RAB31-specific Polyclonal antibody 16182-1-AP proteintech
$149.00
In stock
SKU
16182-1-AP
| Catalog Number | 16182-1-AP |
| Synonyms | RAB31, RAB22B, Ras related protein Rab 31, Ras-related protein Rab-22B, Ras-related protein Rab-31 |
| Host | Rabbit |
| Reactivity | human, mouse, rat |
| Form | Liquid |
| Formulation | PBS, Azide, Glycerol |
| Applications | WB, IHC, IF, IP, CoIP, ELISA |
| Applications | WB, IHC, IP, ELISA |
| Host / Isotype | Rabbit / IgG |
| Tested Applications — Positive WB detected in | rat brain tissue, HeLa cells, human brain tissue, K-562 cells |
| Tested Applications — Positive IP detected in | U-87 MG cells |
| Tested Applications — Positive IHC detected in | human colon tissue, human heart tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Recommended Dilutions — Western Blot (WB) | WB : 1:500-1:3000 |
| Recommended Dilutions — Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Recommended Dilutions — Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Positive WB detected in | rat brain tissue, HeLa cells, human brain tissue, K-562 cells |
| Positive IP detected in | U-87 MG cells |
| Positive IHC detected in | human colon tissue, human heart tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Western Blot (WB) | WB : 1:500-1:3000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Peptide Predict reactive species |
| Full Name | RAB31, member RAS oncogene family |
| Calculated Molecular Weight | 22 kDa |
| Observed Molecular Weight | 22 kDa |
| GenBank Accession Number | NM_006868 |
| Gene Symbol | RAB31 |
| Gene ID (NCBI) | 11031 |
| RRID | AB_1851568 |
| Conjugate | Unconjugated |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q13636 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA. |
| IHC protocol for RAB31-specific antibody 16182-1-AP | Download protocol |
| IP protocol for RAB31-specific antibody 16182-1-AP | Download protocol |
| WB protocol for RAB31-specific antibody 16182-1-AP | Download protocol |
| human | WB,IHC Life Sci Upregulation of Rab31 is associated with poor prognosis and promotes colorectal carcinoma proliferation via the mTOR/p70S6K/Cyclin D1 signalling pathway. Authors - Li Yang KD Validated View Article |
| mouse,human | WB,IHC,IF Int J Biochem Cell Biol Rab31 promotes activation of hepatic stellate cells by accelerating TGF-β receptor II complex endocytosis. Authors - Chan Qiu KD Validated View Article |
| Edward (Verified Customer) (11-15-2022) | Showed up reasonably well using 12 ug protein lysate with fluorescent secondary Applications: Western Blot Primary Antibody Dilution: 1:500 Cell Tissue Type: K562, KCL22 and THP-1 |
| Citations | 12 |
| Dilutions | WB : 1:500-1:3000 IP : 0.5-4.0 ug for IP and 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate for WB IHC : 1:50-1:500 |
| Isotype | IgG |
| Clonality | Polyclonal |
| Clone Number | - |
| Conjugation | Unconjugated |
RAB 31, also named as RAB22b, belongs to small GTP-binding proteins of the RAB family. Rab31 localizes in the trans-Golgi network (TGN) and endosomes. It is required for transport of mannose 6-phosphate receptors (MPRs) from the trans-Golgi network (TGN) to endosomes. This antibody can bind RAB31 specifically, and not react RAB22a. Protocols Product Specific Protocols IHC protocol for RAB31-specific antibody 16182-1-AP Download protocol IP protocol for RAB31-specific antibody 16182-1-AP Download protocol WB protocol for RAB31-specific antibody 16182-1-AP Download protocol Standard Protocols Click here to view our Standard Protocols
Publications
- RAB31 marks and controls an ESCRT-independent exosome pathway.
Journal: Cell Res | Authors: Denghui Wei KD Validated | Species: human | Application: WB,IF - Rab31 promotes the invasion and metastasis of cervical cancer cells by inhibiting MAPK6 degradation.
Journal: Int J Biol Sci | Authors: Yujie Huang KD Validated | Species: human | Application: WB - RAB31 Targeted by MiR-30c-2-3p Regulates the GLI1 Signaling Pathway, Affecting Gastric Cancer Cell Proliferation and Apoptosis.
Journal: Front Oncol | Authors: Chao-Tao Tang KD Validated | Species: human | Application: WB,IHC,CoIP - Human liver cell trafficking mutants: characterization and whole exome sequencing.
Journal: PLoS One | Authors: Fei Yuan | Species: human | Application: WB - Rab31 promotes activation of hepatic stellate cells by accelerating TGF-β receptor II complex endocytosis.
Journal: Int J Biochem Cell Biol | Authors: Chan Qiu KD Validated | Species: mouse,human | Application: WB,IHC,IF - Upregulation of Rab31 is associated with poor prognosis and promotes colorectal carcinoma proliferation via the mTOR/p70S6K/Cyclin D1 signalling pathway.
Journal: Life Sci | Authors: Li Yang KD Validated | Species: human | Application: WB,IHC - Overexpression of RAB31 in gastric cancer is associated with released exosomes and increased tumor cell invasion and metastasis
Journal: Cancer Med | Authors: Shan Wu | Species: human | Application: WB,IHC - Rab31 promotes activation of hepatic stellate cells by accelerating TGF-β receptor II complex endocytosis.
Journal: Int J Biochem Cell Biol | Authors: Chan Qiu | Species: mouse,human | Application: WB,IHC,IF - RAB31 Targeted by MiR-30c-2-3p Regulates the GLI1 Signaling Pathway, Affecting Gastric Cancer Cell Proliferation and Apoptosis.
Journal: Front Oncol | Authors: Chao-Tao Tang | Species: human | Application: WB,IHC,CoIP - Association between Rab31/rs9965664 polymorphism and immunoglobulin therapy resistance in patients with Kawasaki disease
Journal: Front Cardiovasc Med | Authors: Hongyan Yu | Species: human | Application: WB - Human liver cell trafficking mutants: characterization and whole exome sequencing.
Journal: PLoS One | Authors: Fei Yuan | Species: human | Application: WB - RAB31 in glioma-derived endothelial cells promotes glioma cell invasion via extracellular vesicle-mediated enrichment of MYO1C
Journal: FEBS Open Bio | Authors: Jinghao Suo | Species: human | Application: WB
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