32: 0.435; 33: 0.445; 34: 0.324; 37: 12.5; 38: 0.301; 39: 0.312; 40: 0.307; 41: 0.186; 44: 6.25; 45: 0.208; 46: 0.204; 47: 0.206; 48: 0.085; 51: 3.12; 52: 0.167; 53: 0.161; 54: 0.164; 55: 0.043; 58: 0; 59: 0.122; 60: 0.120; 61: 0.121; 1: pg/ml; 2: OD1; 3: OD2; 4: Average; 5: Corrected; 1: 200; 2: 1.785; 3: 1.831; 4: 1.808; 5: 1.687; 1: 100; 2: 1.181; 3: 1.201; 4: 1.191; 5: 1.070; 1: 50; 2: 0.737; 3: 0.800; 4: 0.769; 5: 0.648; 1: 25; 2: 0.455; 3: 0.435; 4: 0.445; 5: 0.324; 1: 12.5; 2: 0.301; 3: 0.312; 4: 0.307; 5: 0.186; 1: 6.25; 2: 0.208; 3: 0.204; 4: 0.206; 5: 0.085; 1: 3.12; 2: 0.167; 3: 0.161; 4: 0.164; 5: 0.043; 1: 0; 2: 0.122; 3: 0.120; 4: 0.121 |
| ELISA Data Analysis | ELISA Standard Curve & Curve Expert software |
| Shelf Life | 6 months |
| Description | This Mouse IHH ELISA Kit was designed for the quantitative measurement of Mouse IHH protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 3.12 pg/mL-200 pg/mL and the sensitivity is 0.78 pg/mL. |
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