RAI14 Polyclonal antibody 17507-1-AP proteintech
$149.00
In stock
SKU
17507-1-AP
| Catalog Number | 17507-1-AP |
| Synonyms | retinoic acid induced 14, RAI13, NORPEG, KIAA1334, Ankyrin repeat and coiled-coil structure-containing protein |
| Host | Rabbit |
| Reactivity | human, mouse, rat |
| Form | Liquid |
| Formulation | PBS, Azide, Glycerol |
| Applications | WB, IHC, IF/ICC, IP, ELISA |
| Host / Isotype | Rabbit / IgG |
| Tested Applications — Positive WB detected in | HeLa cells, human placenta tissue, mouse testis tissue |
| Tested Applications — Positive IP detected in | mouse testis tissue |
| Tested Applications — Positive IHC detected in | human stomach cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Tested Applications — Positive IF/ICC detected in | Hela cells |
| Recommended Dilutions — Western Blot (WB) | WB : 1:500-1:2000 |
| Recommended Dilutions — Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Recommended Dilutions — Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Recommended Dilutions — Immunofluorescence (IF)/ICC | IF/ICC : 1:10-1:100 |
| Positive WB detected in | HeLa cells, human placenta tissue, mouse testis tissue |
| Positive IP detected in | mouse testis tissue |
| Positive IHC detected in | human stomach cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | Hela cells |
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:10-1:100 |
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | CatNo: Ag11708 Product name: Recombinant human RAI14 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-347 aa of BC052988 Sequence: MKSLKAKFRKSDTNEWNKNDDRLLQAVENGDAEKVASLLGKKGASATKHDSEGKTAFHLAAAKGHVECLRVMITHGVDVTAQDTTGHSALHLAAKNSHHECIRKLLQSKCPAESVDSSGKTALHYAAAQGCLQAVQILCEHKSPINLKDLDGNIPLLLAVQNGHSEICHFLLDHGADVNSRNKSGRTALMLACEIGSSNAVEALIKKGADLNLVDSLGYNALHYSKLSENAGIQSLLLSKISQDADLKTPTKPKQHDQVSKISSERSGTPKKRKAPPPPISPTQLSDVSSPRSITSTPLSGKESVFFAEPPFKAEISSIRENKDRLSDSTTGADSLLDISSEADQQD Predict reactive species |
| Full Name | retinoic acid induced 14 |
| Calculated Molecular Weight | 980 aa, 110 kDa |
| Observed Molecular Weight | 110 kDa |
| GenBank Accession Number | BC052988 |
| Gene Symbol | RAI14 |
| Gene ID (NCBI) | 26064 |
| RRID | AB_2175992 |
| Conjugate | Unconjugated |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9P0K7 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA. |
| IF protocol for RAI14 antibody 17507-1-AP | Download protocol |
| IHC protocol for RAI14 antibody 17507-1-AP | Download protocol |
| IP protocol for RAI14 antibody 17507-1-AP | Download protocol |
| WB protocol for RAI14 antibody 17507-1-AP | Download protocol |
| human,mouse | WB,IP Elife Retinoic acid-induced protein 14 controls dendritic spine dynamics associated with depressive-like behaviors. Authors - Soo Jeong Kim View Article |
| mouse | WB,IF Cells Cysteine-Rich LIM-Only Protein 4 (CRP4) Promotes Atherogenesis in the ApoE-/- Mouse Model. Authors - Natalie Längst View Article |
| Human | WB Front Pharmacol High Expression of RAI14 in Triple-Negative Breast Cancer Participates in Immune Recruitment and Implies Poor Prognosis Through Bioinformatics Analyses. Authors - Ranliang Cui View Article |
| human | WB Cell Transplant RAI14 Regulated by circNFATC3/miR-23b-3p axis Facilitates Cell Growth and Invasion in Gastric Cancer. Authors - XinXin Yan View Article |
| Citations | 12 |
| Dilutions | WB : 1:500-1:2000 IP : 0.5-4.0 ug for IP and 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate for WB IHC : 1:50-1:500 IF/ICC : 1:10-1:100 |
| Isotype | IgG |
| Clonality | Polyclonal |
| Clone Number | - |
| Conjugation | Unconjugated |
Publications
- Localization of Kif1c mRNA to cell protrusions dictates binding partner specificity of the encoded protein
Journal: Genes Dev | Authors: Megan L Norris | Application: WB - Retinoic acid-induced protein 14 links mechanical forces to Hippo signaling
Journal: EMBO Rep | Authors: Wonyoung Jeong | Application: IF - Retinoic acid-induced protein 14 controls dendritic spine dynamics associated with depressive-like behaviors.
Journal: Elife | Authors: Soo Jeong Kim | Species: human,mouse | Application: WB,IP - Cysteine-Rich LIM-Only Protein 4 (CRP4) Promotes Atherogenesis in the ApoE-/- Mouse Model.
Journal: Cells | Authors: Natalie Längst | Species: mouse | Application: WB,IF - High Expression of RAI14 in Triple-Negative Breast Cancer Participates in Immune Recruitment and Implies Poor Prognosis Through Bioinformatics Analyses.
Journal: Front Pharmacol | Authors: Ranliang Cui | Species: Human | Application: WB - RAI14 Regulated by circNFATC3/miR-23b-3p axis Facilitates Cell Growth and Invasion in Gastric Cancer.
Journal: Cell Transplant | Authors: XinXin Yan | Species: human | Application: WB - Renal tubular-derived retinoic acid drives renal fibrosis via a RAI14-TRIOBP-YAP mechanotransduction axis.
Journal: Cell Signal | Authors: Jun Zhu | Species: mouse, rat, human | Application: WB - RAI14 Regulated by circNFATC3/miR-23b-3p axis Facilitates Cell Growth and Invasion in Gastric Cancer.
Journal: Cell Transplant | Authors: XinXin Yan | Species: human | Application: WB - Retinoic Acid Induced Protein 14 (Rai14) is dispensable for mouse spermatogenesis.
Journal: PeerJ | Authors: Yangyang Wu | Species: mouse | Application: WB,IF - Rai14 (retinoic acid induced protein 14) is involved in regulating f-actin dynamics at the ectoplasmic specialization in the rat testis*.
Journal: PLoS One | Authors: Xiaojing Qian | Species: rat | Application: WB,IF,IP - Knockdown of RAI14 suppresses the progression of gastric cancer.
Journal: Onco Targets Ther | Authors: Cheng Chen | Species: human | Application: WB - Retinoic acid-induced protein 14 controls dendritic spine dynamics associated with depressive-like behaviors.
Journal: Elife | Authors: Soo Jeong Kim | Species: human,mouse | Application: WB,IP
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