Mouse Interleukin 33(IL-33) ELISA Kit CSB-E14393m CUSABIO
$50.00
In stock
SKU
CSB-E14393m
Product Details
| Uniprot No. | Q8BVZ5 |
| Species | Mus musculus (Mouse) |
| Storage | Store at 2-8°C. Please refer to protocol. |
| Sensitivity | 3.9 pg/ml |
| Troubleshooting and FAQs | ELISA kit FAQs |
| Lead Time | 3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx. |
| Associated names | Il33Interleukin-33 ELISA Kit; IL-33) [Cleaved into: Interleukin-33(102-266); Interleukin-33(109-266)] ELISA Kit |
| Abbreviation | IL33 |
| Sample Types | serum, plasma, tissue homogenates |
| Detection Range | 15.6 pg/ml - 1000 pg/ml |
| Assay Time | 1-5h |
| Sample Loading Volume | 50-100ul |
| Detection Wavelength | 450 nm |
| Research Area | Immunology |
| Assay Principle | quantitative |
| Measurement | Sandwich |
| Precision | headers: Intra-assay Precision (Precision within an assay): CV%<8%; rows: Intra-assay Precision (Precision within an assay): CV%<8%: Intra-assay Precision (Precision within an assay): CV%<8%; 1: Three samples of known concentration were tested twenty times on one plate to assess.; Intra-assay Precision (Precision within an assay): CV%<8%: Inter-assay Precision (Precision between assays): CV%<10%; 1: Three samples of known concentration were tested in twenty assays to assess. |
| Linearity | headers: To assess the linearity of the assay, samples were spiked with high concentrations of mouse IL-33 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.; rows: To assess the linearity of the assay, samples were spiked with high concentrations of mouse IL-33 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.: To assess the linearity of the assay, samples were spiked with high concentrations of mouse IL-33 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.; 2: Sample; 3: Serum(n=4); 1: 1:1; 2: Average %; 3: 100; 1: Range %; 2: 93-105; 1: 1:2; 2: Average %; 3: 97; 1: Range %; 2: 92-103; 1: 1:4; 2: Average %; 3: 104; 1: Range %; 2: 97-111; 1: 1:8; 2: Average %; 3: 100; 1: Range %; 2: 93-105 |
| Recovery | headers: The recovery of mouse IL-33 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.; rows: The recovery of mouse IL-33 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.: The recovery of mouse IL-33 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.; 1: Sample Type; 2: Average % Recovery; 3: Range; 1: Serum (n=5); 2: 98; 3: 93-103; 1: EDTA plasma (n=4); 2: 93; 3: 89-98 |
| Typical Data | headers: These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.; rows: These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.: These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.; ; ; 1: pg/ml; 2: OD1; 3: OD2; 4: Average; 5: Corrected; 1: 1000; 2: 2.963; 3: 2.834; 4: 2.899; 5: 2.721; 1: 500; 2: 2.218; 3: 2.167; 4: 2.192; 5: 2.015; 1: 250; 2: 1.700; 3: 1.658; 4: 1.679; 5: 1.502; 1: 125; 2: 1.184; 3: 1.159; 4: 1.172; 5: 0.994; 1: 62.5; 2: 0.648; 3: 0.671; 4: 0.659; 5: 0.482; 1: 31.2; 2: 0.404; 3: 0.413; 4: 0.408; 5: 0.231; 1: 15.6; 2: 0.289; 3: 0.284; 4: 0.286; 5: 0.109; 1: 0; 2: 0.175; 3: 0.180; 4: 0.177 |
| ELISA Data Analysis | ELISA Standard Curve & Curve Expert software |
| Shelf Life | 6 months |
| Description | This Mouse IL33 ELISA Kit was designed for the quantitative measurement of Mouse IL33 protein in serum, plasma, cell culture supernates, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 31.25 pg/mL-2000 pg/mL and the sensitivity is 7.8 pg/mL. |
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