Rat 3-oxo-5-alpha-steroid 4-dehydrogenase 2(SRD5A2) ELISA kit CSB-EL022654RA CUSABIO
$50.00
In stock
SKU
CSB-EL022654RA
Product Details
| Target Name | steroid-5-alpha-reductase, alpha polypeptide 2 (3-oxo-5 alpha-steroid delta 4-dehydrogenase alpha 2) |
| Uniprot No. | P31214 |
| Species | Rattus norvegicus (Rat) |
| Storage | Store at 2-8°C. Please refer to protocol. |
| Sensitivity | 3 pg/mL |
| Troubleshooting and FAQs | ELISA kit FAQs |
| Lead Time | 3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx. |
| Associated names | Srd5a2; 3-oxo-5-alpha-steroid 4-dehydrogenase 2; 5 alpha-SR2; SR type 2; Steroid 5-alpha-reductase 2; S5AR 2 |
| Abbreviation | SRD5A2 |
| Sample Types | serum, plasma, tissue homogenates, cell lysates |
| Detection Range | 6 pg/mL-1200 pg/mL |
| Assay Time | 1-5h |
| Sample Loading Volume | 50-100ul |
| Detection Wavelength | 450 nm |
| Research Area | Signal Transduction |
| Assay Principle | quantitative |
| Measurement | Competitive |
| Precision | headers: Intra-assay Precision (Precision within an assay): CV%<15%; rows: Intra-assay Precision (Precision within an assay): CV%<15%: Intra-assay Precision (Precision within an assay): CV%<15%; 1: Three samples of known concentration were tested twenty times on one plate to assess.; Intra-assay Precision (Precision within an assay): CV%<15%: Inter-assay Precision (Precision between assays): CV%<15%; 1: Three samples of known concentration were tested in twenty assays to assess.; |
| Linearity | headers: To assess the linearity of the assay, samples were spiked with high concentrations of rat SRD5A2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.; rows: To assess the linearity of the assay, samples were spiked with high concentrations of rat SRD5A2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.: To assess the linearity of the assay, samples were spiked with high concentrations of rat SRD5A2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.; 2: Sample; 3: Serum(n=4); 1: 1:1; 2: Average %; 3: 92; 1: Range %; 2: 88-96; 1: 1:2; 2: Average %; 3: 94; 1: Range %; 2: 89-99; 1: 1:4; 2: Average %; 3: 96; 1: Range %; 2: 90-103; 1: 1:8; 2: Average %; 3: 90; 1: Range %; 2: 87-94 |
| Recovery | headers: The recovery of rat SRD5A2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.; rows: The recovery of rat SRD5A2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.: The recovery of rat SRD5A2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.; 1: Sample Type; 2: Average % Recovery; 3: Range; 1: Serum (n=5); 2: 98; 3: 95-101; 1: EDTA plasma (n=4); 2: 93; 3: 87-100; ; |
| Typical Data | headers: These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.; rows: These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.: These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.; 1: pg/ml OD1 OD2 Average 1200 0.225 0.217 0.221 300 0.411 0.434 0.423 96 0.651 0.632 0.642 24 1.055 1.017 1.036 6 1.497 1.458 1.478 0 2.359 2.450 2.405; 2: pg/ml; 3: OD1; 4: OD2; 5: Average; 8: 1200; 9: 0.225; 10: 0.217; 11: 0.221; 14: 300; 15: 0.411; 16: 0.434; 17: 0.423; 20: 96; 21: 0.651; 22: 0.632; 23: 0.642; 26: 24; 27: 1.055; 28: 1.017; 29: 1.036; 32: 6; 33: 1.497; 34: 1.458; 35: 1.478; 38: 0; 39: 2.359; 40: 2.450; 41: 2.405; 1: pg/ml; 2: OD1; 3: OD2; 4: Average; 1: 1200; 2: 0.225; 3: 0.217; 4: 0.221; 1: 300; 2: 0.411; 3: 0.434; 4: 0.423; 1: 96; 2: 0.651; 3: 0.632; 4: 0.642; 1: 24; 2: 1.055; 3: 1.017; 4: 1.036; 1: 6; 2: 1.497; 3: 1.458; 4: 1.478; 1: 0; 2: 2.359; 3: 2.450; 4: 2.405 |
| ELISA Data Analysis | ELISA Standard Curve & Curve Expert software |
| Shelf Life | 6 months |
| Description | The Rat 3-oxo-5-alpha-steroid 4-dehydrogenase 2 (SRD5A2) ELISA kit is the ultimate tool for researchers seeking to quantify SRD5A2 levels in samples of rat serum, plasma, tissue homogenates, and cell lysates. The SRD5A2 protein, also known as 3-oxo-5-alpha-steroid 4-dehydrogenase 2, is involved in processing androgens, which are hormones that direct male sexual development,making it an essential protein for researchers in this field. Our ELISA kit is designed to provide fast, accurate, and reliable results, allowing researchers to measure SRD5A2 levels with high sensitivity and precision. With a detection range of 6 pg/mL to 1200 pg/mL and a sensitivity of 3 pg/mL, this ELISA kit delivers precise and accurate results. The assay time is just 1-5 hours, and the sample volume required is only 50-100ul, making it a highly efficient tool for researchers. The kit uses a competitive assay principle, enabling quantitative measurement of SRD5A2 levels in samples. The detection wavelength is 450 nm, making it easy to read and interpret results. |
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