| Catalog Number | 60232-1-Ig |
| Synonyms | CD9 antigen, BTCC 1, BA2, 5H9 antigen, 5H9 |
| Host | Mouse |
| Reactivity | human and More (1) |
| Reactivity | human, rabbit |
| Form | Liquid |
| Formulation | PBS, Azide, Glycerol |
| Applications | WB, IHC, IF/ICC, IF-P, ELISA, PLA |
| Applications | WB, IHC, IF/ICC, IF-P, ELISA |
| Host / Isotype | Mouse / IgG1 |
| Tested Applications — Positive WB detected in | A431 cells, HeLa cells |
| Tested Applications — Positive IHC detected in | human ovary tumor tissue, human breast cancer tissue, human colon cancer tissue, human tonsillitis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Tested Applications — Positive IF-P detected in | human breast cancer tissue, human ovary tumor tissue, human lung cancer tissue |
| Tested Applications — Positive IF/ICC detected in | MCF-7 cells |
| Recommended Dilutions — Western Blot (WB) | WB : 1:5000-1:50000 |
| Recommended Dilutions — Immunohistochemistry (IHC) | IHC : 1:1000-1:4000 |
| Recommended Dilutions — Immunofluorescence (IF)-P | IF-P : 1:200-1:800 |
| Recommended Dilutions — Immunofluorescence (IF)/ICC | IF/ICC : 1:400-1:1600 |
| Positive WB detected in | A431 cells, HeLa cells |
| Positive IHC detected in | human ovary tumor tissue, human breast cancer tissue, human colon cancer tissue, human tonsillitis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | human breast cancer tissue, human ovary tumor tissue, human lung cancer tissue |
| Positive IF/ICC detected in | MCF-7 cells |
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunohistochemistry (IHC) | IHC : 1:1000-1:4000 |
| Immunofluorescence (IF)-P | IF-P : 1:200-1:800 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:400-1:1600 |
| Tested Reactivity | human |
| Cited Reactivity | human, rabbit |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | CatNo: Ag14529 Product name: Recombinant human CD9 protein Source: e coli. -derived, PET28a Tag: 6*His Domain: 100-208 aa of BC011988 Sequence: FAIEIAAAIWGYSHKDEVIKEVQEFYKDTYNKLKTKDEPQRETLKAIHYALNCCGLAGGVEQFISDICPKKDVLETFTVKSCPDAIKEVFDNKFHIIGAVGIGIAVVMI Predict reactive species |
| Full Name | CD9 molecule |
| Calculated Molecular Weight | 228 aa, 25 kDa |
| Observed Molecular Weight | 23-27 kDa |
| GenBank Accession Number | BC011988 |
| Gene Symbol | CD9 |
| Gene ID (NCBI) | 928 |
| RRID | AB_11232215 |
| Conjugate | Unconjugated |
| Purification Method | Protein G purification |
| UNIPROT ID | P21926 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA. |
| IF protocol for CD9 antibody 60232-1-Ig | Download protocol |
| IHC protocol for CD9 antibody 60232-1-Ig | Download protocol |
| WB protocol for CD9 antibody 60232-1-Ig | Download protocol |
| human | WB Adv Sci (Weinh) Cancer Cell-Derived Large Extracellular Vesicles Promote Venous Thromboembolism by Activating NETosis Through Delivering CYBA Authors - Xiangji Li View Article |
| Guorong (Verified Customer) (03-22-2022) | Very weak bands with strong background were detected at 25 kDa Applications: Western Blot Primary Antibody Dilution: 1:5000 Cell Tissue Type: ARPE-19 CD9 Antibody Western Blot validation (1:5000 dilution) in ARPE-19 (Cat no:60232-1-Ig) |
| Citations | 175 |
| Dilutions | WB : 1:5000-1:50000 IHC : 1:1000-1:4000 IF-P : 1:200-1:800 IF/ICC : 1:400-1:1600 |
| Isotype | IgG1 |
| Clonality | Monoclonal |
| Clone Number | 4H7B9 |
| Conjugation | Unconjugated |
The cell-surface molecule CD9, a member of the transmembrane-4 superfamily, interacts with the integrin family and other membrane proteins, and is postulated to participate in cell migration and adhesion. Expression of CD9 enhances membrane fusion between muscle cells and promotes viral infection in some cells (PMID:10459022). It is often used as a mesenchymal stem cell marker (PMID:18005405). CD9 is also known as the p24 antigen besides MIC3, TSPAN29 because it is a protein of molecular weight 24 kD. The CD9 antigen appears to be a 227-amino acid molecule with 4 hydrophobic domains and 1 N-glycosylation site. Protocols Product Specific Protocols IF protocol for CD9 antibody 60232-1-Ig Download protocol IHC protocol for CD9 antibody 60232-1-Ig Download protocol WB protocol for CD9 antibody 60232-1-Ig Download protocol Standard Protocols Click here to view our Standard Protocols
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