ERF Polyclonal antibody 26468-1-AP proteintech
$149.00
In stock
SKU
26468-1-AP
| Catalog Number | 26468-1-AP |
| Synonyms | Ets2 repressor factor, PE 2, PE2 |
| Host | Rabbit |
| Reactivity | human, mouse |
| Form | Liquid |
| Formulation | PBS, Azide, Glycerol |
| Applications | WB, IF/ICC, IP, ELISA |
| Host / Isotype | Rabbit / IgG |
| Tested Applications — Positive WB detected in | A431 cells, A549 cells, J774A.1 cells, RAW 264.7 cells |
| Tested Applications — Positive IP detected in | A431 cells |
| Tested Applications — Positive IF/ICC detected in | MCF-7 cells |
| Recommended Dilutions — Western Blot (WB) | WB : 1:1000-1:4000 |
| Recommended Dilutions — Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Recommended Dilutions — Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| Positive WB detected in | A431 cells, A549 cells, J774A.1 cells, RAW 264.7 cells |
| Positive IP detected in | A431 cells |
| Positive IF/ICC detected in | MCF-7 cells |
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| Tested Reactivity | human, mouse |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | CatNo: Ag24189 Product name: Recombinant human ERF protein Source: e coli. -derived, PET28a Tag: 6*His Domain: 138-323 aa of BC022231 Sequence: GGSHFRFPPSTPSEVLSPTEDPRSPPACSSSSSSLFSAVVARRLGRGSVSDCSDGTSELEEPLGEDPRARPPGPPDLGAFRGPPLARLPHDPGVFRVYPRPRGGPEPLSPFPVSPLAGPGSLLPPQLSPALPMTPTHLAYTPSPTLSPMYPSGGGGPSGSGGGSHFSFSPEDMKRYLQAHTQSVYN Predict reactive species |
| Full Name | Ets2 repressor factor |
| Calculated Molecular Weight | 548 aa, 59 kDa |
| Observed Molecular Weight | 90 kDa |
| GenBank Accession Number | BC022231 |
| Gene Symbol | ERF |
| Gene ID (NCBI) | 2077 |
| RRID | AB_3669537 |
| Conjugate | Unconjugated |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P50548 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA. |
| IF protocol for ERF antibody 26468-1-AP | Download protocol |
| IP protocol for ERF antibody 26468-1-AP | Download protocol |
| WB protocol for ERF antibody 26468-1-AP | Download protocol |
| Citations | - |
| Dilutions | WB : 1:1000-1:4000 IP : 0.5-4.0 ug for IP and 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate for WB IF/ICC : 1:200-1:800 |
| Isotype | IgG |
| Clonality | Polyclonal |
| Clone Number | - |
| Conjugation | Unconjugated |
ERF (Ets2 repressor factor) is also named as PE-2. Erf is a gene for a ubiquitously expressed Ets DNA-binding domain-containing transcriptional repressor regulated by Erk-dependent phosphorylation (PMID: 28694332, PMID: 35258130). Erf is involved in T cell maturation, acting as a positive regulator during CD4 and eventually CD8 lineage commitment, while negatively regulates the production of γδ T cells (PMID: 35258130). Erf is required for both primitive and erythromyeloid progenitor waves of hematopoietic stem cell (HSC)-independent hematopoiesis as well as for the normal function of HSCs (PMID: 28694332). ETS2 repressor factor (ERF) insufficiency causes craniosynostosis (CRS4) in humans and mice. ERF is an ETS domain transcriptional repressor regulated by Erk1/2 phosphorylation via nucleo-cytoplasmic shuttling (PMID: 37175668). Protocols Product Specific Protocols IF protocol for ERF antibody 26468-1-AP Download protocol IP protocol for ERF antibody 26468-1-AP Download protocol WB protocol for ERF antibody 26468-1-AP Download protocol Standard Protocols Click here to view our Standard Protocols
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