Human CD177 antigen(CD177) ELISA kit CSB-EL004886HU CUSABIO

$50.00
In stock
SKU
CSB-EL004886HU

Product Details

Target NameCD177 molecule
Uniprot No.Q8N6Q3
SpeciesHomo sapiens (Human)
StorageStore at 2-8°C. Please refer to protocol.
Sensitivity0.225 ng/mL
Troubleshooting and FAQsELISA kit FAQs
Lead Time3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx.
Associated namesCD177 ELISA Kit; CD177 antigen ELISA Kit; CD177 molecule ELISA Kit; CD177_HUMAN ELISA Kit; HNA-2a ELISA Kit; HNA2A ELISA Kit; Human neutrophil alloantigen 2a ELISA Kit; NB1 ELISA Kit; NB1 glycoprotein ELISA Kit; NB1 GP ELISA Kit; Polycythemia rubra vera 1 ELISA Kit; Polycythemia rubra vera protein 1 ELISA Kit; PRV-1 ELISA Kit; PRV1 ELISA Kit
AbbreviationCD177
Sample Typesserum, plasma, tissue homogenates, cell culture supernates
Detection Range0.9 ng/mL-60 ng/mL
Assay Time1-5h
Sample Loading Volume50-100ul
Detection Wavelength450 nm
Research AreaImmunology
Assay Principlequantitative
MeasurementSandwich
Precisionheaders: Intra-assay Precision (Precision within an assay): CV%<8%; rows: Intra-assay Precision (Precision within an assay): CV%<8%: Intra-assay Precision (Precision within an assay): CV%<8%; 1: Three samples of known concentration were tested twenty times on one plate to assess.; Intra-assay Precision (Precision within an assay): CV%<8%: Inter-assay Precision (Precision between assays): CV%<10%; 1: Three samples of known concentration were tested in twenty assays to assess.;
Linearityheaders: To assess the linearity of the assay, samples were spiked with high concentrations of human CD177 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.; rows: To assess the linearity of the assay, samples were spiked with high concentrations of human CD177 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.: To assess the linearity of the assay, samples were spiked with high concentrations of human CD177 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.; 2: Sample; 3: Serum(n=4); 1: 1:1; 2: Average %; 3: 103; 1: Range %; 2: 95-108; 1: 1:2; 2: Average %; 3: 86; 1: Range %; 2: 80-92; 1: 1:4; 2: Average %; 3: 92; 1: Range %; 2: 87-96; 1: 1:8; 2: Average %; 3: 96; 1: Range %; 2: 90-102
Recoveryheaders: The recovery of human CD177 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.; rows: The recovery of human CD177 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.: The recovery of human CD177 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.; 1: Sample Type; 2: Average % Recovery; 3: Range; 1: Serum (n=5); 2: 98; 3: 94-100; 1: EDTA plasma (n=4); 2: 94; 3: 89-99; ;
Typical Dataheaders: These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.; rows: These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.: These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.; 1: ng/ml OD1 OD2 Average Corrected 60 2.635 2.863 2.749 2.611 30 1.835 1.995 1.915 1.777 15 1.158 1.256 1.207 1.069 7.5 0.550 0.533 0.542 0.404 3.75 0.321 0.341 0.331 0.193 1.8 0.251 0.249 0.250 0.112 0.9 0.200 0.206 0.203 0.065 0 0.139 0.137 0.138; 2: ng/ml; 3: OD1; 4: OD2; 5: Average; 6: Corrected; 8: 60; 9: 2.635; 10: 2.863; 11: 2.749; 12: 2.611; 14: 30; 15: 1.835; 16: 1.995; 17: 1.915; 18: 1.777; 20: 15; 21: 1.158; 22: 1.256; 23: 1.207; 24: 1.069; 26: 7.5; 27: 0.550; 28: 0.533; 29: 0.542; 30: 0.404; 32: 3.75; 33: 0.321; 34: 0.341; 35: 0.331; 36: 0.193; 38: 1.8; 39: 0.251; 40: 0.249; 41: 0.250; 42: 0.112; 44: 0.9; 45: 0.200; 46: 0.206; 47: 0.203; 48: 0.065; 50: 0; 51: 0.139; 52: 0.137; 53: 0.138; 1: ng/ml; 2: OD1; 3: OD2; 4: Average; 5: Corrected; 1: 60; 2: 2.635; 3: 2.863; 4: 2.749; 5: 2.611; 1: 30; 2: 1.835; 3: 1.995; 4: 1.915; 5: 1.777; 1: 15; 2: 1.158; 3: 1.256; 4: 1.207; 5: 1.069; 1: 7.5; 2: 0.550; 3: 0.533; 4: 0.542; 5: 0.404; 1: 3.75; 2: 0.321; 3: 0.341; 4: 0.331; 5: 0.193; 1: 1.8; 2: 0.251; 3: 0.249; 4: 0.250; 5: 0.112; 1: 0.9; 2: 0.200; 3: 0.206; 4: 0.203; 5: 0.065; 1: 0; 2: 0.139; 3: 0.137; 4: 0.138
ELISA Data AnalysisELISA Standard Curve & Curve Expert software
Shelf Life6 months
DescriptionThis Human CD177 ELISA Kit was designed for the quantitative measurement of Human CD177 protein in serum, plasma, tissue homogenates, cell culture supernates. It is a Sandwich ELISA kit, its detection range is 0.9 ng/mL-60 ng/mL and the sensitivity is 0.225 ng/mL .

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