Mouse Interleukin 1β,IL-1β ELISA Kit CSB-E08054m CUSABIO
$50.00
In stock
SKU
CSB-E08054m
Product Details
| Uniprot No. | P10749 |
| Species | Mus musculus (Mouse) |
| Storage | Store at 2-8°C. Please refer to protocol. |
| Sensitivity | 7.8pg/ml |
| Troubleshooting and FAQs | ELISA kit FAQs |
| Lead Time | 3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx. |
| Associated names | Interleukin-1 beta ELISA Kit; IL-1 beta ELISA Kit |
| Abbreviation | IL1B |
| Sample Types | serum, plasma, cell culture supernates, tissuehomogenates |
| Detection Range | 31.25 pg/ml-2000 pg/ml |
| Assay Time | 1-5h |
| Sample Loading Volume | 50-100ul |
| Detection Wavelength | 450 nm |
| Research Area | Immunology |
| Assay Principle | quantitative |
| Measurement | Sandwich |
| Precision | headers: Intra-assay Precision (Precision within an assay): CV%<8%; rows: Intra-assay Precision (Precision within an assay): CV%<8%: Intra-assay Precision (Precision within an assay): CV%<8%; 1: Three samples of known concentration were tested twenty times on one plate to assess.; Intra-assay Precision (Precision within an assay): CV%<8%: Inter-assay Precision (Precision between assays): CV%<10%; 1: Three samples of known concentration were tested in twenty assays to assess. |
| Linearity | headers: To assess the linearity of the assay, samples were spiked with high concentrations of mouse IL-1β in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.; rows: To assess the linearity of the assay, samples were spiked with high concentrations of mouse IL-1β in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.: To assess the linearity of the assay, samples were spiked with high concentrations of mouse IL-1β in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.; ; 2: Sample; 3: Serum(n=4); 1: 1:1; 2: Average %; 3: 88; 2: Range %; 3: 82-95; 1: 1:2; 2: Average %; 3: 93; 2: Range %; 3: 88-98; 1: 1:4; 2: Average %; 3: 96; 2: Range %; 3: 94-102; 1: 1:8; 2: Average %; 3: 94; 2: Range %; 3: 90-100 |
| Recovery | headers: The recovery of mouse IL-1β spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.; rows: The recovery of mouse IL-1β spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.: The recovery of mouse IL-1β spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.; ; 1: Sample Type; 2: Average % Recovery; 3: Range; 1: Serum (n=5); 2: 94; 3: 87-99; 1: EDTA plasma (n=4); 2: 93; 3: 88-98 |
| Typical Data | headers: These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.; rows: These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.: These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.; ; ; 1: pg/ml; 2: OD1; 3: OD2; 4: Average; 5: Corrected; 1: 2000; 2: 2.758; 3: 2.641; 4: 2.700; 5: 2.586; 1: 1000; 2: 1.438; 3: 1.587; 4: 1.513; 5: 1.399; 1: 500; 2: 0.833; 3: 0.869; 4: 0.851; 5: 0.737; 1: 250; 2: 0.474; 3: 0.477; 4: 0.476; 5: 0.362; 1: 125; 2: 0.350; 3: 0.375; 4: 0.363; 5: 0.249; 1: 62.5; 2: 0.267; 3: 0.253; 4: 0.260; 5: 0.146; 1: 31.25; 2: 0.165; 3: 0.186; 4: 0.176; 5: 0.062; 1: 0; 2: 0.119; 3: 0.108; 4: 0.114 |
| Materials provided | A micro ELISA plate --- The 96-well plate has been pre-coated with anti-mouse IL-1β This dismountable microplate can be divided into 12 x 8 strip plates. Two vials lyophilized standard --- Dilute a bottle of the standard at dilution series, read the OD values, and then draw a standard curve. Biotin-labeled IL-1β antibody (100 x concentrate) 1 x 120 μl ---Act as the detection antibody. HRP-avidin (100 x concentrate) 1 x 120 μl --- Bind to the detection antibody and react with the TMB substrate to make the solution chromogenic. Biotin-antibody Diluent 1 x 15 ml ---Dilute the high concentration Biotin-antibody to an appropriate working solution. HRP-avidin Diluent 1 x 15 ml ---Dilute the high concentration HRP-avidin solution to an appropriate solution. Sample Diluent 1 x 50 ml---Dilute the sample to an appropriate concentration. Wash Buffer (25 x concentrate) 1 x 20 ml --- Wash away unbound or free substances. TMB Substrate 1 x 10 ml --- Act as the chromogenic agent. TMB interacts with HRP, eliciting the solution turns blue. Stop Solution 1 x 10 ml --- Stop the color reaction. The solution color immediately turns from blue to yellow. Four Adhesive Strips (For 96 wells) --- Cover the microplate when incubation. An instruction manual |
| Materials not provided | A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 540 nm or 570 nm. An incubator can provide stable incubation conditions up to 37°C±5°C. Centrifuge Vortex Squirt bottle, manifold dispenser, or automated microplate washer Absorbent paper for blotting the microtiter plate 50-300ul multi-channel micropipette Pipette tips Single-channel micropipette with different ranges 100ml and 500ml graduated cylinders Deionized or distilled water Timer Test tubes for dilution |
| ELISA Data Analysis | ELISA Standard Curve & Curve Expert software |
| Shelf Life | 6 months |
| Description | Interleukin-1 beta (IL-1β) is a potent pro-inflammatory cytokine that plays a central role in innate immune responses and inflammatory processes. Activated macrophages, monocytes, and other immune cells produce IL-1β, which mediates fever, acute-phase responses, and tissue inflammation through binding to the IL-1 receptor. This cytokine is essential for host defense against pathogens but can also contribute to pathological inflammation in autoimmune diseases, making it an important target for immunological and inflammatory disease research. The Mouse Interleukin 1β,IL-1β ELISA Kit (CSB-E08054m) provides quantitative measurement of IL-1β in mouse samples using a sandwich assay principle. This kit requires 50-100 μL sample volume and can be completed within 1-5 hours. Detection occurs at 450 nm wavelength, allowing researchers to accurately quantify IL-1β levels in their experimental samples for studies involving Mus musculus. Application Examples Note: The following application examples are drawn from a selection of publications citing this product. For additional applications, please refer to the full list of references in the "Citations" section. This ELISA kit has been used in diverse research applications to quantify interleukin-1 beta levels in mouse models across multiple biological contexts. The kit works well for analyzing various sample types including serum, plasma, bronchoalveolar lavage fluid, and cell culture supernatants from macrophage studies. • Inflammatory cytokine profiling - Measurement of IL-1β alongside other pro-inflammatory mediators in comprehensive cytokine panels to assess inflammatory responses • Respiratory inflammation research - Quantification in bronchoalveolar lavage fluid to evaluate pulmonary inflammatory processes and airway responses • Heat stroke pathophysiology - Measuring IL-1β levels during recovery phases to understand inflammatory mechanisms in thermal injury models • Macrophage activation studies - Analysis of IL-1β secretion from cultured bone marrow-derived macrophages following stimulation protocols • Multi-organ inflammatory studies - Simultaneous evaluation in serum and tissue samples to characterize systemic versus localized inflammatory responses • Biomarker correlation studies - Integration with other inflammatory markers and tissue damage indicators to establish comprehensive inflammatory profiles • Pharmacological intervention research - Evaluation of IL-1β modulation in response to various therapeutic compounds and experimental treatments |
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