Human CD177 antigen(CD177) ELISA kit CSB-EL004886HU CUSABIO
$50.00
In stock
SKU
CSB-EL004886HU
Product Details
| Target Name | CD177 molecule |
| Uniprot No. | Q8N6Q3 |
| Species | Homo sapiens (Human) |
| Storage | Store at 2-8°C. Please refer to protocol. |
| Sensitivity | 0.225 ng/mL |
| Troubleshooting and FAQs | ELISA kit FAQs |
| Lead Time | 3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx. |
| Associated names | CD177 ELISA Kit; CD177 antigen ELISA Kit; CD177 molecule ELISA Kit; CD177_HUMAN ELISA Kit; HNA-2a ELISA Kit; HNA2A ELISA Kit; Human neutrophil alloantigen 2a ELISA Kit; NB1 ELISA Kit; NB1 glycoprotein ELISA Kit; NB1 GP ELISA Kit; Polycythemia rubra vera 1 ELISA Kit; Polycythemia rubra vera protein 1 ELISA Kit; PRV-1 ELISA Kit; PRV1 ELISA Kit |
| Abbreviation | CD177 |
| Sample Types | serum, plasma, tissue homogenates, cell culture supernates |
| Detection Range | 0.9 ng/mL-60 ng/mL |
| Assay Time | 1-5h |
| Sample Loading Volume | 50-100ul |
| Detection Wavelength | 450 nm |
| Research Area | Immunology |
| Assay Principle | quantitative |
| Measurement | Sandwich |
| Precision | headers: Intra-assay Precision (Precision within an assay): CV%<8%; rows: Intra-assay Precision (Precision within an assay): CV%<8%: Intra-assay Precision (Precision within an assay): CV%<8%; 1: Three samples of known concentration were tested twenty times on one plate to assess.; Intra-assay Precision (Precision within an assay): CV%<8%: Inter-assay Precision (Precision between assays): CV%<10%; 1: Three samples of known concentration were tested in twenty assays to assess.; |
| Linearity | headers: To assess the linearity of the assay, samples were spiked with high concentrations of human CD177 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.; rows: To assess the linearity of the assay, samples were spiked with high concentrations of human CD177 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.: To assess the linearity of the assay, samples were spiked with high concentrations of human CD177 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.; 2: Sample; 3: Serum(n=4); 1: 1:1; 2: Average %; 3: 103; 1: Range %; 2: 95-108; 1: 1:2; 2: Average %; 3: 86; 1: Range %; 2: 80-92; 1: 1:4; 2: Average %; 3: 92; 1: Range %; 2: 87-96; 1: 1:8; 2: Average %; 3: 96; 1: Range %; 2: 90-102 |
| Recovery | headers: The recovery of human CD177 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.; rows: The recovery of human CD177 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.: The recovery of human CD177 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.; 1: Sample Type; 2: Average % Recovery; 3: Range; 1: Serum (n=5); 2: 98; 3: 94-100; 1: EDTA plasma (n=4); 2: 94; 3: 89-99; ; |
| Typical Data | headers: These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.; rows: These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.: These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.; 1: ng/ml OD1 OD2 Average Corrected 60 2.635 2.863 2.749 2.611 30 1.835 1.995 1.915 1.777 15 1.158 1.256 1.207 1.069 7.5 0.550 0.533 0.542 0.404 3.75 0.321 0.341 0.331 0.193 1.8 0.251 0.249 0.250 0.112 0.9 0.200 0.206 0.203 0.065 0 0.139 0.137 0.138; 2: ng/ml; 3: OD1; 4: OD2; 5: Average; 6: Corrected; 8: 60; 9: 2.635; 10: 2.863; 11: 2.749; 12: 2.611; 14: 30; 15: 1.835; 16: 1.995; 17: 1.915; 18: 1.777; 20: 15; 21: 1.158; 22: 1.256; 23: 1.207; 24: 1.069; 26: 7.5; 27: 0.550; 28: 0.533; 29: 0.542; 30: 0.404; 32: 3.75; 33: 0.321; 34: 0.341; 35: 0.331; 36: 0.193; 38: 1.8; 39: 0.251; 40: 0.249; 41: 0.250; 42: 0.112; 44: 0.9; 45: 0.200; 46: 0.206; 47: 0.203; 48: 0.065; 50: 0; 51: 0.139; 52: 0.137; 53: 0.138; 1: ng/ml; 2: OD1; 3: OD2; 4: Average; 5: Corrected; 1: 60; 2: 2.635; 3: 2.863; 4: 2.749; 5: 2.611; 1: 30; 2: 1.835; 3: 1.995; 4: 1.915; 5: 1.777; 1: 15; 2: 1.158; 3: 1.256; 4: 1.207; 5: 1.069; 1: 7.5; 2: 0.550; 3: 0.533; 4: 0.542; 5: 0.404; 1: 3.75; 2: 0.321; 3: 0.341; 4: 0.331; 5: 0.193; 1: 1.8; 2: 0.251; 3: 0.249; 4: 0.250; 5: 0.112; 1: 0.9; 2: 0.200; 3: 0.206; 4: 0.203; 5: 0.065; 1: 0; 2: 0.139; 3: 0.137; 4: 0.138 |
| ELISA Data Analysis | ELISA Standard Curve & Curve Expert software |
| Shelf Life | 6 months |
| Description | This Human CD177 ELISA Kit was designed for the quantitative measurement of Human CD177 protein in serum, plasma, tissue homogenates, cell culture supernates. It is a Sandwich ELISA kit, its detection range is 0.9 ng/mL-60 ng/mL and the sensitivity is 0.225 ng/mL . |
MDSD