Human Arachidonate 12-lipoxygenase, 12S-type (ALOX12/LOG12) ELISA kit CSB-E16996h CUSABIO
$50.00
In stock
SKU
CSB-E16996h
Product Details
| Target Name | arachidonate 12-lipoxygenase |
| Uniprot No. | P18054 |
| Species | Homo sapiens (Human) |
| Storage | Store at 2-8°C. Please refer to protocol. |
| Sensitivity | 0.31 ng/mL |
| Troubleshooting and FAQs | ELISA kit FAQs |
| Lead Time | 3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx. |
| Associated names | 12 LOX ELISA Kit; 12(S) lipoxygenase ELISA Kit; 12-lipoxygenase ELISA Kit; 12LO ELISA Kit; 12S LOX ELISA Kit; 12S-lipoxygenase ELISA Kit; 12S-LOX ELISA Kit; 12S-type ELISA Kit; Alox12 ELISA Kit; Arachidonate 12 lipoxygenase ELISA Kit; Arachidonate 12 lipoxygenase, 12S type ELISA Kit; Arachidonate 12-lipoxygenase ELISA Kit; Arachidonate 12-oxidoreductase ELISA Kit; Lipoxin synthase 12-LO ELISA Kit; LOG12 ELISA Kit; LOX12_HUMAN ELISA Kit; P-12LO ELISA Kit; Platelet 12-LOX ELISA Kit; Platelet type lipoxygenase 12 ELISA Kit; platelet-type 12-lipoxygenase ELISA Kit; Platelet-type lipoxygenase 12 ELISA Kit |
| Abbreviation | ALOX12 |
| Sample Types | serum, plasma, cell culture supernates, tissue homogenates |
| Detection Range | 1.25 ng/mL-80 ng/mL |
| Assay Time | 1-5h |
| Sample Loading Volume | 50-100ul |
| Detection Wavelength | 450 nm |
| Research Area | Immunology |
| Assay Principle | quantitative |
| Measurement | Sandwich |
| Precision | headers: Intra-assay Precision (Precision within an assay): CV%<8%; rows: Intra-assay Precision (Precision within an assay): CV%<8%: Intra-assay Precision (Precision within an assay): CV%<8%; 1: Three samples of known concentration were tested twenty times on one plate to assess.; Intra-assay Precision (Precision within an assay): CV%<8%: Inter-assay Precision (Precision between assays): CV%<10%; 1: Three samples of known concentration were tested in twenty assays to assess.; |
| Linearity | headers: To assess the linearity of the assay, samples were spiked with high concentrations of human ALOX12 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.; rows: To assess the linearity of the assay, samples were spiked with high concentrations of human ALOX12 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.: To assess the linearity of the assay, samples were spiked with high concentrations of human ALOX12 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.; 2: Sample; 3: Serum(n=4); 1: 1:1; 2: Average %; 3: 92; 1: Range %; 2: 88-99; 1: 1:2; 2: Average %; 3: 86; 1: Range %; 2: 80-94; 1: 1:4; 2: Average %; 3: 87; 1: Range %; 2: 82-93; 1: 1:8; 2: Average %; 3: 92; 1: Range %; 2: 88-96 |
| Recovery | headers: The recovery of human ALOX12 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.; rows: The recovery of human ALOX12 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.: The recovery of human ALOX12 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.; 1: Sample Type; 2: Average % Recovery; 3: Range; 1: Serum (n=5); 2: 93; 3: 89-99; 1: EDTA plasma (n=4); 2: 98; 3: 95-100; ; |
| Typical Data | headers: These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.; rows: These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.: These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.; 1: ng/ml OD1 OD2 Average Corrected 80 2.402 2.455 2.429 2.322 40 1.599 1.684 1.642 1.535 20 0.814 0.902 0.858 0.751 10 0.505 0.546 0.526 0.419 5 0.352 0.367 0.360 0.253 2.5 0.246 0.259 0.253 0.146 1.25 0.174 0.177 0.176 0.069 0 0.103 0.110 0.107; 2: ng/ml; 3: OD1; 4: OD2; 5: Average; 6: Corrected; 8: 80; 9: 2.402; 10: 2.455; 11: 2.429; 12: 2.322; 14: 40; 15: 1.599; 16: 1.684; 17: 1.642; 18: 1.535; 20: 20; 21: 0.814; 22: 0.902; 23: 0.858; 24: 0.751; 26: 10; 27: 0.505; 28: 0.546; 29: 0.526; 30: 0.419; 32: 5; 33: 0.352; 34: 0.367; 35: 0.360; 36: 0.253; 38: 2.5; 39: 0.246; 40: 0.259; 41: 0.253; 42: 0.146; 44: 1.25; 45: 0.174; 46: 0.177; 47: 0.176; 48: 0.069; 50: 0; 51: 0.103; 52: 0.110; 53: 0.107; 1: ng/ml; 2: OD1; 3: OD2; 4: Average; 5: Corrected; 1: 80; 2: 2.402; 3: 2.455; 4: 2.429; 5: 2.322; 1: 40; 2: 1.599; 3: 1.684; 4: 1.642; 5: 1.535; 1: 20; 2: 0.814; 3: 0.902; 4: 0.858; 5: 0.751; 1: 10; 2: 0.505; 3: 0.546; 4: 0.526; 5: 0.419; 1: 5; 2: 0.352; 3: 0.367; 4: 0.360; 5: 0.253; 1: 2.5; 2: 0.246; 3: 0.259; 4: 0.253; 5: 0.146; 1: 1.25; 2: 0.174; 3: 0.177; 4: 0.176; 5: 0.069; 1: 0; 2: 0.103; 3: 0.110; 4: 0.107 |
| ELISA Data Analysis | ELISA Standard Curve & Curve Expert software |
| Shelf Life | 6 months |
| Description | This Human ALOX12 ELISA Kit was designed for the quantitative measurement of Human ALOX12 protein in serum, plasma, cell culture supernates, tissue homogenates . It is a Sandwich ELISA kit, its detection range is 1.25 ng/mL-80 ng/mL and the sensitivity is 0.31 ng/mL. |
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