PACT Polyclonal antibody 10771-1-AP proteintech
$149.00
In stock
SKU
10771-1-AP
| Catalog Number | 10771-1-AP |
| Synonyms | DYT16, HSD 14, HSD14, PACT, PKR associated protein X, PKR associating protein X, PRKRA, RAX |
| Host | Rabbit |
| Reactivity | human, mouse, rat |
| Form | Liquid |
| Formulation | PBS, Azide, Glycerol |
| Applications | WB, IHC, IF, CoIP, ELISA |
| Applications | WB, IHC, ELISA |
| Host / Isotype | Rabbit / IgG |
| Tested Applications — Positive WB detected in | HeLa cells, MCF-7 cells, C2C12 cells, PC-12 cells |
| Tested Applications — Positive IHC detected in | mouse testis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Recommended Dilutions — Western Blot (WB) | WB : 1:500-1:3000 |
| Recommended Dilutions — Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Positive WB detected in | HeLa cells, MCF-7 cells, C2C12 cells, PC-12 cells |
| Positive IHC detected in | mouse testis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Western Blot (WB) | WB : 1:500-1:3000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | CatNo: Ag1214 Product name: Recombinant human PRKRA protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-313 aa of BC009470 Sequence: MSQSRHRAEAPPLEREDSGTFSLGKMITAKPGKTPIQVLHEYGMKTKNIPVYECERSDVQIHVPTFTFRVTVGDITCTGEGTSKKLAKHRAAEAAINILKANASICFAVPDPLMPDPSKQPKNQLNPIGSLQELAIHHGWRLPEYTLSQEGGPAHKREYTTICRLESFMETGKGASKKQAKRNAAEKFLAKFSNISPENHISLTNVVGHSLGCTWHSLRNSPGEKINLLKRSLLSIPNTDYIQLLSEIAKEQGFNITYLDIDELSANGQYQCLAELSTSPITVCHGSGISCGNAQSDAAHNALQYLKIIAERK Predict reactive species |
| Full Name | protein kinase, interferon-inducible double stranded RNA dependent activator |
| Calculated Molecular Weight | 34 kDa |
| Observed Molecular Weight | 31-34 kDa |
| GenBank Accession Number | BC009470 |
| Gene Symbol | PRKRA |
| Gene ID (NCBI) | 8575 |
| RRID | AB_2170496 |
| Conjugate | Unconjugated |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | O75569 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA. |
| IHC protocol for PACT antibody 10771-1-AP | Download protocol |
| WB protocol for PACT antibody 10771-1-AP | Download protocol |
| human | WB J Proteome Res Proteomic analysis of early HIV-1 nucleoprotein complexes. Authors - Schweitzer Cameron J CJ View Article |
| rat | IF EMBO Rep The dynamic recruitment of TRBP to neuronal membranes mediates dendritogenesis during development. Authors - Anna Antoniou View Article |
| Hannah (Verified Customer) (02-18-2025) | single band of expected size Applications: Western Blot Primary Antibody Dilution: 1/1000 Cell Tissue Type: human fibroblasts PACT Antibody Western Blot validation (1/1000 dilution) in human fibroblasts (Cat no:10771-1-AP) |
| Citations | 16 |
| Dilutions | WB : 1:500-1:3000 IHC : 1:50-1:500 |
| Isotype | IgG |
| Clonality | Polyclonal |
| Clone Number | - |
| Conjugation | Unconjugated |
PRKRA (PKR-associated protein X) is also named as PACT, RAX, HSD-14 and HSD14. PRKRA was significantly overexpressed in PC tissues and promoted the proliferation, migration, and chemoresistance of PC cells (PMID: 37484321). PRKRA activates EIF2AK2/PKR in the absence of double-stranded RNA (dsRNA), leading to phosphorylation of EIF2S1/EFI2-alpha and inhibition of translation and induction of apoptosis. PRKRA has been shown to interact with DICER1. DICER1 and PRKRA expression in normal colon was limited to mucosal epithelium and showed similar cytoplasmic expression (PMID: 37484321). Protocols Product Specific Protocols IHC protocol for PACT antibody 10771-1-AP Download protocol WB protocol for PACT antibody 10771-1-AP Download protocol Standard Protocols Click here to view our Standard Protocols
Publications
- Prkra dimer senses double-stranded RNAs to dictate global translation efficiency
Journal: Mol Cell | Authors: Tong Lu - LINC00921 reduces lung cancer radiosensitivity by destabilizing NUDT21 and driving aberrant MED23 alternative polyadenylation
Journal: Cell Rep | Authors: Nasha Zhang | Species: human | Application: WB - The dynamic recruitment of TRBP to neuronal membranes mediates dendritogenesis during development.
Journal: EMBO Rep | Authors: Anna Antoniou | Species: rat | Application: IF - Hepato-specific microRNA-122 facilitates accumulation of newly synthesized miRNA through regulating PRKRA.
Journal: Nucleic Acids Res | Authors: Li Shuai S | Species: human | Application: WB - KSHV inhibits stress granule formation by viral ORF57 blocking PKR activation.
Journal: PLoS Pathog | Authors: Nishi R Sharma | Species: human | Application: WB - Proteomic analysis of early HIV-1 nucleoprotein complexes.
Journal: J Proteome Res | Authors: Schweitzer Cameron J CJ | Species: human | Application: WB
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