CACYBP Monoclonal antibody 68161-1-Ig proteintech
$149.00
In stock
SKU
68161-1-Ig
| Catalog Number | 68161-1-Ig |
| Synonyms | Calcyclin, S100 alpha 6, 3C2G11, calcyclin binding protein, Calcyclin-binding protein |
| Host | Mouse |
| Reactivity | human, mouse, rat |
| Form | Liquid |
| Formulation | PBS, Azide, Glycerol |
| Applications | WB, IHC, IF/ICC, IP, ELISA |
| Host / Isotype | Mouse / IgG2a |
| Tested Applications — Positive WB detected in | HCT 116 cells, 4T1 cells, HEK-293 cells, LNCaP cells, HeLa cells, Jurkat cells, K-562 cells, HSC-T6 cells, NIH/3T3 cells |
| Tested Applications — Positive IP detected in | HeLa cells |
| Tested Applications — Positive IHC detected in | mouse brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Tested Applications — Positive IF/ICC detected in | HEK-293 cells |
| Recommended Dilutions — Western Blot (WB) | WB : 1:5000-1:50000 |
| Recommended Dilutions — Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Recommended Dilutions — Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| Recommended Dilutions — Immunofluorescence (IF)/ICC | IF/ICC : 1:400-1:1600 |
| Positive WB detected in | HCT 116 cells, 4T1 cells, HEK-293 cells, LNCaP cells, HeLa cells, Jurkat cells, K-562 cells, HSC-T6 cells, NIH/3T3 cells |
| Positive IP detected in | HeLa cells |
| Positive IHC detected in | mouse brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HEK-293 cells |
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:400-1:1600 |
| Tested Reactivity | human, mouse, rat |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | CatNo: Ag2340 Product name: Recombinant human CACYBP protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-228 aa of BC022352 Sequence: MASEELQKDLEEVKVLLEKATRKRVRDALTAEKSKIETEIKNKMQQKSQKKAELLDNEKPAAVVAPITTGYTVKISNYGWDQSDKFVKIYITLTGVHQVPTENVQVHFTERSFDLLVKNLNGKSYSMIVNNLLKPISVEGSSKKVKTDTVLILCRKKVENTRWDYLTQVEKECKEKEKPSYDTETDPSEGLMNVLKKIYEDGDDDMKRTINKAWVESREKQAKGDTEF Predict reactive species |
| Full Name | calcyclin binding protein |
| Calculated Molecular Weight | 228 aa, 26 kDa |
| Observed Molecular Weight | 26-30 kDa |
| GenBank Accession Number | BC022352 |
| Gene Symbol | CACYBP |
| Gene ID (NCBI) | 27101 |
| RRID | AB_2923680 |
| Conjugate | Unconjugated |
| Purification Method | Protein A purification |
| UNIPROT ID | Q9HB71 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA. |
| IF protocol for CACYBP antibody 68161-1-Ig | Download protocol |
| IHC protocol for CACYBP antibody 68161-1-Ig | Download protocol |
| IP protocol for CACYBP antibody 68161-1-Ig | Download protocol |
| WB protocol for CACYBP antibody 68161-1-Ig | Download protocol |
| Citations | 1 |
| Dilutions | WB : 1:5000-1:50000 IP : 0.5-4.0 ug for IP and 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate for WB IHC : 1:500-1:2000 IF/ICC : 1:400-1:1600 |
| Isotype | IgG2a |
| Clonality | Monoclonal |
| Clone Number | 3C2G11 |
| Conjugation | Unconjugated |
CACYBP(Calcyclin-binding protein) is also named as S100A6BP, SIP. It is a new target 30 kDa protein that can interact with S100 proteins, including S100A6, S100A1, S100B, and S100P, but not to S100A4, calbindin D, parvalbumin or calmodulin(PMID:22295074). It may be involved in calcium-dependent ubiquitination and subsequent proteasomal degradation of target proteins and probably serves as a molecular bridge in ubiquitin E3 complexe. It has 2 isoforms produced by alternative splicing and can exist as a dimer(PMID:17916393). Protocols Product Specific Protocols IF protocol for CACYBP antibody 68161-1-Ig Download protocol IHC protocol for CACYBP antibody 68161-1-Ig Download protocol IP protocol for CACYBP antibody 68161-1-Ig Download protocol WB protocol for CACYBP antibody 68161-1-Ig Download protocol Standard Protocols Click here to view our Standard Protocols
Publications
- LZTR1 is a melanoma oncogene that promotes invasion and suppresses apoptosis
Journal: Oncogene | Authors: Antonella Bacchiocchi
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