Triglyceride (TG) Colorimetric Assay Kit (Single Reagent, GPO-PAP Method) (E-BC-K261-M) elabscience
$360.00
In stock
SKU
E-BC-K261-M
500Assays $ 360.00
Product Details
| Synonyms | TG |
| Research Area | Metabolic Diseases , Glycolysis And Lipid Metabolism , Energy Metabolism |
Product Description
Detection Principle: Triglycerides (TG) can be hydrolyzed by lipoprotein lipase into glycerol and free fatty acids. Glycerol produces glycerol-3-phosphate and ADP under the catalysis of glycerol kinase (GK). Glycerol-3-phosphate produces hydrogen peroxide under the action of glycerol phosphate oxidase (GPO). In the presence of trinder’s reagent, hydrogen peroxide is catalyzed by peroxidase to produce quinones which is proportional to the content of TG.
Assay / Application Information
| Detection Method | Colorimetric method |
| Detection Range | 0.14-10 mmol/L |
| Sensitivity | 0.14 mmol/L |
| Sample Type | Serum,Plasma,Tissue |
| Sample Volume | 2.5 μL |
| Precision | inter-assay CV: 9.2% | intra-assay CV: 4.1% |
| Assay Time | 30 min |
| Valid Period | 12 months |
| Detection Instrument | Microplate reader (510 nm) |
| Other Reagents Required | Normal saline (0.9% NaCl), PBS (0.01 mol/L, pH 7.4), Isopropanol (AR), Anhydrous ethanol |
Storage and Shipping
| Storage | This product can be stored at 2-8°C for 12 months with shading light. |
Sample Information
| Human serum | 1 |
| Mouse serum | 1 |
| Rat plasma | 1 |
| 10% Mouse liver tissue homogenate | 1 |
| 10% Mouse kidney tissue homogenate | 1 |
| 10% Mouse heart tissue homogenate | 1 |
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