Synaptotagmin-1 Monoclonal antibody 68043-1-Ig proteintech
$149.00
In stock
SKU
68043-1-Ig
| Catalog Number | 68043-1-Ig |
| Synonyms | Synaptotagmin 1, SYT1, 1H1H2, P65, SVP65 |
| Host | Mouse |
| Reactivity | human, mouse, rat, pig, rabbit, chicken |
| Form | Liquid |
| Formulation | PBS, Azide, Glycerol |
| Applications | WB, IHC, ELISA |
| Host / Isotype | Mouse / IgG1 |
| Tested Applications — Positive WB detected in | pig brain tissue, rat cerebellum tissue, mouse cerebellum tissue, rabbit brain tissue, rat brain tissue, mouse brain tissue, chicken brain tissue, rabbit cerebellum tissue |
| Tested Applications — Positive IHC detected in | mouse brain tissue, mouse cerebellum tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Recommended Dilutions — Western Blot (WB) | WB : 1:20000-1:100000 |
| Recommended Dilutions — Immunohistochemistry (IHC) | IHC : 1:2500-1:10000 |
| Positive WB detected in | pig brain tissue, rat cerebellum tissue, mouse cerebellum tissue, rabbit brain tissue, rat brain tissue, mouse brain tissue, chicken brain tissue, rabbit cerebellum tissue |
| Positive IHC detected in | mouse brain tissue, mouse cerebellum tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Western Blot (WB) | WB : 1:20000-1:100000 |
| Immunohistochemistry (IHC) | IHC : 1:2500-1:10000 |
| Tested Reactivity | human, mouse, rat, pig, rabbit, chicken |
| Cited Reactivity | human |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | CatNo: Ag6071 Product name: Recombinant human SYT1 protein Source: e coli. -derived, PET28a Tag: 6*His Domain: 97-422 aa of BC058917 Sequence: GGKNAINMKDVKDLGKTMKDQALKDDDAETGLTDGEEKEEPKEEEKLGKLQYSLDYDFQNNQLLVGIIQAAELPALDMGGTSDPYVKVFLLPDKKKKFETKVHRKTLNPVFNEQFTFKVPYSELGGKTLVMAVYDFDRFSKHDIIGEFKVPMNTVDFGHVTEEWRDLQSAEKEEQEKLGDICFSLRYVPTAGKLTVVILEAKNLKKMDVGGLSDPYVKIHLMQNGKRLKKKKTTIKKNTLNPYYNESFSFEVPFEQIQKVQVVVTVLDYDKIGKNDAIGKVFVGYNSTGAELRHWSDMLANPRRPIAQWHTLQVEEEVDAMLAVKK Predict reactive species |
| Full Name | synaptotagmin I |
| Calculated Molecular Weight | 48 kDa |
| Observed Molecular Weight | 60-65 kDa |
| GenBank Accession Number | BC058917 |
| Gene Symbol | Synaptotagmin-1 |
| Gene ID (NCBI) | 6857 |
| RRID | AB_2918785 |
| Conjugate | Unconjugated |
| Purification Method | Protein G purification |
| UNIPROT ID | P21579 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA. |
| IHC protocol for Synaptotagmin-1 antibody 68043-1-Ig | Download protocol |
| WB protocol for Synaptotagmin-1 antibody 68043-1-Ig | Download protocol |
| human | WB F1000Res A guide to selecting high-performing antibodies for Synaptotagmin-1 (Uniprot ID P21579) for use in western blot, immunoprecipitation, immunofluorescence and flow cytometry Authors - Michael S Biddle View Article |
| Citations | 1 |
| Dilutions | WB : 1:20000-1:100000 IHC : 1:2500-1:10000 |
| Isotype | IgG1 |
| Clonality | Monoclonal |
| Clone Number | 1H1H2 |
| Conjugation | Unconjugated |
The synaptotagmins are integral membrane proteins of synaptic vesicles thought to serve as Ca(2+) sensors in the process of vesicular trafficking and exocytosis (PMID: 8058779). SYT1 (synaptotagmin I) was firstly identified as a 65-kDa protein with a wide distribution in neuronal and neurosecretory tissue (PMID: 7298720). Calcium binding to SYT1 participates in triggering neurotransmitter release at the synapse (PMID: 11242035). In addition to regulating exocytosis, SYT1 has also been implicated in endocytosis and neurite outgrowth (PMID: 15492212; 11078930; 10336114). Protocols Product Specific Protocols IHC protocol for Synaptotagmin-1 antibody 68043-1-Ig Download protocol WB protocol for Synaptotagmin-1 antibody 68043-1-Ig Download protocol Standard Protocols Click here to view our Standard Protocols
Publications
- A guide to selecting high-performing antibodies for Synaptotagmin-1 (Uniprot ID P21579) for use in western blot, immunoprecipitation, immunofluorescence and flow cytometry
Journal: F1000Res | Authors: Michael S Biddle | Species: human | Application: WB
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