LAD1 Polyclonal antibody 16136-1-AP proteintech
$149.00
In stock
SKU
16136-1-AP
| Catalog Number | 16136-1-AP |
| Synonyms | LAD, Lad 1, LAD1, LadA, ladinin 1, Linear IgA disease antigen |
| Host | Rabbit |
| Reactivity | human, mouse |
| Form | Liquid |
| Formulation | PBS, Azide, Glycerol |
| Applications | WB, IF, IHC, ELISA |
| Applications | WB, IHC, ELISA |
| Host / Isotype | Rabbit / IgG |
| Tested Applications — Positive WB detected in | MCF-7 cells |
| Tested Applications — Positive IHC detected in | human colon cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Recommended Dilutions — Western Blot (WB) | WB : 1:2000-1:16000 |
| Recommended Dilutions — Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| Positive WB detected in | MCF-7 cells |
| Positive IHC detected in | human colon cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Western Blot (WB) | WB : 1:2000-1:16000 |
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| Tested Reactivity | human, mouse |
| Cited Reactivity | human |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | CatNo: Ag9159 Product name: Recombinant human LAD1 protein Source: e coli. -derived, PET28a Tag: 6*His Domain: 168-517 aa of BC009742 Sequence: ERKKGVPEKSPVLEKSSMPKKTAPEKSLVSDKTSISEKVLASEKTSLSEKIAVSEKRNSSEKKSVLEKTSVSEKSLAPGMALGSGRRLVSEKASIFEKALASEKSPTADAKPAPKRATASEQPLAQEPPASGGSPATTKEQRGRALPGKNLPSLAKQGASDPPTVASRLPPVTLQVKIPSKEEEADMSSPTQRTYSSSLKRSSPRTISFRMKPKKENSETTLTRSASMKLPDNTVKLGEKLERYHTAIRRSESVKSRGLPCTELFVAPVGVASKRHLFEKELAGQSRAEPASSRKENLRLSGVVTSRLNLWISRTQESGDQDPQEAQKASSATERTQWGQKSDSSLDAEV Predict reactive species |
| Full Name | ladinin 1 |
| Calculated Molecular Weight | 517 aa, 57 kDa |
| Observed Molecular Weight | 57 kDa |
| GenBank Accession Number | BC009742 |
| Gene Symbol | LAD1 |
| Gene ID (NCBI) | 3898 |
| RRID | AB_2081705 |
| Conjugate | Unconjugated |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | O00515 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA. |
| IHC protocol for LAD1 antibody 16136-1-AP | Download protocol |
| WB protocol for LAD1 antibody 16136-1-AP | Download protocol |
| human | WB,IHC,IF J Transl Med LAD1 promotes malignant progression by diminishing ubiquitin-dependent degradation of vimentin in gastric cancer Authors - Yingming Jiang View Article |
| Citations | 1 |
| Dilutions | WB : 1:2000-1:16000 IHC : 1:500-1:2000 |
| Isotype | IgG |
| Clonality | Polyclonal |
| Clone Number | - |
| Conjugation | Unconjugated |
Ladinin-1, also named as LADA, or LAD1, is an anchoring filament protein which is a component of the basement membrane zone. It is a novel component of the basement membranes and may function in contributing to the stability of the association of the epithelial layers with the underlying mesenchyme. Human ladinin is an autoantigen associated with linear IgA disease. It is a secreted protein. Immunohistochemical staining of 16136-1-AP shows strong extracellular matrixc positivity in human testis. The predicted MW of this protein is 57 kDa. Protocols Product Specific Protocols IHC protocol for LAD1 antibody 16136-1-AP Download protocol WB protocol for LAD1 antibody 16136-1-AP Download protocol Standard Protocols Click here to view our Standard Protocols
Publications
- LAD1 promotes malignant progression by diminishing ubiquitin-dependent degradation of vimentin in gastric cancer
Journal: J Transl Med | Authors: Yingming Jiang | Species: human | Application: WB,IHC,IF
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