IVD Polyclonal antibody 10822-1-AP proteintech
$149.00
In stock
SKU
10822-1-AP
| Catalog Number | 10822-1-AP |
| Synonyms | Isovaleryl-CoA dehydrogenase, mitochondrial, EC:1.3.8.4, EC:1.3.8.1, Butyryl-CoA dehydrogenase, ACAD2 |
| Host | Rabbit |
| Reactivity | human, mouse, rat and More (1) |
| Reactivity | human, mouse, rat, arabidopsis |
| Form | Liquid |
| Formulation | PBS, Azide, Glycerol |
| Applications | WB, IHC, IF/ICC, IP, ELISA |
| Host / Isotype | Rabbit / IgG |
| Tested Applications — Positive WB detected in | MCF-7 cells, human brain tissue, mouse liver tissue, HepG2 cells, rat liver tissue |
| Tested Applications — Positive IP detected in | MCF-7 cells |
| Tested Applications — Positive IHC detected in | human liver cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Tested Applications — Positive IF/ICC detected in | MCF-7 cells |
| Recommended Dilutions — Western Blot (WB) | WB : 1:2000-1:12000 |
| Recommended Dilutions — Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Recommended Dilutions — Immunohistochemistry (IHC) | IHC : 1:300-1:1200 |
| Recommended Dilutions — Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| Positive WB detected in | MCF-7 cells, human brain tissue, mouse liver tissue, HepG2 cells, rat liver tissue |
| Positive IP detected in | MCF-7 cells |
| Positive IHC detected in | human liver cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | MCF-7 cells |
| Western Blot (WB) | WB : 1:2000-1:12000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:300-1:1200 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | mouse, rat, arabidopsis |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | CatNo: Ag1252 Product name: Recombinant human IVD protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-423 aa of BC017202 Sequence: MATATRLLGCRVASWRLRPPLAGFVSQRAHSLLPVDDAINGLSEEQRQLRQTMAKFLQEHLAPKAQEIDRSNEFKNLREFWKQLGNLGVLGITAPVQYGGSGLGYLEHVLVMEEISRASGAVGLSYGAHSNLCINQLVRNGNEAQKEKYLPKLISGEYIGALAMSEPNAGSDVVSMKLKAEKKGNHYILNGNKFWITNGPDADVLIVYAKTDLAAVPASRGITAFIVEKGMPGFSTSKKLDKLGMRGSNTCELIFEDCKIPAANILGHENKGVYVLMSGLDLERLVLAGGPLGLMQAVLDHTIPYLHVREAFGQKIGHFQLMQGKMADMYTRLMACRQYVYNVAKACDEGHCTAKDCAGVILYSAECATQVALDGIQCFGGNGYINDFPMGRFLRDAKLYEIGAGTSEVRRLVIGRAFNADFH Predict reactive species |
| Full Name | isovaleryl Coenzyme A dehydrogenase |
| Calculated Molecular Weight | 46 kDa |
| Observed Molecular Weight | 41-46 kDa |
| GenBank Accession Number | BC017202 |
| Gene Symbol | IVD |
| Gene ID (NCBI) | 3712 |
| RRID | AB_2296258 |
| Conjugate | Unconjugated |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P26440 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA. |
| IF protocol for IVD antibody 10822-1-AP | Download protocol |
| IHC protocol for IVD antibody 10822-1-AP | Download protocol |
| IP protocol for IVD antibody 10822-1-AP | Download protocol |
| WB protocol for IVD antibody 10822-1-AP | Download protocol |
| Arabidopsis | WB Plant J PMID: 19929878 Authors - Gu Liping L View Article |
| mouse | WB J Neurochem Proteomic analysis of stargazer mutant mouse neuronal proteins involved in absence seizure. Authors - Ryu Myung-Jeom MJ View Article |
| rat | WB Proteomics Clin Appl Loss of Pink1 modulates synaptic mitochondrial bioenergetics in the rat striatum prior to motor symptoms: concomitant complex I respiratory defects and increased complex II-mediated respiration. Authors - Kelly L Stauch View Article |
