Recombinant Human Basigin (BSG), partial (Active) CSB-MP002831HU1 CUSABIO
$9.90
In stock
SKU
CSB-MP002831HU1
Product Details
| Uniprot No. | P35613 |
| Alternative Names | (CD147)(TCSF)(EMMPRIN) |
| Species | Homo sapiens (Human) |
| Source | Mammalian cell |
| Expression Region | 22-205aa |
| Purity | Greater than 95% as determined by SDS-PAGE. |
| Endotoxin | Less than 1.0 EU/ug as determined by LAL method. |
| Activity | Measured by its binding ability in a functional ELISA. Immobilized CD147 at 2 μg/ml can bind Anti-CD147 recombinant Antibody, the EC 50 is 21.95-33.12 ng/ml. |
| Tag Info | C-terminal hFc1-tagged |
| Form | Lyophilized powder Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand. |
| Buffer | Lyophilized from a 0.2 μm filtered PBS, 6% Trehalose, pH 7.4 |
| Reconstitution | We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference. |
| Troubleshooting and FAQs | Protein FAQs |
| Notes | Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week. |
| Lead Time | 3-7 business days |
| Target Names | BSG |
| Target Protein Sequence | AAGTVFTTVEDLGSKILLTCSLNDSATEVTGHRWLKGGVVLKEDALPGQKTEFKVDSDDQWGEYSCVFLPEPMGTANIQLHGPPRVKAVKSSEHINEGETAMLVCKSESVPPVTDWAWYKITDSEDKALMNGSESRFFVSSSQGRSELHIENLNMEADPGQYRCNGTSSKGSDQAIITLRVRSH |
| Mol. Weight | 49.0 kDa |
| Protein Length | Partial of Isoform 2 |
| Storage Condition | Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles. |
| Shelf Life | The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C. |
| Datasheet & COA | Please contact us to get it. |
| Description | Basigin (CD147) drives tumor invasion and metastasis through its roles in matrix metalloproteinase induction and integrin-mediated adhesion, making it a critical target in cancer biology. This recombinant human BSG construct spans residues 22–205 of Isoform 2, covering the key extracellular immunoglobulin-like domains, and carries a C-terminal hFc1 tag that facilitates use in integrin-ligand binding studies, cell migration and invasion assays, and antibody development workflows. Functional ELISA confirms robust binding activity, with immobilized CD147 at 2 μg/ml engaging an anti-CD147 recombinant antibody at an EC50 of 21.95–33.12 ng/ml, providing a reliable basis for cell adhesion and spreading assays as well as tumor metastasis research. Mammalian cell expression preserves native glycosylation and folding essential for physiologically relevant protein–protein interactions, while purity exceeding 95% by SDS-PAGE and endotoxin levels below 1.0 EU/μg satisfy the criteria typically required for sensitive cell-based functional assays. |
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