NDUFA12 Polyclonal Antibody (E-AB-18397) elabscience
$399.00
In stock
SKU
E-AB-18397
| Size | Price (USD) |
| 200μL | $399.00 |
| 120μL | $240.00 |
| 60μL | $143.00 |
| 20μL | $73.00 |
Product Details
| Abbre | NDUFA12 |
| Synonyms | 13 kDa differentiation associated protein, 13 kDa differentiation-associated protein, 2410011G03Rik, AW112974, B17.2, CI-B17.2, CIB17.2, Complex I B17.2, Complex I B17.2 subunit, Complex I-B17.2, DAP13, MGC107642, MGC7999, NADH dehydrogenase (ubiquinone) 1 alpha subco |
| Swissprot | Q9UI09 |
| Reactivity | Human, Mouse |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | WB, IHC |
| Conjugation | Unconjugated |
| Concentration | 0.7 mg/mL |
| Buffer | Phosphate buffered solution, pH 7.4, containing 0.05% stabilizer and 50% glycerol. |
| Purification Method | Antigen affinity purification |
| Immunogen | Full length fusion protein |
| Calculated MW | 17 kDa |
| Observed MW | Refer to figures The actual band is not consistent with the expectation. Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include: 1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein. 2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes. 3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1. 4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids). 5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers. If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane. |
| Research Areas | Cancer, Metabolism, Signal Transduction |
| Cellular Localization | Mitochondrion inner membrane. |
Product Description
Background: This gene encodes a protein which is part of mitochondrial complex 1, part of the oxidative phosphorylation system in mitochondria. Complex 1 transfers electrons to ubiquinone from NADH which establishes a proton gradient for the generation of ATP. Mutations in this gene are associated with Leigh syndrome due to mitochondrial complex 1 deficiency. Pseudogenes of this gene are located on chromosomes 5 and 13. Alternative splicing results in multiple transcript variants.
Storage and Shipping
| Storage | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. |
| Shipping | The product is shipped with ice pack,upon receipt,store it immediately at the temperature recommended. |
Additional Information
| Verified Samples | Verified Samples in WB: Human fetal muscle, PC-3, Jurkat Verified Samples in IHC: Human brain, Human lung cancer |
| Dilution | WB 1:500-1:2000, IHC 1:25-1:100 |
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