RABGAP1L Polyclonal antibody 13894-1-AP proteintech
$149.00
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SKU
13894-1-AP
| Catalog Number | 13894-1-AP |
| Synonyms | Rab GTPase-activating protein 1-like, isoform 10 |
| Host | Rabbit |
| Reactivity | human, mouse, rat |
| Form | Liquid |
| Formulation | PBS, Azide, Glycerol |
| Applications | WB, IHC, IF/ICC, IP, ELISA |
| Host / Isotype | Rabbit / IgG |
| Tested Applications — Positive WB detected in | HeLa cells, MCF-7 cells |
| Tested Applications — Positive IP detected in | HeLa cells |
| Tested Applications — Positive IHC detected in | human hepatocirrhosis tissue, human placenta tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Tested Applications — Positive IF/ICC detected in | Hela cells |
| Recommended Dilutions — Western Blot (WB) | WB : 1:500-1:2000 |
| Recommended Dilutions — Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Recommended Dilutions — Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Recommended Dilutions — Immunofluorescence (IF)/ICC | IF/ICC : 1:10-1:100 |
| Positive WB detected in | HeLa cells, MCF-7 cells |
| Positive IP detected in | HeLa cells |
| Positive IHC detected in | human hepatocirrhosis tissue, human placenta tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | Hela cells |
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:10-1:100 |
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | CatNo: Ag4856 Product name: Recombinant human RABGAP1L protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-355 aa of BC041888 Sequence: MEVRASLQKIVSNGDEQLEKAMEEILRDSEKRPSSLLVDCQSSSEISDHSFGDIPASQTNKPSLQLILDPSNTEISTPRPSSPGGLPEEDSVLFNKLTYLGCMKVSSPRNEVEALRAMATMKSSSQYPFPVTLYVPNVPEGSVRIIDQSSNVEIASFPIYKVLFCARGHDGTTESNCFAFTESSHGSEEFQIHVFSCEIKEAVSRILYSFCTAFKRSSRQVSDVKDSVIPTPDSDVFTFSVSLEVKEDDGKGNFSPVPKDRDKFYFKLKQGIEKKVVITVQQLSNKELAIERCFGMLLSPGRNVKNSDMHLLDMESMGKSYDGRAYVITGMWNPNAPVFLALNEETPKDKQVYMT Predict reactive species |
| Full Name | RAB GTPase activating protein 1-like |
| Calculated Molecular Weight | 815 aa, 93 kDa |
| Observed Molecular Weight | 93-105 kDa |
| GenBank Accession Number | BC041888 |
| Gene Symbol | RABGAP1L |
| Gene ID (NCBI) | 9910 |
| RRID | AB_10638308 |
| Conjugate | Unconjugated |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q5R372 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA. |
| IF protocol for RABGAP1L antibody 13894-1-AP | Download protocol |
| IHC protocol for RABGAP1L antibody 13894-1-AP | Download protocol |
| IP protocol for RABGAP1L antibody 13894-1-AP | Download protocol |
| WB protocol for RABGAP1L antibody 13894-1-AP | Download protocol |
| human | WB,IF Cell Rep Restriction factor screening identifies RABGAP1L-mediated disruption of endocytosis as a host antiviral defense. Authors - Sonja Fernbach View Article |
| rat | IHC BMC Pregnancy Childbirth Core biomarkers analysis benefit for diagnosis on human intrahepatic cholestasis of pregnancy Authors - Yan Fang View Article |
| mouse | WB J Cell Biol TBC1D18 is a Rab5-GAP that coordinates endosome maturation together with Mon1 Authors - Shu Hiragi KD Validated View Article |
| Citations | 5 |
| Dilutions | WB : 1:500-1:2000 IP : 0.5-4.0 ug for IP and 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate for WB IHC : 1:20-1:200 IF/ICC : 1:10-1:100 |
| Isotype | IgG |
| Clonality | Polyclonal |
| Clone Number | - |
| Conjugation | Unconjugated |
RABGAP1L, also known as TBC1D18, is a member of the Tre2/Bub2/Cdc16 (TBC)-domain-containing protein family. Overexpression of RABGAP1L potentiated the antiviral action of IFN against a panel of viruses, including IAV, and its function required a catalytically active TBC domain. Protocols Product Specific Protocols IF protocol for RABGAP1L antibody 13894-1-AP Download protocol IHC protocol for RABGAP1L antibody 13894-1-AP Download protocol IP protocol for RABGAP1L antibody 13894-1-AP Download protocol WB protocol for RABGAP1L antibody 13894-1-AP Download protocol Standard Protocols Click here to view our Standard Protocols
Publications
- The oncogene AAMDC links PI3K-AKT-mTOR signaling with metabolic reprograming in estrogen receptor-positive breast cancer.
Journal: Nat Commun | Authors: Emily Golden | Species: human | Application: IHC - Restriction factor screening identifies RABGAP1L-mediated disruption of endocytosis as a host antiviral defense.
Journal: Cell Rep | Authors: Sonja Fernbach | Species: human | Application: WB,IF - RabGAP1L modulates Rab7A and Rab10 to orchestrate cell-autonomous immunity
Journal: Cell Rep | Authors: Atsuko Minowa-Nozawa - Core biomarkers analysis benefit for diagnosis on human intrahepatic cholestasis of pregnancy
Journal: BMC Pregnancy Childbirth | Authors: Yan Fang | Species: rat | Application: IHC - TBC1D18 is a Rab5-GAP that coordinates endosome maturation together with Mon1
Journal: J Cell Biol | Authors: Shu Hiragi KD Validated | Species: mouse | Application: WB
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