Rat Epithelial-Cadherin,E-Cad ELISA Kit CSB-E07308r CUSABIO
$50.00
In stock
SKU
CSB-E07308r
Product Details
| Target Name | cadherin 1, type 1, E-cadherin (epithelial) |
| Uniprot No. | Q9R0T4 |
| Species | Rattus norvegicus (Rat) |
| Storage | Store at 2-8°C. Please refer to protocol. |
| Sensitivity | 0.78 ng/mL |
| Troubleshooting and FAQs | ELISA kit FAQs |
| Lead Time | 3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx. |
| Associated names | Cdh1Cadherin-1 ELISA kit; Epithelial cadherin ELISA kit; E-cadherin ELISA kit; Uvomorulin ELISA kit; CD antigen CD324) [Cleaved into: E-Cad/CTF1; E-Cad/CTF2; E-Cad/CTF3] ELISA kit |
| Abbreviation | CDH1 |
| Sample Types | serum, plasma, tissue homogenates, cell lysates |
| Detection Range | 3.12 ng/mL-200 ng/mL |
| Assay Time | 1-5h |
| Sample Loading Volume | 50-100ul |
| Detection Wavelength | 450 nm |
| Research Area | Cancer |
| Assay Principle | quantitative |
| Measurement | Sandwich |
| Precision | headers: Intra-assay Precision (Precision within an assay): CV%<8%; rows: Intra-assay Precision (Precision within an assay): CV%<8%: Intra-assay Precision (Precision within an assay): CV%<8%; 1: Three samples of known concentration were tested twenty times on one plate to assess.; Intra-assay Precision (Precision within an assay): CV%<8%: Inter-assay Precision (Precision between assays): CV%<10%; 1: Three samples of known concentration were tested in twenty assays to assess.; |
| Linearity | headers: To assess the linearity of the assay, samples were spiked with high concentrations of rat E-Cad in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.; rows: To assess the linearity of the assay, samples were spiked with high concentrations of rat E-Cad in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.: To assess the linearity of the assay, samples were spiked with high concentrations of rat E-Cad in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.; 2: Sample; 3: Serum(n=4); 1: 1:1; 2: Average %; 3: 87; 1: Range %; 2: 85-90; 1: 1:2; 2: Average %; 3: 102; 1: Range %; 2: 98-106; 1: 1:4; 2: Average %; 3: 99; 1: Range %; 2: 95-103; 1: 1:8; 2: Average %; 3: 83; 1: Range %; 2: 80-86 |
| Recovery | headers: The recovery of rat E-Cad spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.; rows: The recovery of rat E-Cad spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.: The recovery of rat E-Cad spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.; ; 1: Sample Type; 2: Average % Recovery; 3: Range; 1: Serum (n=5); 2: 105; 3: 101-109; 1: EDTA plasma (n=4); 2: 97; 3: 93-100; ; |
| Typical Data | headers: These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.; rows: These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.: These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.; ; 1: ng/ml OD1 OD2 Average Corrected 200 2.750 2.798 2.774 2.591 100 2.045 1.968 2.007 1.824 50 1.401 1.358 1.380 1.197 25 0.920 0.967 0.944 0.761 12.5 0.576 0.598 0.587 0.404 6.25 0.445 0.468 0.457 0.274 3.12 0.307 0.312 0.310 0.127 0 0.182 0.183 0.183; 2: ng/ml; 3: OD1; 4: OD2; 5: Average; 6: Corrected; 9: 200; 10: 2.750; 11: 2.798; 12: 2.774; 13: 2.591; 16: 100; 17: 2.045; 18: 1.968; 19: 2.007; 20: 1.824; 23: 50; 24: 1.401; 25: 1.358; 26: 1.380; 27: 1.197; 30: 25; 31: 0.920; 32: 0.967; 33: 0.944; 34: 0.761; 37: 12.5; 38: 0.576; 39: 0.598; 40: 0.587; 41: 0.404; 44: 6.25; 45: 0.445; 46: 0.468; 47: 0.457; 48: 0.274; 51: 3.12; 52: 0.307; 53: 0.312; 54: 0.310; 55: 0.127; 58: 0; 59: 0.182; 60: 0.183; 61: 0.183; 1: ng/ml; 2: OD1; 3: OD2; 4: Average; 5: Corrected; 1: 200; 2: 2.750; 3: 2.798; 4: 2.774; 5: 2.591; 1: 100; 2: 2.045; 3: 1.968; 4: 2.007; 5: 1.824; 1: 50; 2: 1.401; 3: 1.358; 4: 1.380; 5: 1.197; 1: 25; 2: 0.920; 3: 0.967; 4: 0.944; 5: 0.761; 1: 12.5; 2: 0.576; 3: 0.598; 4: 0.587; 5: 0.404; 1: 6.25; 2: 0.445; 3: 0.468; 4: 0.457; 5: 0.274; 1: 3.12; 2: 0.307; 3: 0.312; 4: 0.310; 5: 0.127; 1: 0; 2: 0.182; 3: 0.183; 4: 0.183 |
| ELISA Data Analysis | ELISA Standard Curve & Curve Expert software |
| Shelf Life | 6 months |
| Description | This Rat CDH1 ELISA Kit was designed for the quantitative measurement of Rat CDH1 protein in serum, plasma, tissue homogenates, cell lysates. It is a Sandwich ELISA kit, its detection range is 3.12 ng/mL-200 ng/mL and the sensitivity is 0.78 ng/mL. |
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