Mouse interferon-inducible protein 10,IP-10 ELISA Kit CSB-E08183m-IS CUSABIO
$50.00
In stock
SKU
CSB-E08183m-IS
Product Details
| Uniprot No. | P17515 |
| Species | Mus musculus (Mouse) |
| Storage | Store at 2-8°C. Please refer to protocol. |
| Sensitivity | 3.9 pg/mL |
| Troubleshooting and FAQs | ELISA kit FAQs |
| Associated names | Cxcl10 ELISA kit; Crg2 ELISA kit; Ifi10 ELISA kit; Inp10 ELISA kit; Scyb10C-X-C motif chemokine 10 ELISA kit; 10 kDa interferon gamma-induced protein ELISA kit; Gamma-IP10 ELISA kit; IP-10 ELISA kit; C7 ELISA kit; Interferon-gamma induced protein CRG-2 ELISA kit; Small-inducible cytokine B10 ELISA kit |
| Abbreviation | CXCL10 |
| Sample Types | serum, plasma, tissue homogenates |
| Detection Range | 7.8 pg/mL-500 pg/mL |
| Assay Time | 1-5h |
| Sample Loading Volume | 50-100ul |
| Detection Wavelength | 450 nm |
| Research Area | Immunology |
| Assay Principle | quantitative |
| Measurement | Sandwich |
| Precision | headers: Intra-assay Precision (Precision within an assay): CV%<8%; rows: Intra-assay Precision (Precision within an assay): CV%<8%: Intra-assay Precision (Precision within an assay): CV%<8%; 1: Three samples of known concentration were tested twenty times on one plate to assess.; Intra-assay Precision (Precision within an assay): CV%<8%: Inter-assay Precision (Precision between assays): CV%<10%; 1: Three samples of known concentration were tested in twenty assays to assess. |
| Linearity | headers: To assess the linearity of the assay, samples were spiked with high concentrations of mouse IP-10 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.; rows: To assess the linearity of the assay, samples were spiked with high concentrations of mouse IP-10 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.: To assess the linearity of the assay, samples were spiked with high concentrations of mouse IP-10 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.; 2: Sample; 3: Serum(n=4); 1: 1:4; 2: Average %; 3: 98; 1: Range %; 2: 91-104; 1: 1:8; 2: Average %; 3: 94; 1: Range %; 2: 89-99; 1: 1:16; 2: Average %; 3: 105; 1: Range %; 2: 100-111; 1: 1:32; 2: Average %; 3: 90; 1: Range %; 2: 85-96 |
| Recovery | headers: The recovery of mouse IP-10 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.; rows: The recovery of mouse IP-10 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.: The recovery of mouse IP-10 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.; 1: Sample Type; 2: Average % Recovery; 3: Range; 1: Serum (n=5); 2: 98; 3: 91-105; 1: EDTA plasma (n=4); 2: 90; 3: 85-96 |
| Typical Data | headers: These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.; rows: These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.: These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.; ; ; 1: pg/ml; 2: OD1; 3: OD2; 4: Average; 5: Corrected; 1: 500; 2: 3.083; 3: 2.934; 4: 3.009; 5: 2.822; 1: 250; 2: 1.954; 3: 1.867; 4: 1.911; 5: 1.723; 1: 125; 2: 1.267; 3: 1.228; 4: 1.248; 5: 1.060; 1: 62.5; 2: 0.815; 3: 0.829; 4: 0.822; 5: 0.635; 1: 31.2; 2: 0.482; 3: 0.471; 4: 0.477; 5: 0.289; 1: 15.6; 2: 0.369; 3: 0.353; 4: 0.361; 5: 0.174; 1: 7.8; 2: 0.304; 3: 0.304; 4: 0.304; 5: 0.117; 1: 0; 2: 0.189; 3: 0.185; 4: 0.187 |
| ELISA Data Analysis | ELISA Standard Curve & Curve Expert software |
| Shelf Life | 6 months |
| Description | This Mouse CXCL10 (IP-10) ELISA Kit provides quantitative measurement of CXCL10 in Mus musculus samples, supporting research into interferon-γ-driven immune signaling and chemokine-mediated T cell recruitment. Because CXCL10 levels can shift dramatically between baseline and inflammatory states, the kit is designed to capture this dynamic range without requiring multiple dilution schemes. Detection Range: The 7.8–500 pg/mL range spans resting-state serum CXCL10 concentrations in naïve mice (typically 20–80 pg/mL) through the elevated levels seen during viral infection or tumor-driven inflammation (often 200–500+ pg/mL), meaning most samples can be run neat or with minimal dilution. Sensitivity: At 3.9 pg/mL, the kit detects CXCL10 in low-abundance contexts such as conditioned media from unstimulated splenocytes or early-phase innate immune responses before IFN-γ amplification drives robust chemokine production. Sample Compatibility: Validation across serum, plasma, and tissue homogenates enables researchers to correlate circulating CXCL10 with local expression in inflamed tissues—critical for dissecting compartmentalized immune responses in organs like lung, liver, or tumor microenvironment. Assay Efficiency: The 50–100 µL sample volume and 1–5 hour protocol accommodate limited-volume mouse bleeds and same-day processing of tissue panels from multi-timepoint infection or treatment studies. This kit is well-suited for studies of viral immunopathology, graft rejection, and CXCR3-dependent antitumor immunity. |
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