L1TD1 Polyclonal antibody 21528-1-AP proteintech
$149.00
In stock
SKU
21528-1-AP
| Catalog Number | 21528-1-AP |
| Synonyms | ECAT11, ES cell associated protein 11, ES cell-associated protein 11, LINE-1 type transposase domain-containing protein 1 |
| Host | Rabbit |
| Reactivity | human and More (1) |
| Reactivity | human, mouse |
| Form | Liquid |
| Formulation | PBS, Azide, Glycerol |
| Applications | WB, IHC, IF/ICC, IP, RIP, ELISA |
| Applications | WB, IHC, IF/ICC, ELISA |
| Host / Isotype | Rabbit / IgG |
| Tested Applications — Positive WB detected in | NCCIT cells |
| Tested Applications — Positive IHC detected in | human ovary cancer tissue, human ovary tumor tissue, human placenta tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Tested Applications — Positive IF/ICC detected in | NCCIT cells |
| Recommended Dilutions — Western Blot (WB) | WB : 1:500-1:1000 |
| Recommended Dilutions — Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| Recommended Dilutions — Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| Positive WB detected in | NCCIT cells |
| Positive IHC detected in | human ovary cancer tissue, human ovary tumor tissue, human placenta tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | NCCIT cells |
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| Tested Reactivity | human |
| Cited Reactivity | mouse |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | CatNo: Ag16147 Product name: Recombinant human L1TD1 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 522-865 aa of BC111559 Sequence: KLVKHQVVHKTQEEEETAVPTSQGTGTPCLTLCLASPSKSLEMSHDEHKKHSHTNLSISTGVTKLKKTEEKKHRTLHTEELTSKEADLTEETEENLRSSVINSIREIKEEIGNLKSSHSGVLEIENSVDDLSSRMDILEERIDSLEDQIEEFSKDTMQMTKQIISKERQRDIEERSRSCNIRLIGIPEKESYENRAEDIIKEIIDENFAELKKGSSLEIVSACRVPSKIDEKRLTPRHILVKFWNSSDKEKIIRASRERREITYQGTRIRLTADLSLDTLDARSKWSNVFKVLLEKGFNPRILYPAKMAFDFRGKTKVFLSIEEFRDYVLHMPTLRELLGNNIP Predict reactive species |
| Full Name | LINE-1 type transposase domain containing 1 |
| Calculated Molecular Weight | 865 aa, 99 kDa |
| Observed Molecular Weight | 100 kDa |
| GenBank Accession Number | BC111559 |
| Gene Symbol | L1TD1 |
| Gene ID (NCBI) | 54596 |
| RRID | AB_2878878 |
| Conjugate | Unconjugated |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q5T7N2 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA. |
| IHC protocol for L1TD1 antibody 21528-1-AP | Download protocol |
| IF protocol for L1TD1 antibody 21528-1-AP | Download protocol |
| mouse | WB,RIP,IP Nat Commun Microprotein PLUM encoded by Lin28b uORF is a cytoplasmic determinant of pluripotency and embryonic development Authors - Zhihong Hao View Article |
| Citations | 2 |
| Dilutions | WB : 1:500-1:1000 IHC : 1:500-1:2000 IF/ICC : 1:50-1:500 |
| Isotype | IgG |
| Clonality | Polyclonal |
| Clone Number | - |
| Conjugation | Unconjugated |
L1TD1 is an RNA-binding protein highly and specifically expressed in pluripotent cells. It is essential for maintaining the pluripotentency in human embryonic stem cells (hESCs) and has been considered as a marker for undifferentiated hESCs. L1TD1 is also reported to be highly expressed in medulloblastoma cells and its expression in medulloblastoma correlates with poor clinical outcome. Protocols Product Specific Protocols IHC protocol for L1TD1 antibody 21528-1-AP Download protocol IF protocol for L1TD1 antibody 21528-1-AP Download protocol Standard Protocols Click here to view our Standard Protocols
Publications
- Microprotein PLUM encoded by Lin28b uORF is a cytoplasmic determinant of pluripotency and embryonic development
Journal: Nat Commun | Authors: Zhihong Hao | Species: mouse | Application: WB,RIP,IP - Comprehensive Profiling of N(6)-methyladnosine (m(6)A) Readouts Reveals Novel m(6)A Readers That Regulate Human Embryonic Stem Cell Differentiation.
Journal: Adv Sci (Weinh) | Authors: Zhou Huang
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