NGEF Polyclonal antibody 13271-1-AP proteintech
$149.00
In stock
SKU
13271-1-AP
| Catalog Number | 13271-1-AP |
| Synonyms | EPHEXIN, Ephexin 1, Ephexin-1, NGEF |
| Host | Rabbit |
| Reactivity | human, mouse, rat |
| Form | Liquid |
| Formulation | PBS, Azide, Glycerol |
| Applications | WB, IHC, IP, ELISA |
| Applications | WB, IP, IHC, ELISA |
| Host / Isotype | Rabbit / IgG |
| Tested Applications — Positive WB detected in | mouse brain tissue, mouse testis tissue, rat brain tissue |
| Tested Applications — Positive IP detected in | mouse brain tissue |
| Tested Applications — Positive IHC detected in | human brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Recommended Dilutions — Western Blot (WB) | WB : 1:1000-1:8000 |
| Recommended Dilutions — Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Recommended Dilutions — Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Positive WB detected in | mouse brain tissue, mouse testis tissue, rat brain tissue |
| Positive IP detected in | mouse brain tissue |
| Positive IHC detected in | human brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Western Blot (WB) | WB : 1:1000-1:8000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | rat |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | CatNo: Ag3967 Product name: Recombinant human NGEF protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-273 aa of BC031573 Sequence: METRESEDLEKTRRKSASDQWNTDNEPAKVKPELLPEKEETSQADQDIQDKEPHCHIPIKRNSIFNRSIRRKSKAKARDNPERNASCLADSQDNGKSVNEPLTLNIPWSRTPPCRTAMQTDPGAQEMSESSSTPGNGATPEEWPALADSPTTLTEALRMIHPIPADSWRNLIEQIGLLYQEYRDKSTLQEIETRRQQDAEIEDNTNGSPASEDTPEEEEEEEEEEEPASPPERKTLPQICLLSNPHSRFNLWQDLPEIRSSGVLEILQPEEIK Predict reactive species |
| Full Name | neuronal guanine nucleotide exchange factor |
| Calculated Molecular Weight | 710 aa, 82 kDa |
| Observed Molecular Weight | 71 kDa |
| GenBank Accession Number | BC031573 |
| Gene Symbol | NGEF |
| Gene ID (NCBI) | 25791 |
| RRID | AB_2152381 |
| Conjugate | Unconjugated |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q8N5V2 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA. |
| IHC protocol for NGEF antibody 13271-1-AP | Download protocol |
| IP protocol for NGEF antibody 13271-1-AP | Download protocol |
| WB protocol for NGEF antibody 13271-1-AP | Download protocol |
| rat | WB Brain Res Bull Electroacupuncture ameliorates chronic unpredictable mild stress-induced depression-like behavior and cognitive impairment through suppressing oxidative stress and neuroinflammation in rats Authors - Tao Tong View Article |
| Citations | 2 |
| Dilutions | WB : 1:1000-1:8000 IP : 0.5-4.0 ug for IP and 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate for WB IHC : 1:50-1:500 |
| Isotype | IgG |
| Clonality | Polyclonal |
| Clone Number | - |
| Conjugation | Unconjugated |
Publications
- Up-regulation of NGEF via the BRAFV600E /ERK/AP1 pathway enhances invasion and migration abilities of BRAFV600E-mutant thyroid cancer
Journal: Biochem Biophys Rep | Authors: Zhao Fu - Electroacupuncture ameliorates chronic unpredictable mild stress-induced depression-like behavior and cognitive impairment through suppressing oxidative stress and neuroinflammation in rats
Journal: Brain Res Bull | Authors: Tao Tong | Species: rat | Application: WB
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