Human Complement 4,C4 ELISA Kit CSB-E08705h CUSABIO
$50.00
In stock
SKU
CSB-E08705h
Product Details
| Target Name | Complement 4,C4 |
| Uniprot No. | P0C0L4 |
| Species | Homo sapiens (Human) |
| Storage | Store at 2-8°C. Please refer to protocol. |
| Sensitivity | 0.41 ng/mL |
| Troubleshooting and FAQs | ELISA kit FAQs |
| Lead Time | 3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx. |
| Associated names | Acidic C4 ELISA Kit; Acidic complement C4 ELISA Kit; Basic C4 ELISA Kit; Basic complement C4 ELISA Kit; C3 and PZP-like alpha-2-macroglobulin domain-containing protein 2 ELISA Kit; C3 and PZP-like alpha-2-macroglobulin domain-containing protein 3 ELISA Kit; C4; Chido form ELISA Kit; C4; Rodgers from ELISA Kit; C4A anaphylatoxin ELISA Kit; C4A ELISA Kit; C4A2 ELISA Kit; C4A3 ELISA Kit; C4A4 ELISA Kit; C4A6 ELISA Kit; C4AD ELISA Kit; C4B ELISA Kit; C4B_2 ELISA Kit; C4B1 ELISA Kit; C4B12 ELISA Kit; C4B2 ELISA Kit; C4B3 ELISA Kit; C4BD ELISA Kit; C4F ELISA Kit; C4S ELISA Kit; CH ELISA Kit; Chido form of C4 ELISA Kit; CO4 ELISA Kit; CO4A_HUMAN ELISA Kit; Complement C4 A ELISA Kit; Complement C4 B ELISA Kit; Complement C4 gamma chain ELISA Kit; complement C4-A ELISA Kit; complement C4-B ELISA Kit; complement C4-B-like ELISA Kit; complement C4B1a ELISA Kit; Complement component 4A (Rodgers blood group) ELISA Kit; Complement component 4A ELISA Kit; complement component 4B (Chido blood group) ELISA Kit; complement component 4B (Chido blood group); copy 2 ELISA Kit; Complement component 4B (Childo blood group) ELISA Kit; Complement component 4B ELISA Kit; Complement component 4F ELISA Kit; Complement component 4S ELISA Kit; CPAMD2 ELISA Kit; CPAMD3 ELISA Kit; RG ELISA Kit; Rodgers form of C4 ELISA Kit |
| Abbreviation | C4 |
| Sample Types | serum, plasma, tissue homogenates |
| Detection Range | 0.41 ng/mL-300 ng/mL |
| Assay Time | 1-5h |
| Sample Loading Volume | 50-100ul |
| Detection Wavelength | 450 nm |
| Research Area | Others |
| Assay Principle | quantitative |
| Measurement | Sandwich |
| Precision | headers: Intra-assay Precision (Precision within an assay): CV%<8%; rows: Intra-assay Precision (Precision within an assay): CV%<8%: Intra-assay Precision (Precision within an assay): CV%<8%; 1: Three samples of known concentration were tested twenty times on one plate to assess.; Intra-assay Precision (Precision within an assay): CV%<8%: Inter-assay Precision (Precision between assays): CV%<10%; 1: Three samples of known concentration were tested in twenty assays to assess.; |
| Linearity | headers: To assess the linearity of the assay, samples were spiked with high concentrations of human C4 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.; rows: To assess the linearity of the assay, samples were spiked with high concentrations of human C4 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.: To assess the linearity of the assay, samples were spiked with high concentrations of human C4 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.; 2: Sample; 3: Serum(n=4); 1: 1:1000; 2: Average %; 3: 96; 1: Range %; 2: 93-99; 1: 1:2000; 2: Average %; 3: 100; 1: Range %; 2: 97-103; 1: 1:4000; 2: Average %; 3: 89; 1: Range %; 2: 86-92; 1: 1:8000; 2: Average %; 3: 100; 1: Range %; 2: 97-104 |
| Recovery | headers: The recovery of human C4 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.; rows: The recovery of human C4 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.: The recovery of human C4 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.; 1: Sample Type; 2: Average % Recovery; 3: Range; 1: Serum (n=5); 2: 86; 3: 83-90; 1: EDTA plasma (n=4); 2: 95; 3: 91-99; ; |