| Lana (Verified Customer) (06-21-2019) | Lines on WB:1 - BioRad Precision Plus Protein standards2 and 3 – biological replicates, lysates of mitochondrial fractions of ST-Hdh-Q7/Q7 mouse striatal cell line.SDS-PAGE:15 ug/ul RIPA lysate of the mitochondrial fraction of ST-Hdh-Q7/Q7 mouse striatal cell line4-12% Bis-tris gradient gelTransfer:Immobilon-FL transfer membranes (Millipore) O/N at 30V, 4CBlocking:SEA Block Blocking Buffer 1hPrimary Ab:O/N incubation at 4CSecondary Ab:IRDye 680LT Goat anti-Rabbit, 1h incubation at room temperature. Applications: Western Blot, Primary Antibody Dilution: 1:1000 Cell Tissue Type: ST-Hdh mouse striatal cell line IVD Antibody Western Blot, validation (1:1000 dilution) in ST-Hdh mouse striatal cell line (Cat no:10822-1-AP) |
| Citations | 8 |
| Dilutions | WB : 1:2000-1:12000 IP : 0.5-4.0 ug for IP and 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate for WB IHC : 1:300-1:1200 IF/ICC : 1:50-1:500 |
| Isotype | IgG |
| Clonality | Polyclonal |
| Clone Number | - |
| Conjugation | Unconjugated |
IVD(Isovaleryl CoA dehydrogenase, mitochondrial) is a member of the acylCoA dehydrogenase family and is involved in the catabolism of leucine. In size, IVD precursor and mature proteins produced by class I mutants are indistinguishable from their normal counterparts. Class II, III, and IV mutants make IVD precursor proteins 42 kD in size rather than the normal 45 kD(PMID:2063866). It has 2 isoforms produced by alternative splicing. Protocols Product Specific Protocols IF protocol for IVD antibody 10822-1-AP Download protocol IHC protocol for IVD antibody 10822-1-AP Download protocol IP protocol for IVD antibody 10822-1-AP Download protocol WB protocol for IVD antibody 10822-1-AP Download protocol Standard Protocols Click here to view our Standard Protocols
Publications
- PMID: 19929878
Journal: Plant J | Authors: Gu Liping L | Species: Arabidopsis | Application: WB - Mitochondrial FAD shortage in SLC25A32 deficiency affects folate-mediated one-carbon metabolism.
Journal: Cell Mol Life Sci | Authors: Min-Zhi Peng | Species: mouse | Application: WB - Research resource: A dual proteomic approach identifies regulated islet proteins during beta cell mass expansion in vivo.
Journal: Mol Endocrinol | Authors: Signe Horn | Species: mouse | Application: WB - Proteomic analysis of stargazer mutant mouse neuronal proteins involved in absence seizure.
Journal: J Neurochem | Authors: Ryu Myung-Jeom MJ | Species: mouse | Application: WB - The role of the electron-transfer flavoprotein: ubiquinone oxidoreductase following carbohydrate starvation in Arabidopsis cell cultures.
Journal: Plant Cell Rep | Authors: Danielle S Brito | Application: WB - Loss of Pink1 modulates synaptic mitochondrial bioenergetics in the rat striatum prior to motor symptoms: concomitant complex I respiratory defects and increased complex II-mediated respiration.
Journal: Proteomics Clin Appl | Authors: Kelly L Stauch | Species: rat | Application: WB - Research resource: A dual proteomic approach identifies regulated islet proteins during beta cell mass expansion in vivo.
Journal: Mol Endocrinol | Authors: Signe Horn | Species: mouse | Application: WB - Loss of Pink1 modulates synaptic mitochondrial bioenergetics in the rat striatum prior to motor symptoms: concomitant complex I respiratory defects and increased complex II-mediated respiration.
Journal: Proteomics Clin Appl | Authors: Kelly L Stauch | Species: rat | Application: WB
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