E2F7 Recombinant monoclonal antibody 82887-1-RR proteintech
$149.00
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SKU
82887-1-RR
| Catalog Number | 82887-1-RR |
| Synonyms | 230073C2, E2F-7, Transcription factor E2F7 |
| Host | Rabbit |
| Reactivity | human |
| Form | Liquid |
| Formulation | PBS, Azide, Glycerol |
| Applications | IF/ICC, FC (Intra), ELISA |
| Host / Isotype | Rabbit / IgG |
| Tested Applications — Positive IF/ICC detected in | HeLa cells |
| Tested Applications — Positive FC (Intra) detected in | HeLa cells |
| Recommended Dilutions — Immunofluorescence (IF)/ICC | IF/ICC : 1:500-1:2000 |
| Recommended Dilutions — Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension |
| Positive IF/ICC detected in | HeLa cells |
| Positive FC (Intra) detected in | HeLa cells |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:500-1:2000 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension |
| Tested Reactivity | human |
| Class | Recombinant |
| Type | Antibody |
| Immunogen | CatNo: Ag21599 Product name: Recombinant human E2F7 protein Source: e coli. -derived, PET30a Tag: 6*His Domain: 369-713 aa of BC136366 Sequence: VDFSSSDEELVDVSASVLPELKRETYGQIQVCAKQKLARHGSFNTVQASERIQRKVNSEPSSPYREEQGSGGYSLEIGSLAAVYRQKIEDNSQGKAFASKRVVPPSSSLDPVAPFPVLSVDPEYCVNPLAHPVFSVAQTDLQAFSMQNGLNGQVDVSLASAASAVESLKPALLAGQPLVYVPSASLFMLYGSLQEGPASGSGSERDDRSSEAPATVELSSAPSAQKRLCEERKPQEEDEPATKRQSREYEDGPLSLVMPKKPSDSTDLASPKTMGNRASIPLKDIHVNGQLPAAEEISGKATANSLVSSEWGNPSRNTDVEKPSKENESTKEPSLLQYLCVQSPA Predict reactive species |
| Full Name | E2F transcription factor 7 |
| Calculated Molecular Weight | 911 aa, 100 kDa |
| GenBank Accession Number | BC136366 |
| Gene Symbol | E2F7 |
| Gene ID (NCBI) | 144455 |
| RRID | AB_3670606 |
| Conjugate | Unconjugated |
| Purification Method | Protein A purification |
| UNIPROT ID | Q96AV8 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA. |
| FC protocol for E2F7 antibody 82887-1-RR | Download protocol |
| IF protocol for E2F7 antibody 82887-1-RR | Download protocol |
| Ekaitz (Verified Customer) (09-15-2025) | We tested this E2F7 antibody (82887-1-RR) in U2OS wild-type and E2F7 knockout cells at different dilutions. We observed the same nuclear signal in both WT and KO cells, suggesting that the detected signal is nonspecific. Applications: Immunofluorescence Primary Antibody Dilution: 1:400 Cell Tissue Type: U2OS E2F7 Antibody Immunofluorescence validation (1:400 dilution) in U2OS (Cat no:82887-1-RR) |
| Citations | - |
| Dilutions | IF/ICC : 1:500-1:2000 FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension |
| Isotype | IgG |
| Clonality | Recombinant |
| Clone Number | 230073C2 |
| Conjugation | Unconjugated |
E2F7 also named as E2F transcription factor 7 is a 911 amino acid protein, which belongs to the E2F/DP family. E2F7 localizes in the nucleus and mainly forms homodimers and, to a lesser extent, heterodimers with E2F8. E2F7 as a transcription factor involves in various processes such as angiogenesis, polyploidization of specialized cells and DNA damage response. E2F7 mainly forms homodimers and to a lesser extent, heterodimers with E2F8. (PMID: 18194653 ) Protocols Product Specific Protocols FC protocol for E2F7 antibody 82887-1-RR Download protocol IF protocol for E2F7 antibody 82887-1-RR Download protocol Standard Protocols Click here to view our Standard Protocols