CDC37 Recombinant Monoclonal Antibody CSB-RA964136A0HU CUSABIO
$210.00
In stock
SKU
CSB-RA964136A0HU
Product Details
| Uniprot No. | Q16543 |
| Alternative Names | Hsp90 co-chaperone Cdc37 (Hsp90 chaperone protein kinase-targeting subunit) (p50Cdc37) [Cleaved into: Hsp90 co-chaperone Cdc37, N-terminally processed], CDC37, CDC37A |
| Species Reactivity | Human |
| Immunogen Species | Homo sapiens (Human) |
| Immunogen | A synthesized peptide derived from human Cdc37 |
| Clonality | Monoclonal |
| Isotype | Rabbit IgG |
| Purification Method | Affinity-chromatography |
| Concentration | It differs from different batches. Please contact us to confirm it. |
| Form | Liquid |
| Buffer | Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol. |
| Storage | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. |
| Tested Applications | ELISA, WB, FC |
| Recommended Dilution | headers: Application; Recommended Dilution; rows: Application: WB; Recommended Dilution: 1:500-1:5000; Application: FC; Recommended Dilution: 1:20-1:200 |
| Troubleshooting and FAQs | Antibody FAQs |
| Lead Time | Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time. |
| Usage | For Research Use Only. Not for use in diagnostic or therapeutic procedures. |
| Target Names | CDC37 |
| Conjugate | Non-conjugated |
| Clone No. | 10C3 |
| Protocols | ELISA Protocol Western Blotting(WB) Protocol Flow Cytometry (FC) Protocol |
| Description | CDC37 functions as an essential co-chaperone that partners with Hsp90 to facilitate the maturation and stabilization of protein kinases, making it a critical node in cellular signaling networks. By recruiting client kinases to the Hsp90 chaperone machinery, CDC37 influences pathways governing cell proliferation, survival, and stress responses. This regulatory role has positioned CDC37 as a protein of significant interest in studies exploring kinase-dependent signaling and chaperone biology. This recombinant monoclonal antibody, clone 10C3, offers researchers the reproducibility and consistency that comes with sequence-defined production. Unlike traditional hybridoma-derived antibodies, recombinant technology ensures that each lot performs identically to the last, eliminating the variability that can complicate long-term studies or multi-site collaborations. The antibody is raised in rabbit against a synthetic peptide derived from human CDC37 and has been affinity-purified for optimal specificity. Validation studies demonstrate robust performance across multiple experimental platforms. In western blot applications, the antibody reliably detects CDC37 in a diverse panel of human cell lines including HeLa, Jurkat, MCF-7, HEK293, K562, and PC-3, with recommended dilutions ranging from 1:500 to 1:5000. The observed band size of approximately 50 kDa runs slightly higher than the predicted 45 kDa molecular weight, a shift commonly attributed to post-translational modifications such as phosphorylation. Flow cytometry validation in HeLa cells confirms the antibody's utility for intracellular detection, with dilutions of 1:20 to 1:200 providing clear signal separation from isotype controls. This antibody supports investigations in epigenetics, nuclear signaling, and broader cell biology research where understanding chaperone-kinase interactions is essential. |
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