Mouse Super Oxidase Dimutase,SOD ELISA Kit CSB-E08556m CUSABIO
$50.00
In stock
SKU
CSB-E08556m
Product Details
| Target Name | superoxide dismutase 1, soluble |
| Uniprot No. | P08228 |
| Species | Mus musculus (Mouse) |
| Storage | Store at 2-8°C. Please refer to protocol. |
| Sensitivity | 5.4 pg/mL |
| Troubleshooting and FAQs | ELISA kit FAQs |
| Lead Time | 3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx. |
| Associated names | Sod1 ELISA Kit; Superoxide dismutase [Cu-Zn] ELISA Kit; EC 1.15.1.1 ELISA Kit |
| Abbreviation | SOD1 |
| Sample Types | serum, plasma, tissue homogenates |
| Detection Range | 5.4 pg/mL-4000 pg/mL |
| Assay Time | 1-5h |
| Sample Loading Volume | 50-100ul |
| Detection Wavelength | 450 nm |
| Research Area | Metabolism |
| Precision | headers: Intra-assay Precision (Precision within an assay): CV%<8%; rows: Intra-assay Precision (Precision within an assay): CV%<8%: Intra-assay Precision (Precision within an assay): CV%<8%; 1: Three samples of known concentration were tested twenty times on one plate to assess.; Intra-assay Precision (Precision within an assay): CV%<8%: Inter-assay Precision (Precision between assays): CV%<10%; 1: Three samples of known concentration were tested in twenty assays to assess. |
| Linearity | headers: To assess the linearity of the assay, samples were spiked with high concentrations of mouse SOD in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.; rows: To assess the linearity of the assay, samples were spiked with high concentrations of mouse SOD in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.: To assess the linearity of the assay, samples were spiked with high concentrations of mouse SOD in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.; 2: Sample; 3: Serum(n=4); 1: 1:200; 2: Average %; 3: 96; 1: Range %; 2: 85-99; 1: 1:400; 2: Average %; 3: 95; 1: Range %; 2: 91-101; 1: 1:800; 2: Average %; 3: 96; 1: Range %; 2: 92-100; 1: 1:1600; 2: Average %; 3: 93; 1: Range %; 2: 84-98 |
| Recovery | headers: The recovery of human LIF spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.; rows: The recovery of human LIF spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.: The recovery of human LIF spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.; 1: Sample Type; 2: Average % Recovery; 3: Range; 1: Serum (n=5); 2: 95; 3: 89-101; 1: EDTA plasma (n=4); 2: 94; 3: 90-100 |
| Typical Data | headers: These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.; rows: These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.: These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.; ; ; 1: pg/ml; 2: OD1; 3: OD2; 4: Average; 5: Corrected; 1: 4000; 2: 2.312; 3: 2.434; 4: 2.373; 5: 2.271; 1: 1333.3; 2: 1.694; 3: 1.667; 4: 1.681; 5: 1.579; 1: 444.4; 2: 1.027; 3: 1.020; 4: 1.023; 5: 0.922; 1: 148.1; 2: 0.526; 3: 0.509; 4: 0.518; 5: 0.416; 1: 49.3; 2: 0.294; 3: 0.311; 4: 0.303; 5: 0.201; 1: 16.4; 2: 0.183; 3: 0.193; 4: 0.188; 5: 0.086; 1: 5.4; 2: 0.135; 3: 0.141; 4: 0.138; 5: 0.036; 1: 0; 2: 0.098; 3: 0.105; 4: 0.102 |
| Materials provided | A micro ELISA plate ---The 96-well plate has been pre-coated with an anti-mouse SOD antibody. This dismountable microplate can be divided into 12 x 8 strip plates. Two vials lyophilized standard ---Dilute a bottle of the standard at dilution series, read the OD values, and then draw a standard curve. One vial Biotin-labeled SOD antibody (100 x concentrate) (120 μl/bottle) ---Act as the detection antibody. One vial HRP-avidin (100 x concentrate) (120 μl/bottle) ---Bind to the detection antibody and react with the TMB substrate to make the solution chromogenic. One vial Biotin-antibody Diluent (15 ml/bottle) ---Dilute the Biotin-antibody. One vial HRP-avidin Diluent (15 ml/bottle) ---Dilute the HRP-avidin solution. One vial Sample Diluent (50 ml/bottle)---Dilute the sample to an appropriate concentration. One vial Wash Buffer (25 x concentrate) (20 ml/bottle) ---Wash away unbound or free substances. One vial TMB Substrate (10 ml/bottle) ---Act as the chromogenic agent. TMB interacts with HRP, eliciting the solution turns blue. One vial Stop Solution (10 ml/bottle) ---Stop the color reaction. The solution color immediately turns from blue to yellow. Four Adhesive Strips (For 96 wells) --- Cover the microplate when incubation. An instruction manual |
| Materials not provided | A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 540 nm or 570 nm. An incubator can provide stable incubation conditions up to 37°C±5°C. Centrifuge Vortex Squirt bottle, manifold dispenser, or automated microplate washer Absorbent paper for blotting the microtiter plate 50-300ul multi-channel micropipette Pipette tips Single-channel micropipette with different ranges 100ml and 500ml graduated cylinders Deionized or distilled water Timer Test tubes for dilution |
| Shelf Life | 6 months |
| Description | The Mouse Super Oxidase Dimutase (SOD) ELISA Kit allows for the detection and quantification of SOD in mouse serum, plasma, or tissue homogenates. This kit has high sensitivity, excellent specificity, precision less than 10%, and consistency between batches. It employs the sandwich ELISA mechanism and enzyme-substrate chromogenic reaction to achieve the detection. The solution color develops in proportion to the amount of SOD in the sample, and the color intensity can be measured at 450 nm via a microplate reader. SOD is a homo-dimeric metalloprotein that catalyzes the dismutation of the free superoxide (O2−) radical into molecular oxygen (O2) and hydrogen peroxide (H2O2). It primarily localizes to the cytoplasm but is also found in the nucleus and the intermembrane space (IMS) of the mitochondria. SOD is an important antioxidant defense in nearly all living cells exposed to oxygen. The dismutase activity of SOD may be essential to the accumulation of reactive oxygen species (ROS) and misfolded proteins in the IMS of the mitochondria. Additionally, heterozygous variants in SOD are a common cause of familial amyotrophic lateral sclerosis (ALS), a form of motor neuron disease. |
MDSD
Reviews
Write Your Own Review
Related Products
Check items to add to the cart or
