DNAJC3 Polyclonal antibody 26721-1-AP proteintech
$149.00
In stock
SKU
26721-1-AP
| Catalog Number | 26721-1-AP |
| Synonyms | DNAJC3, HP58, P58, P58IPK, PRKRI, Protein kinase inhibitor p58 |
| Host | Rabbit |
| Reactivity | human, mouse |
| Form | Liquid |
| Formulation | PBS, Azide, Glycerol |
| Applications | WB, IHC, IF/ICC, ELISA |
| Host / Isotype | Rabbit / IgG |
| Tested Applications — Positive WB detected in | mouse testis tissue |
| Tested Applications — Positive IHC detected in | human colon cancer tissue, mouse testis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Tested Applications — Positive IF/ICC detected in | U2OS cells |
| Recommended Dilutions — Western Blot (WB) | WB : 1:500-1:1000 |
| Recommended Dilutions — Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Recommended Dilutions — Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| Positive WB detected in | mouse testis tissue |
| Positive IHC detected in | human colon cancer tissue, mouse testis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | U2OS cells |
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | CatNo: Ag24966 Product name: Recombinant human DNAJC3 protein Source: e coli. -derived, PET30a Tag: 6*His Domain: 32-380 aa of BC047936 Sequence: GVNADVEKHLELGKKLLAAGQLADALSQFHAAVDGDPDNYIAYYRRATVFLAMGKSKAALPDLTKVIQLKMDFTAARLQRGHLLLKQGKLDEAEDDFKKVLKSNPSENEEKEAQSQLIKSDEMQRLRSQALNAFGSGDYTAAIAFLDKILEVCVWDAELRELRAECFIKEGEPRKAISDLKAASKLKNDNTEAFYKISTLYYQLGDHELSLSEVRECLKLDQDHKRCFAHYKQVKKLNKLIESAEELIRDGRYTDATSKYESVMKTEPSIAEYTVRSKERICHCFSKDEKPVEAIRVCSEVLQMEPDNVNALKDRAEAYLIEEMYDEAIQDYETAQEHNENDQQIREGL Predict reactive species |
| Full Name | DnaJ (Hsp40) homolog, subfamily C, member 3 |
| Observed Molecular Weight | 58 kDa |
| GenBank Accession Number | BC047936 |
| Gene Symbol | DNAJC3 |
| Gene ID (NCBI) | 5611 |
| RRID | AB_2880613 |
| Conjugate | Unconjugated |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q13217 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA. |
| IF protocol for DNAJC3 antibody 26721-1-AP | Download protocol |
| IHC protocol for DNAJC3 antibody 26721-1-AP | Download protocol |
| WB protocol for DNAJC3 antibody 26721-1-AP | Download protocol |
| mouse | IHC Neural Regen Res Heat shock protein 40 enhances axon regeneration in a mouse model of traumatic optic neuropathy. Authors - Jiaxing Wang KD Validated View Article |
| human | WB FEBS Open Bio A systems biological analysis of the ATF4-GADD34-CHOP regulatory triangle upon endoplasmic reticulum stress Authors - Margita Márton View Article |
| Citations | 4 |
| Dilutions | WB : 1:500-1:1000 IHC : 1:50-1:500 IF/ICC : 1:50-1:500 |
| Isotype | IgG |
| Clonality | Polyclonal |
| Clone Number | - |
| Conjugation | Unconjugated |
DNAJC3, also known as P58,HP58 and P58IPK, belongs to heat shock protein family,which is a kind of conservative molecules that expresses extensively in cells of human and animals and protects cells against all kinds of damage. As a cochaperone of BiP that an HSP70 molecular chaperone located in the lumen of the endoplasmic reticulum(ER), DNAJC3 plays a crucial role in ER protein folding and the UPR(unfolded protein response) (PMID:10597629; PMID:25466870). Studies reported that DNAJC3 related several diseases such as osteosarcoma, widespread neurodegeneration, diabetes mellitus, sensorimotor neuropathy, ataxia, sensorineural hearing loss and short body stature(PMID:30652414; PMID:28940199). Protocols Product Specific Protocols IF protocol for DNAJC3 antibody 26721-1-AP Download protocol IHC protocol for DNAJC3 antibody 26721-1-AP Download protocol WB protocol for DNAJC3 antibody 26721-1-AP Download protocol Standard Protocols Click here to view our Standard Protocols
Publications
- UFMylation of HRD1 regulates endoplasmic reticulum homeostasis
Journal: FASEB J | Authors: Hui Luo | Species: mouse | Application: WB - Heat shock protein 40 enhances axon regeneration in a mouse model of traumatic optic neuropathy.
Journal: Neural Regen Res | Authors: Jiaxing Wang KD Validated | Species: mouse | Application: IHC - The role of DNAJC3 in enhancing glioma progression and regulating the tumor immune microenvironment.
Journal: J Biol Chem | Authors: Yinmin Shi KD Validated | Species: human | Application: WB - A systems biological analysis of the ATF4-GADD34-CHOP regulatory triangle upon endoplasmic reticulum stress
Journal: FEBS Open Bio | Authors: Margita Márton | Species: human | Application: WB
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