Recombinant RNase Inhibitor (Murine)(Cat. No.:E125) novoprotein
$50.00
In stock
SKU
E125
3000U: Inquiry
15KU: Inquiry
Description
Recombinant RNase Inhibitor (Murine) is a 50 kDa recombinant protein of murine origin. The inhibitor specifically inhibits RNases A, B and C. It inhibits RNases by binding noncovalently in a 1:1 ratio with high affinity. It is not effective against RNase 1, RNase T1, S1 Nuclease, RNase H or RNase from Aspergillus. In addition, no inhibition of polymerase activity is observed when RNase Inhibitor is used with Taq DNA Polymerase, AMV or M-MuLV Reverse Transcriptases, or Phage RNA Polymerases (SP6, T7, or T3).
Recombinant RNase Inhibitor (Murine) does not contain the pair of cysteines identified in the human version that is very sensitive to oxidation, which causes inactivation of the inhibitor. As a result, Recombinant RNase Inhibitor (Murine) has significantly improved resistance to oxidation compared to the human/porcine RNase inhibitors, and is stable at low DTT concentrations (less than 1 mM). This makes it ideal for reactions where high concentration DTT is adverse to the reaction (eg. Real-time RT-PCR).
Quality Control
After multiple column purifications, only a clear single target band was visible in SDS-PAGE gel detection; qPCR method detection showed no residual E. coli DNA and no endonuclease or exonuclease contamination.
Product Details
| Item | Details |
| Storage | -20℃. |
Components
| Component | E125-01A （Size：3000U） | E125-01B （Size：15KU） |
| Recombinant RNase Inhibitor (Murine, 40U/μl) | 75μl | 375μl |
Notes
Recombinant RNase Inhibitor (Murine) is active over a wide pH range (pH5.0-9.0), maximum activity at pH7.0-8.0.
Inactivation: Temperature higher than 65℃ or intense denaturation conditions for inactivation.
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