| Catalog Number | CL647-FcA65651 |
| Synonyms | 145-2C11, AI504783, CD3, CD3epsilon, CD3ε |
| Host | Rabbit |
| Reactivity | mouse |
| Form | Liquid |
| Formulation | PBS, Azide |
| Applications | FC |
| Host / Isotype | Rabbit / IgG |
| Tested Applications — Positive FC detected in | mouse splenocytes |
| Positive FC detected in | mouse splenocytes |
| Flow Cytometry (FC) | FC : 0.25 ug per 10^6 cells in a 100 µl suspension |
| Tested Reactivity | mouse |
| Class | Recombinant |
| Type | Antibody |
| Immunogen | N/A Predict reactive species |
| Full Name | CD3 antigen, epsilon polypeptide |
| GenBank Accession Number | BC098236 |
| Gene Symbol | CD3ε |
| Gene ID (NCBI) | 12501 |
| RRID | AB_3748898 |
| Conjugate | CoraLite® Plus 647 Fluorescent Dye |
| Excitation/Emission Maxima Wavelengths | 654 nm / 674 nm |
| Excitation Laser | Red Laser (633 nm) |
| Purification Method | Protein A purification |
| UNIPROT ID | P22646 |
| Storage Buffer | PBS with 0.09% sodium azide, pH 7.3. |
| Storage Conditions | Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment. |
| FC protocol for CL Plus 647 CD3e antibody CL647-FcA65651 | Download protocol |
| Citations | - |
| Isotype | IgG |
| Clonality | Recombinant |
| Clone Number | 145-2C11 |
| Conjugation | CoraLite® Plus 647 |
CD3 is a multimeric protein associated with the T-cell receptor (TCR) to form a complex involved in antigen recognition and signal transduction (PMID: 15885124). CD3 is composed of CD3γ, δ, ε, and ζ chains (PMID: 1826255). It is expressed by thymocytes in a developmentally regulated manner, T cells, and some NK cells (PMID: 3289580). The TCR recognizes antigens bound to major histocompatibility complex (MHC) molecules. TCR-mediated peptide-MHC recognition is transmitted to the CD3 complex, leading to the intracellular signal transduction (PMID: 11985657). Protocols Product Specific Protocols FC protocol for CL Plus 647 CD3e antibody CL647-FcA65651 Download protocol Standard Protocols Click here to view our Standard Protocols
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