HLA-DRB5 Polyclonal antibody 21702-1-AP proteintech
$149.00
In stock
SKU
21702-1-AP
| Catalog Number | 21702-1-AP |
| Synonyms | DR beta 2, DR beta 5, DR beta-5, DR2-beta-2, Dw2 |
| Host | Rabbit |
| Reactivity | human, mouse, rat |
| Form | Liquid |
| Formulation | PBS, Azide, Glycerol |
| Applications | WB, IHC, IF-P, ELISA |
| Host / Isotype | Rabbit / IgG |
| Tested Applications — Positive WB detected in | Daudi cells, HeLa cells, HepG2 cells, HL-60 cells, Raji cells, Ramos cells |
| Tested Applications — Positive IHC detected in | human tonsillitis tissue, mouse spleen tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Tested Applications — Positive IF-P detected in | human tonsillitis tissue, mouse lung tissue, mouse spleen tissue |
| Recommended Dilutions — Western Blot (WB) | WB : 1:1000-1:4000 |
| Recommended Dilutions — Immunohistochemistry (IHC) | IHC : 1:200-1:800 |
| Recommended Dilutions — Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| Positive WB detected in | Daudi cells, HeLa cells, HepG2 cells, HL-60 cells, Raji cells, Ramos cells |
| Positive IHC detected in | human tonsillitis tissue, mouse spleen tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | human tonsillitis tissue, mouse lung tissue, mouse spleen tissue |
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunohistochemistry (IHC) | IHC : 1:200-1:800 |
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| Tested Reactivity | human, mouse, rat |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | CatNo: Ag16307 Product name: Recombinant human HLA-DRB5 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-266 aa of BC009234 Sequence: MVCLKLPGGSYMAKLTVTLMVLSSPLALAGDTRPRFLQQDKYECHFFNGTERVRFLHRDIYNQEEDLRFDSDVGEYRAVTELGRPDAEYWNSQKDFLEDRRAAVDTYCRHNYGVGESFTVQRRVEPKVTVYPARTQTLQHHNLLVCSVNGFYPGSIEVRWFRNSQEEKAGVVSTGLIQNGDWTFQTLVMLETVPRSGEVYTCQVEHPSVTSPLTVEWRAQSESAQSKMLSGVGGFVLGLLFLGAGLFIYFKNQKGHSGLHPTGLVS Predict reactive species |
| Full Name | major histocompatibility complex, class II, DR beta 5 |
| Calculated Molecular Weight | 266 aa, 30 kDa |
| Observed Molecular Weight | 30 kDa |
| GenBank Accession Number | BC009234 |
| Gene Symbol | HLA-DRB5 |
| Gene ID (NCBI) | 3127 |
| RRID | AB_2878905 |
| Conjugate | Unconjugated |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q30154 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA. |
| IF protocol for HLA-DRB5 antibody 21702-1-AP | Download protocol |
| IHC protocol for HLA-DRB5 antibody 21702-1-AP | Download protocol |
| WB protocol for HLA-DRB5 antibody 21702-1-AP | Download protocol |
| Sofia (Verified Customer) (02-03-2025) | Antibody works fine in IHC and IF, in tissue and cells. For Western blot doesn't work as well as in the other techniques. Applications: Western Blot, Immunohistochemistry, Immunofluorescence Primary Antibody Dilution: 1:500 Cell Tissue Type: brain and microglia |
| Citations | 1 |
| Dilutions | WB : 1:1000-1:4000 IHC : 1:200-1:800 IF-P : 1:50-1:500 |
| Isotype | IgG |
| Clonality | Polyclonal |
| Clone Number | - |
| Conjugation | Unconjugated |
Publications
- HLA-DRB5 promotes immune thrombocytopenia via activating CD8+ T cells
Journal: Open Med (Wars) | Authors: Qidong Ye | Application: WB
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