KLHL2 Recombinant monoclonal antibody 85893-1-RR proteintech
$149.00
In stock
SKU
85893-1-RR
| Catalog Number | 85893-1-RR |
| Synonyms | ABP KELCH, Actin-binding protein Mayven, Kelch-like protein 2 |
| Host | Rabbit |
| Reactivity | human, mouse, rat |
| Form | Liquid |
| Formulation | PBS, Azide, Glycerol |
| Applications | WB, IF/ICC, ELISA |
| Host / Isotype | Rabbit / IgG |
| Tested Applications — Positive WB detected in | mouse brain tissue, HEK-293T cells, A431 cells, MCF-7 cells, K-562 cells, rat brain tissue |
| Tested Applications — Positive IF/ICC detected in | MCF-7 cells |
| Recommended Dilutions — Western Blot (WB) | WB : 1:5000-1:50000 |
| Recommended Dilutions — Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| Positive WB detected in | mouse brain tissue, HEK-293T cells, A431 cells, MCF-7 cells, K-562 cells, rat brain tissue |
| Positive IF/ICC detected in | MCF-7 cells |
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| Tested Reactivity | human, mouse, rat |
| Class | Recombinant |
| Type | Antibody |
| Immunogen | CatNo: Ag5950 Product name: Recombinant human KLHL2 protein Source: e coli. -derived, PET28a Tag: 6*His Domain: 273-593 aa of BC022503 Sequence: DYLIEAMKYHLLPTEQRILMKSVRTRLRTPMNLPKLMVVVGGQAPKAIRSVECYDFKEERWHQVAELPSRRCRAGMVYMAGLVFAVGGFNGSLRVRTVDSYDPVKDQWTSVANMRDRRSTLGAAVLNGLLYAVGGFDGSTGLSSVEAYNIKSNEWFHVAPMNTRRSSVGVGVVGGLLYAVGGYDVASRQCLSTVECYNATTNEWTYIAEMSTRRSGAGVGVLNNLLYAVGGHDGPLVRKSVEVYDPTTNAWRQVADMNMCRRNAGVCAVNGLLYVVGGDDGSCNLASVEYYNPTTDKWTVVSSCMSTGRSYAGVTVIDKRL Predict reactive species |
| Full Name | kelch-like 2, Mayven (Drosophila) |
| Calculated Molecular Weight | 66 kDa |
| Observed Molecular Weight | 66 kDa |
| GenBank Accession Number | BC022503 |
| Gene Symbol | KLHL2 |
| Gene ID (NCBI) | 11275 |
| RRID | AB_3744452 |
| Conjugate | Unconjugated |
| Purification Method | Protein A purification |
| UNIPROT ID | O95198 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA. |
| IF protocol for KLHL2 antibody 85893-1-RR | Download protocol |
| WB protocol for KLHL2 antibody 85893-1-RR | Download protocol |
| Citations | - |
| Dilutions | WB : 1:5000-1:50000 IF/ICC : 1:200-1:800 |
| Isotype | IgG |
| Clonality | Recombinant |
| Clone Number | 250148G5 |
| Conjugation | Unconjugated |
KLHL2 contains an SH3-binding domain in the N terminus and a POZ domain and 6 tandem repeats of approximately 50 amino acids each in the C terminus. In astrocytoma/glioblastoma cells, KLHL2 colocalized with actin filaments in the cytoskeleton remodeling regions in stress fibers and in patchy cortical actin-rich regions of the cell margins. In primary rat hippocampal neurons, it was highly expressed in the cell body and in neurite processes(PMID: 10397770). Protocols Product Specific Protocols IF protocol for KLHL2 antibody 85893-1-RR Download protocol WB protocol for KLHL2 antibody 85893-1-RR Download protocol Standard Protocols Click here to view our Standard Protocols
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