| Typical Data | headers: These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.; rows: These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.: These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.; 1: ng/ml OD1 OD2 Average Corrected 300 2.873 2.897 2.885 2.786 100 1.916 1.968 1.942 1.843 33.3 1.281 1.299 1.290 1.191 11.1 0.878 0.898 0.888 0.789 3.7 0.495 0.472 0.484 0.385 1.23 0.195 0.212 0.204 0.105 0.41 0.187 0.192 0.190 0.091 0 0.101 0.097 0.099; 2: ng/ml; 3: OD1; 4: OD2; 5: Average; 6: Corrected; 8: 300; 9: 2.873; 10: 2.897; 11: 2.885; 12: 2.786; 14: 100; 15: 1.916; 16: 1.968; 17: 1.942; 18: 1.843; 20: 33.3; 21: 1.281; 22: 1.299; 23: 1.290; 24: 1.191; 26: 11.1; 27: 0.878; 28: 0.898; 29: 0.888; 30: 0.789; 32: 3.7; 33: 0.495; 34: 0.472; 35: 0.484; 36: 0.385; 38: 1.23; 39: 0.195; 40: 0.212; 41: 0.204; 42: 0.105; 44: 0.41; 45: 0.187; 46: 0.192; 47: 0.190; 48: 0.091; 50: 0; 51: 0.101; 52: 0.097; 53: 0.099; 1: ng/ml; 2: OD1; 3: OD2; 4: Average; 5: Corrected; 1: 300; 2: 2.873; 3: 2.897; 4: 2.885; 5: 2.786; 1: 100; 2: 1.916; 3: 1.968; 4: 1.942; 5: 1.843; 1: 33.3; 2: 1.281; 3: 1.299; 4: 1.290; 5: 1.191; 1: 11.1; 2: 0.878; 3: 0.898; 4: 0.888; 5: 0.789; 1: 3.7; 2: 0.495; 3: 0.472; 4: 0.484; 5: 0.385; 1: 1.23; 2: 0.195; 3: 0.212; 4: 0.204; 5: 0.105; 1: 0.41; 2: 0.187; 3: 0.192; 4: 0.190; 5: 0.091; 1: 0; 2: 0.101; 3: 0.097; 4: 0.099 |
| Materials provided | A micro ELISA plate ---The 96-well plate has been pre-coated with an anti-human C4 antibody. Two vials lyophilized standard ---Dilute a bottle of the standard at dilution series, read the OD values, and then draw a standard curve. One vial HRP-conjugated C4 antibody (100 x concentrate) (120 μl/bottle) ---Bind to the C4 in the samples or standards and react with the substrate to make the solution chromogenic. One HRP-conjugate Diluent(20 ml/bottle) ---Dilute the HRP-conjugated C4 antibody Two vials Sample Diluent (20 ml/bottle) ---Dilute the sample solution. One vial Wash Buffer (25x concentrate) (20ml/bottle) ---Wash away unbound or free substances. One vial TMB Substrate (10 ml/bottle) ---Act as the chromogenic agent. TMB interacts with HRP, eliciting the solution turns blue. One vial Stop Solution (7ml/bottle) ---Stop the color reaction. The solution color immediately turns from blue to yellow. Four Adhesive Strips (For 96 wells) ---Cover the microplate when incubation. An instruction manual |
| Materials not provided | A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 600 nm or 630 nm. An incubator can provide stable incubation conditions up to 37°C±5°C. Centrifuge Vortex Squirt bottle, manifold dispenser, or automated microplate washer Absorbent paper for blotting the microtiter plate 50-300ul multi-channel micropipette Pipette tips Single-channel micropipette with different ranges 100ml and 500ml graduated cylinders Deionized or distilled water Timer Test tubes for dilution |
| ELISA Data Analysis | ELISA Standard Curve & Curve Expert software |
| Shelf Life | 6 months |
| Description | This Human Complement 4 (C4) ELISA Kit was designed for the quantitative measurement of Human Complement 4 (C4) protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 0.41 ng/mL-300 ng/mL and the sensitivity is 0.41 ng/mL. |
MDSD
Reviews
Write Your Own Review
Related Products
Check items to add to the cart or
