CoraLite® Plus 488-conjugated ERO1L Monoclonal antibody CL488-67416 proteintech
$479.00
In stock
SKU
CL488-67416
| Catalog Number | CL488-67416 |
| Synonyms | ERO1 alpha, ERO1 L, ERO1 L alpha, ERO1 like (S. cerevisiae), ERO1 like protein alpha, ERO1L, Oxidoreductin 1 L alpha |
| Host | Mouse |
| Reactivity | Human, Mouse, Rat |
| Reactivity | human, mouse, rat |
| Form | Liquid |
| Formulation | PBS, Proclin300, BSA, Glycerol |
| Applications | IF-P |
| Host / Isotype | Mouse / IgG2b |
| Tested Applications — Positive IF-P detected in | human stomach cancer tissue |
| Recommended Dilutions — Immunofluorescence (IF)-P | IF-P : 1:200-1:800 |
| Positive IF-P detected in | human stomach cancer tissue |
| Immunofluorescence (IF)-P | IF-P : 1:200-1:800 |
| Tested Reactivity | Human, Mouse, Rat |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | CatNo: Ag29910 Product name: Recombinant human ERO1L protein Source: e coli. -derived, PET28a Tag: 6*His Domain: 20-390 aa of BC008674 Sequence: SGHGEEQPPETAAQRCFCQVSGYLDDCTCDVETIDRFNNYRLFPRLQKLLESDYFRYYKVNLKRPCPFWNDISQCGRRDCAVKPCQSDEVPDGIKSASYKYSEEANNLIEECEQAERLGAVDESLSEETQKAVLQWTKHDDSSDNFCEADDIQSPEAEYVDLLLNPERYTGYKGPDAWKIWNVIYEENCFKPQTIKRPLNPLASGQGTSEENTFYSWLEGLCVEKRAFYRLISGLHASINVHLSARYLLQETWLEKKWGHNITEFQQRFDGILTEGEGPRRLKNLYFLYLIELRALSKVLPFFERPDFQLFTGNKIQDEENKMLLLEILHEIKSFPLHFDENSFFAGDKKEAHKLKEDFRLHFRNISRIMD Predict reactive species |
| Full Name | ERO1-like (S. cerevisiae) |
| Calculated Molecular Weight | 468 aa, 54 kDa |
| Observed Molecular Weight | 54 kDa |
| GenBank Accession Number | BC008674 |
| Gene Symbol | ERO1L |
| Gene ID (NCBI) | 30001 |
| RRID | AB_2919467 |
| Conjugate | CoraLite® Plus 488 Fluorescent Dye |
| Excitation/Emission Maxima Wavelengths | 493 nm / 522 nm |
| Excitation Laser | Blue laser (488 nm) |
| Purification Method | Protein A purification |
| UNIPROT ID | Q96HE7 |
| Storage Buffer | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. |
| IF protocol for CL Plus 488 ERO1L antibody CL488-67416 | Download protocol |
| Citations | - |
| Dilutions | IF-P : 1:200-1:800 |
| Isotype | IgG2b |
| Clonality | Monoclonal |
| Clone Number | 1G12E11 |
| Conjugation | CoraLite® Plus 488 |
ERO1L, also named as ERO1-alpha, is an essential oxidoreductase that oxidizes proteins in the endoplasmic reticulum to produce disulfide bonds. It acts by oxidizing directly P4HB/PDI isomerase through a direct disulfide exchange. It does not act as a direct oxidant of folding substrate, but relies on P4HB/PDI to transfer oxidizing equivalent. Associates with ERP44 but not with GRP54, demonstrating that it does not oxidize all PDI related proteins and can discriminate between PDI and related proteins. Its reoxidation probably involves electron transfer to molecular oxygen via FAD. Glutathione may be required to regulate its activity in the endoplasmic reticulum. It may be responsible for a significant proportion of reactive oxygen species (ROS) in the cell, thereby being a source of oxidative stress. It is required for the folding of immunoglobulin proteins. Responsible for the release of the unfolded cholera toxin from reduced P4HB/PDI in case of infection by V.cholerae, thereby playing a role in retrotranslocation of the toxin. Protocols Product Specific Protocols IF protocol for CL Plus 488 ERO1L antibody CL488-67416 Download protocol Standard Protocols Click here to view our Standard Protocols
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