Human Cartilage intermediate layer protein 1(CILP) ELISA kit CSB-EL005437HU CUSABIO
$50.00
In stock
SKU
CSB-EL005437HU
Product Details
| Target Name | cartilage intermediate layer protein, nucleotide pyrophosphohydrolase |
| Uniprot No. | O75339 |
| Species | Homo sapiens (Human) |
| Storage | Store at 2-8°C. Please refer to protocol. |
| Sensitivity | 23.4 pg/mL |
| Troubleshooting and FAQs | ELISA kit FAQs |
| Lead Time | 3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx. |
| Associated names | Cartilage intermediate layer protein 1 ELISA Kit; Cartilage intermediate layer protein 1 C1 ELISA Kit; Cartilage intermediate layer protein 1 C2 ELISA Kit; cartilage intermediate layer protein ELISA Kit; cartilage intermediate layer protein; nucleotide pyrophosphohydrolase ELISA Kit; Cartilage intermediate-layer protein ELISA Kit; CILP 1 ELISA Kit; CILP ELISA Kit; CILP-1 ELISA Kit; CILP1_HUMAN ELISA Kit; HGNC:1980 ELISA Kit; HsT18872 ELISA Kit |
| Abbreviation | CILP |
| Sample Types | serum, plasma, tissue homogenates |
| Detection Range | 93.75 pg/mL-6000 pg/mL |
| Assay Time | 1-5h |
| Sample Loading Volume | 50-100ul |
| Detection Wavelength | 450 nm |
| Research Area | Signal Transduction |
| Assay Principle | quantitative |
| Measurement | Sandwich |
| Precision | headers: Intra-assay Precision (Precision within an assay): CV%<8%; rows: Intra-assay Precision (Precision within an assay): CV%<8%: Intra-assay Precision (Precision within an assay): CV%<8%; 1: Three samples of known concentration were tested twenty times on one plate to assess.; Intra-assay Precision (Precision within an assay): CV%<8%: Inter-assay Precision (Precision between assays): CV%<10%; 1: Three samples of known concentration were tested in twenty assays to assess.; |
| Linearity | headers: To assess the linearity of the assay, samples were spiked with high concentrations of human CILP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.; rows: To assess the linearity of the assay, samples were spiked with high concentrations of human CILP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.: To assess the linearity of the assay, samples were spiked with high concentrations of human CILP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.; 2: Sample; 3: Serum(n=4); 1: 1:5; 2: Average %; 3: 94; 1: Range %; 2: 90-98; 1: 1:10; 2: Average %; 3: 105; 1: Range %; 2: 100-112; 1: 1:20; 2: Average %; 3: 84; 1: Range %; 2: 80-88; 1: 1:40; 2: Average %; 3: 95; 1: Range %; 2: 90-100 |
| Recovery | headers: The recovery of human CILP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.; rows: The recovery of human CILP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.: The recovery of human CILP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.; 1: Sample Type; 2: Average % Recovery; 3: Range; 1: Serum (n=5); 2: 93; 3: 89-98; 1: EDTA plasma (n=4); 2: 98; 3: 94-106; ; |
| Typical Data | headers: These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.; rows: These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.: These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.; 1: pg/ml OD1 OD2 Average Corrected 6000 2.098 2.201 2.150 2.027 3000 1.512 1.454 1.483 1.360 1500 0.983 0.934 0.959 0.836 750 0.611 0.643 0.627 0.504 375 0.330 0.338 0.334 0.211 187.5 0.282 0.302 0.292 0.169 93.75 0.192 0.207 0.200 0.077 0 0.125 0.120 0.123; 2: pg/ml; 3: OD1; 4: OD2; 5: Average; 6: Corrected; 8: 6000; 9: 2.098; 10: 2.201; 11: 2.150; 12: 2.027; 14: 3000; 15: 1.512; 16: 1.454; 17: 1.483; 18: 1.360; 20: 1500; 21: 0.983; 22: 0.934; 23: 0.959; 24: 0.836; 26: 750; 27: 0.611; 28: 0.643; 29: 0.627; 30: 0.504; 32: 375; 33: 0.330; 34: 0.338; 35: 0.334; 36: 0.211; 38: 187.5; 39: 0.282; 40: 0.302; 41: 0.292; 42: 0.169; 44: 93.75; 45: 0.192; 46: 0.207; 47: 0.200; 48: 0.077; 50: 0; 51: 0.125; 52: 0.120; 53: 0.123; 1: pg/ml; 2: OD1; 3: OD2; 4: Average; 5: Corrected; 1: 6000; 2: 2.098; 3: 2.201; 4: 2.150; 5: 2.027; 1: 3000; 2: 1.512; 3: 1.454; 4: 1.483; 5: 1.360; 1: 1500; 2: 0.983; 3: 0.934; 4: 0.959; 5: 0.836; 1: 750; 2: 0.611; 3: 0.643; 4: 0.627; 5: 0.504; 1: 375; 2: 0.330; 3: 0.338; 4: 0.334; 5: 0.211; 1: 187.5; 2: 0.282; 3: 0.302; 4: 0.292; 5: 0.169; 1: 93.75; 2: 0.192; 3: 0.207; 4: 0.200; 5: 0.077; 1: 0; 2: 0.125; 3: 0.120; 4: 0.123 |
| ELISA Data Analysis | ELISA Standard Curve & Curve Expert software |
| Shelf Life | 6 months |
| Description | Cartilage intermediate layer protein 1 (CILP) is a secreted glycoprotein found primarily in the intermediate layer of articular cartilage. This protein functions as a nucleotide pyrophosphohydrolase and plays an important role in cartilage matrix organization and maintenance. CILP has been linked to various cartilage-related disorders and osteoarthritis pathogenesis, making it a significant biomarker for joint health research. Its expression levels can offer insights into cartilage metabolism and degenerative processes affecting joint function. The Human Cartilage intermediate layer protein 1 ELISA kit (CSB-EL005437HU) uses a quantitative sandwich measurement principle for detecting CILP in human samples. This assay works with serum, plasma, and tissue homogenates with a detection range of 93.75 pg/mL to 6000 pg/mL and shows a sensitivity of 23.4 pg/mL. The protocol requires 50-100 μL sample volume and can be completed within 1-5 hours, with detection performed at 450 nm wavelength. Application Examples Note: The following application examples are drawn from a selection of publications citing this product. For additional applications, please refer to the full list of references in the "Citations" section. This ELISA kit has been used in cardiovascular disease research to measure circulating cartilage intermediate layer protein levels in patient populations. Studies have applied the kit to examine biomarker associations in heart failure patients and to compare protein concentrations between different clinical groups. The kit has also been used in dermatological research examining hidradenitis suppurativa patients. • Cardiovascular research: Measuring circulating biomarker levels in heart failure patients with reduced ejection fraction and comparison with control populations • Clinical biomarker studies: Examining protein concentration differences between patient groups and healthy individuals in cardiovascular disease contexts • Dermatological research: Measuring serum protein levels in hidradenitis suppurativa patients to explore disease-associated biomarkers • Comparative assay validation: Testing kit performance alongside other commercial immunoassays to establish measurement correlations and analytical reliability |
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