C9orf61 Polyclonal antibody 25630-1-AP proteintech
$149.00
In stock
SKU
25630-1-AP
| Catalog Number | 25630-1-AP |
| Synonyms | Endosomal transmembrane binding with epsin, Endosomal transmembrane epsin interactor 1, ENTREP1, FAM189A2, Protein FAM189A2 |
| Host | Rabbit |
| Reactivity | human, mouse |
| Form | Liquid |
| Formulation | PBS, Azide, Glycerol |
| Applications | WB, IHC, IF, CoIP, ELISA |
| Applications | WB, IHC, ELISA |
| Host / Isotype | Rabbit / IgG |
| Tested Applications — Positive WB detected in | mouse skeletal muscle tissue |
| Tested Applications — Positive IHC detected in | human heart tissue, human skeletal muscle tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Recommended Dilutions — Western Blot (WB) | WB : 1:500-1:1000 |
| Recommended Dilutions — Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Positive WB detected in | mouse skeletal muscle tissue |
| Positive IHC detected in | human heart tissue, human skeletal muscle tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Tested Reactivity | human, mouse |
| Cited Reactivity | human |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | CatNo: Ag22242 Product name: Recombinant human C9orf61 protein Source: e coli. -derived, PET30a Tag: 6*His Domain: 288-450 aa of BC113685 Sequence: PVLSCEAATQTERRLDLAAVTLRRGLRSRASRCRPRSLIDYKSYMDTKLLVARFLEQSSCTMTPDIHELVENIKSVLKSDEEHMEEAITSASFLEQIMAPLQPSTSRAHKLPSRRQPGLLHLQSCGDLHTFTPAGRPRAERRPRRVEAERPHSLIGVIRETVL Predict reactive species |
| Full Name | chromosome 9 open reading frame 61 |
| Calculated Molecular Weight | 450 aa, 50 kDa |
| Observed Molecular Weight | 45-50 kDa |
| GenBank Accession Number | BC113685 |
| Gene Symbol | C9orf61 |
| Gene ID (NCBI) | 9413 |
| RRID | AB_2880170 |
| Conjugate | Unconjugated |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q15884 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA. |
| IHC protocol for C9orf61 antibody 25630-1-AP | Download protocol |
| WB protocol for C9orf61 antibody 25630-1-AP | Download protocol |
| human | WB,IHC,IF J Transl Med FAM189A2 activates Hippo signaling pathway by abrogating WWP2-mediated LATS1 ubiquitination, to inhibit the glycolysis and proliferation processes of lung adenocarcinoma. Authors - Bingyu Chen View Article |
| Chrisovalantou (Verified Customer) (10-31-2024) | Antibody unfortunately could not reliably and specifically detect the protein of interest. Many unspecific bands. Applications: Western Blot Primary Antibody Dilution: 1:1000 Cell Tissue Type: SH-SY5Y, HEK293T C9orf61 Antibody Western Blot validation (1:1000 dilution) in SH-SY5Y, HEK293T (Cat no:25630-1-AP) |
| Citations | 2 |
| Dilutions | WB : 1:500-1:1000 IHC : 1:20-1:200 |
| Isotype | IgG |
| Clonality | Polyclonal |
| Clone Number | - |
| Conjugation | Unconjugated |
C9orf61, also named as FAM189A2 and X123, is promoinently expressed in muscle. It has three isoforms with MW 45-50 kDa, 32-35 kDa and 27 kDa. Protocols Product Specific Protocols IHC protocol for C9orf61 antibody 25630-1-AP Download protocol WB protocol for C9orf61 antibody 25630-1-AP Download protocol Standard Protocols Click here to view our Standard Protocols
Publications
- FAM189A2 plays a tumour suppressor role in lung adenocarcinoma by influencing cell apoptosis, CXCR4 expression and tight junction proteins
Journal: Tissue Cell | Authors: Jiakun Liu | Species: human | Application: WB,IHC,CoIP - FAM189A2 activates Hippo signaling pathway by abrogating WWP2-mediated LATS1 ubiquitination, to inhibit the glycolysis and proliferation processes of lung adenocarcinoma.
Journal: J Transl Med | Authors: Bingyu Chen | Species: human | Application: WB,IHC,IF
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