Mouse Interleukin 22(IL-22) ELISA Kit CSB-E13513m-IS CUSABIO
$50.00
In stock
SKU
CSB-E13513m-IS
Product Details
| Uniprot No. | Q9JJY9 |
| Species | Mus musculus (Mouse) |
| Storage | Store at 2-8°C. Please refer to protocol. |
| Sensitivity | 3.9 pg/mL |
| Troubleshooting and FAQs | ELISA kit FAQs |
| Lead Time | 3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx. |
| Associated names | Il22 ELISA Kit; Il22a ELISA Kit; Iltif ELISA Kit; IltifaInterleukin-22 ELISA Kit; IL-22 ELISA Kit; IL-10-related T-cell-derived-inducible factor ELISA Kit; IL-TIF ELISA Kit; IL-TIF alpha ELISA Kit; Interleukin-22a ELISA Kit; IL-22a ELISA Kit |
| Abbreviation | IL22 |
| Sample Types | serum, plasma, tissue homogenates, cell culture supernates |
| Detection Range | 15.6 pg/mL-1000 pg/mL |
| Assay Time | 1-5h |
| Sample Loading Volume | 50-100ul |
| Detection Wavelength | 450 nm |
| Research Area | Immunology |
| Assay Principle | quantitative |
| Measurement | Sandwich |
| Precision | headers: Intra-assay Precision (Precision within an assay): CV%<8%; rows: Intra-assay Precision (Precision within an assay): CV%<8%: Intra-assay Precision (Precision within an assay): CV%<8%; 1: Three samples of known concentration were tested twenty times on one plate to assess.; Intra-assay Precision (Precision within an assay): CV%<8%: Inter-assay Precision (Precision between assays): CV%<10%; 1: Three samples of known concentration were tested in twenty assays to assess. |
| Linearity | headers: To assess the linearity of the assay, samples were spiked with high concentrations of mouse IL-22 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.; rows: To assess the linearity of the assay, samples were spiked with high concentrations of mouse IL-22 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.: To assess the linearity of the assay, samples were spiked with high concentrations of mouse IL-22 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.; 2: Sample; 3: Serum(n=4); 1: 1:1; 2: Average %; 3: 89; 1: Range %; 2: 82-96; 1: 1:2; 2: Average %; 3: 94; 1: Range %; 2: 89-100; 1: 1:4; 2: Average %; 3: 99; 1: Range %; 2: 94-105; 1: 1:8; 2: Average %; 3: 93; 1: Range %; 2: 86-100 |
| Recovery | headers: The recovery of mouse IL-22 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.; rows: The recovery of mouse IL-22 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.: The recovery of mouse IL-22 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.; 1: Sample Type; 2: Average % Recovery; 3: Range; 1: Serum (n=5); 2: 92; 3: 87-98; 1: EDTA plasma (n=4); 2: 97; 3: 90-106 |
| Typical Data | headers: These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.; rows: These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.: These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.; ; ; 1: pg/ml; 2: OD1; 3: OD2; 4: Average; 5: Corrected; 1: 1000; 2: 2.972; 3: 2.834; 4: 2.903; 5: 2.721; 1: 500; 2: 2.406; 3: 2.367; 4: 2.387; 5: 2.205; 1: 250; 2: 1.525; 3: 1.538; 4: 1.531; 5: 1.350; 1: 125; 2: 0.914; 3: 0.929; 4: 0.921; 5: 0.740; 1: 62.5; 2: 0.565; 3: 0.551; 4: 0.558; 5: 0.377; 1: 31.2; 2: 0.373; 3: 0.353; 4: 0.363; 5: 0.182; 1: 15.6; 2: 0.277; 3: 0.289; 4: 0.283; 5: 0.102; 1: 0; 2: 0.183; 3: 0.180; 4: 0.182 |
| ELISA Data Analysis | ELISA Standard Curve & Curve Expert software |
| Shelf Life | 6 months |
| Description | This kit quantitatively measures interleukin-22 (IL-22) in mouse serum, plasma, tissue homogenates, and cell culture supernates, supporting immunological research into mucosal barrier defense and tissue homeostasis. Because IL-22 is produced primarily by Th17 cells, ILC3s, and γδ T cells and acts locally at epithelial surfaces, researchers often need to detect it across both systemic and tissue-level compartments within a single study. Detection Range: The 15.6–1000 pg/mL range captures IL-22 concentrations typically observed in mouse serum during colitis, dermatitis, or infection models, where levels commonly reach tens to hundreds of pg/mL. Most inflamed tissue homogenates and stimulated splenocyte supernates fall within this window without requiring extensive dilution, though samples from acute DSS colitis models at peak inflammation may need dilution to stay on-curve. Sensitivity: At 3.9 pg/mL, the kit detects IL-22 in naïve or mildly stimulated conditions where circulating levels are near-baseline — critical for establishing pre-disease time points or measuring output from low-frequency ILC3 populations in sorted cell cultures. Sample Compatibility: Validated use across plasma, serum, tissue homogenates, and culture supernates allows researchers to correlate systemic IL-22 levels with local production in gut or skin tissue within the same experiment, while also linking findings to in vitro polarization assays. Assay Efficiency: The 1–5 hour protocol using 50–100 µL sample volumes accommodates studies involving serial bleeds from individual mice and limited yields from lamina propria lymphocyte isolations, preserving material for parallel cytokine measurements. This kit is directly applicable to studies of inflammatory bowel disease, psoriasis-like dermatitis, and host defense against enteric bacterial pathogens such as *Citrobacter rodentium*. |
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