Sheep Interleukin 10,IL-10 ELISA Kit CSB-E12817Sh CUSABIO
$50.00
In stock
SKU
CSB-E12817Sh
Product Details
| Target Name | interleukin 10 |
| Uniprot No. | Q29408 |
| Species | Ovis aries (Sheep) |
| Storage | Store at 2-8°C. Please refer to protocol. |
| Sensitivity | 5 pg/mL |
| Troubleshooting and FAQs | ELISA kit FAQs |
| Lead Time | 3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx. |
| Associated names | IL10Interleukin-10 ELISA Kit; IL-10 ELISA Kit; Cytokine synthesis inhibitory factor ELISA Kit; CSIF ELISA Kit |
| Abbreviation | IL10 |
| Sample Types | serum, plasma, tissue homogenates |
| Detection Range | 5 pg/mL-80 pg/mL |
| Assay Time | 1-5h |
| Sample Loading Volume | 50-100ul |
| Detection Wavelength | 450 nm |
| Research Area | Immunology |
| Assay Principle | quantitative |
| Measurement | Competitive |
| Precision | headers: Intra-assay Precision (Precision within an assay): CV%<15%; rows: Intra-assay Precision (Precision within an assay): CV%<15%: Intra-assay Precision (Precision within an assay): CV%<15%; 1: Three samples of known concentration were tested twenty times on one plate to assess.; Intra-assay Precision (Precision within an assay): CV%<15%: Inter-assay Precision (Precision between assays): CV%<15%; 1: Three samples of known concentration were tested in twenty assays to assess.; |
| Linearity | headers: To assess the linearity of the assay, samples were spiked with high concentrations of sheep IL-10 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.; rows: To assess the linearity of the assay, samples were spiked with high concentrations of sheep IL-10 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.: To assess the linearity of the assay, samples were spiked with high concentrations of sheep IL-10 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.; 2: Sample; 3: Serum(n=4); 1: 1:1; 2: Average %; 3: 92; 1: Range %; 2: 87-98; 1: 1:2; 2: Average %; 3: 98; 1: Range %; 2: 93-104; 1: 1:4; 2: Average %; 3: 84; 1: Range %; 2: 82-88; 1: 1:8; 2: Average %; 3: 90; 1: Range %; 2: 85-97 |
| Recovery | headers: The recovery of sheep IL-10 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.; rows: The recovery of sheep IL-10 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.: The recovery of sheep IL-10 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.; 1: Sample Type; 2: Average % Recovery; 3: Range; 1: Serum (n=5); 2: 95; 3: 91-99; 1: EDTA plasma (n=4); 2: 92; 3: 87-97; ; |
| Typical Data | headers: These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.; rows: These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.: These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.; 1: pg/ml OD1 OD2 Average 80 0.202 0.196 0.199 40 0.365 0.345 0.355 20 0.623 0.641 0.632 10 1.147 1.168 1.158 5 2.344 2.189 2.267; 2: pg/ml; 3: OD1; 4: OD2; 5: Average; 8: 80; 9: 0.202; 10: 0.196; 11: 0.199; 14: 40; 15: 0.365; 16: 0.345; 17: 0.355; 20: 20; 21: 0.623; 22: 0.641; 23: 0.632; 26: 10; 27: 1.147; 28: 1.168; 29: 1.158; 32: 5; 33: 2.344; 34: 2.189; 35: 2.267; 1: pg/ml; 2: OD1; 3: OD2; 4: Average; 1: 80; 2: 0.202; 3: 0.196; 4: 0.199; 1: 40; 2: 0.365; 3: 0.345; 4: 0.355; 1: 20; 2: 0.623; 3: 0.641; 4: 0.632; 1: 10; 2: 1.147; 3: 1.168; 4: 1.158; 1: 5; 2: 2.344; 3: 2.189; 4: 2.267 |
| ELISA Data Analysis | ELISA Standard Curve & Curve Expert software |
| Shelf Life | 6 months |
| Description | Interleukin 10 (IL-10) is a potent anti-inflammatory cytokine that plays an important role in immune regulation and homeostasis. Regulatory T cells, macrophages, and B cells produce IL-10 primarily. IL-10 works to suppress inflammatory responses by blocking pro-inflammatory cytokine production and reducing antigen presentation. This immunosuppressive activity makes IL-10 necessary for preventing excessive immune activation. It also helps maintain tissue integrity during inflammatory processes. Research into IL-10 levels offers valuable insights into immune system function, inflammatory disease progression, and therapeutic monitoring in various pathological conditions. The Sheep Interleukin 10 ELISA Kit (CSB-E12817Sh) measures IL-10 quantitatively in sheep samples using a competitive assay principle. This kit works with serum, plasma, and tissue homogenates. The detection range spans 5 pg/mL to 80 pg/mL with a sensitivity of 5 pg/mL. The assay needs 50-100 μL sample volume and takes 1-5 hours to complete. Detection occurs at 450 nm wavelength, giving researchers a reliable tool for IL-10 quantification in Ovis aries specimens. Application Examples Note: The following application examples are drawn from a selection of publications citing this product. For additional applications, please refer to the full list of references in the "Citations" section. [ERROR] Response ended prematurely |
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