CoraLite® Plus 488-conjugated G3BP1 Monoclonal antibody CL488-66486 proteintech
$479.00
In stock
SKU
CL488-66486
| Catalog Number | CL488-66486 |
| Synonyms | G3BP, G3BP 1, G3BP1, HDH VIII |
| Host | Mouse |
| Reactivity | Human, mouse, rat, pig and More (1) |
| Reactivity | human, mouse, rat, pig |
| Form | Liquid |
| Formulation | PBS, Proclin300, BSA, Glycerol |
| Applications | IF/ICC |
| Host / Isotype | Mouse / IgG1 |
| Tested Applications — Positive IF/ICC detected in | sodium arsenite treated HeLa cells |
| Recommended Dilutions — Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| Positive IF/ICC detected in | sodium arsenite treated HeLa cells |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| Tested Reactivity | Human, mouse, rat, pig |
| Cited Reactivity | human |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | CatNo: Ag3728 Product name: Recombinant human G3BP1 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 167-466 aa of BC006997 Sequence: PDDSGTFYDQAVVSNDMEEHLEEPVAEPEPDPEPEPEQEPVSEIQEEKPEPVLEETAPEDAQKSSSPAPADIAQTVQEDLRTFSWASVTSKNLPPSGAVPVTGIPPHVVKVPASQPRPESKPESQIPPQRPQRDQRVREQRINIPPQRGPRPIREAGEQGDIEPRRMVRHPDSHQLFIGNLPHEVDKSELKDFFQSYGNVVELRINSGGKLPNFGFVVFDDSEPVQKVLSNRPIMFRGEVRLNVEEKKTRAAREGDRRDNRLRGPGGPRGGLGGGMRGPPRGGMVQKPGFGVGRGLAPRQ Predict reactive species |
| Full Name | GTPase activating protein (SH3 domain) binding protein 1 |
| Calculated Molecular Weight | 466 aa, 52 kDa |
| Observed Molecular Weight | 68 kDa |
| GenBank Accession Number | BC006997 |
| Gene Symbol | G3BP1 |
| Gene ID (NCBI) | 10146 |
| RRID | AB_2883345 |
| Conjugate | CoraLite® Plus 488 Fluorescent Dye |
| Excitation/Emission Maxima Wavelengths | 493 nm / 522 nm |
| Excitation Laser | Blue laser (488 nm) |
| Purification Method | Protein G purification |
| UNIPROT ID | Q13283 |
| Storage Buffer | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. |
| IF protocol for CL Plus 488 G3BP1 antibody CL488-66486 | Download protocol |
| human | IF Cell Death Dis Proximity proteomics reveals OTUD6B regulation of stress granule dynamics through coalescence with VCP/p97. Authors - Dian Yang View Article |
| Tatyana (Verified Customer) (01-21-2023) | Excellent bright signal only after 30 min incubation (1:1000) at RT. PFA-fixed cells were used. Labels sodium arsenite induced stress granules well. Applications: Immunofluorescence Primary Antibody Dilution: 1:1000 Cell Tissue Type: Human / U2OS cells CL Plus 488 G3BP1 Antibody Immunofluorescence validation (1:1000 dilution) in Human / U2OS cells (Cat no:CL488-66486) |
| Citations | 3 |
| Dilutions | IF/ICC : 1:50-1:500 |
| Isotype | IgG1 |
| Clonality | Monoclonal |
| Clone Number | 1E4A2 |
| Conjugation | CoraLite® Plus 488 |
GAP SH3 Binding Protein 1 (G3BP1), also named as G3BP, is an effector of stress granule (SG) assembly. SG biology plays an important role in the pathophysiology of TDP-43 in ALS and FTLD-U. G3BP1 can be used as a marker of SG. It has been shown to function downstream of Ras and play a role in RNA metabolism, signal transduction, and proliferation. G3BP1 is a ubiquitously expressed protein that localizes to the cytoplasm in proliferating cells and to the nucleus in non-proliferating cells. G3BP1 has recently been implicated in cancer biology. Protocols Product Specific Protocols IF protocol for CL Plus 488 G3BP1 antibody CL488-66486 Download protocol Standard Protocols Click here to view our Standard Protocols
Publications
- Stress granules restrain ferroptosis by sequestering ferritin
Journal: Nat Cell Biol | Authors: Zehe Ge - Tuning tRNA synthetase inhibition reveals parabolic induction of stress granules limited in size and RNA content.
Journal: RNA | Authors: Max Baymiller | Species: human | Application: IF - Proximity proteomics reveals OTUD6B regulation of stress granule dynamics through coalescence with VCP/p97.
Journal: Cell Death Dis | Authors: Dian Yang | Species: human | Application: IF
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