RIF1 Polyclonal antibody 30119-1-AP proteintech
$149.00
In stock
SKU
30119-1-AP
| Catalog Number | 30119-1-AP |
| Synonyms | DKFZp434D1026, DKFZp781N1478, FLJ12870, RIF1 |
| Host | Rabbit |
| Reactivity | Human, mouse and More (1) |
| Reactivity | human, mouse |
| Form | Liquid |
| Formulation | PBS, Azide, Glycerol |
| Applications | WB, IHC, IF/ICC, ELISA |
| Host / Isotype | Rabbit / IgG |
| Tested Applications — Positive WB detected in | A431 cells, HEK-293T cells, HT-29 cells, HeLa cells, HepG2 cells, MCF-7 cells |
| Tested Applications — Positive IHC detected in | human cervical cancer tissue, mouse testis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Tested Applications — Positive IF/ICC detected in | HeLa cells, HEK-293 cells |
| Recommended Dilutions — Western Blot (WB) | WB : 1:1000-1:4000 |
| Recommended Dilutions — Immunohistochemistry (IHC) | IHC : 1:400-1:1600 |
| Recommended Dilutions — Immunofluorescence (IF)/ICC | IF/ICC : 1:600-1:2400 |
| Positive WB detected in | A431 cells, HEK-293T cells, HT-29 cells, HeLa cells, HepG2 cells, MCF-7 cells |
| Positive IHC detected in | human cervical cancer tissue, mouse testis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells, HEK-293 cells |
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunohistochemistry (IHC) | IHC : 1:400-1:1600 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:600-1:2400 |
| Tested Reactivity | Human, mouse |
| Cited Reactivity | human |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | CatNo: Ag32422 Product name: Recombinant human RIF1 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 2277-2472 aa of NM_001177663.1 Sequence: SEMAKESIPCPTESVYPPLVNCVAPVDIILPQITSNMWARGLGQLIRAKNIKTIGDLSTLTASEIKTLPIRSPKVSNVKKALRIYHEQQVKTRGLEEIPVFDISEKTVNGIENKSLSPDEERLVSDIIDPVALEIPLSKNLLAQISALALQLDSEDLHNYSGSQLFEMHEKLSCMANSVIKNLQSRWRSPSHENSI Predict reactive species |
| Full Name | RAP1 interacting factor homolog (yeast) |
| Calculated Molecular Weight | 274 kDa |
| Observed Molecular Weight | 300 kDa |
| GenBank Accession Number | NM_001177663.1 |
| Gene Symbol | RIF1 |
| Gene ID (NCBI) | 55183 |
| RRID | AB_2935516 |
| Conjugate | Unconjugated |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q5UIP0 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA. |
| IF protocol for RIF1 antibody 30119-1-AP | Download protocol |
| IHC protocol for RIF1 antibody 30119-1-AP | Download protocol |
| WB protocol for RIF1 antibody 30119-1-AP | Download protocol |
| human | WB,IF Mol Cancer ACOX2 destabilizes the MRE11-RAD50-NBS1 complex and boosts anticancer immunity via the cGAS-STING pathway in clear cell renal cell carcinoma. Authors - Shiqi Ye View Article |
| Citations | 1 |
| Dilutions | WB : 1:1000-1:4000 IHC : 1:400-1:1600 IF/ICC : 1:600-1:2400 |
| Isotype | IgG |
| Clonality | Polyclonal |
| Clone Number | - |
| Conjugation | Unconjugated |
RIF1 (Rap1-interacting factor 1) is a multifunctional chromosome stability protein implicated in controlling DNA replication and repair. Human RIF1 can interact with protein phosphatase 1 (PP1) and the interaction is crucial for controlling DNA replication by limiting phosphorylation of the MCM complex (PMID: 28077461). RIF1 also acts with PP1 to protect nascent DNA from overdegradation at stalled replication forks (PMID: 31141682). RIF1 promotes non-homologous end joining (NHEJ) in double strand break (DSB) repair (PMID: 30443189). Research studies show that RIF1 is overexpressed in breast cancer tissues and is upgraded in NSCLC tissues (PMID: 30237512). Protocols Product Specific Protocols IF protocol for RIF1 antibody 30119-1-AP Download protocol IHC protocol for RIF1 antibody 30119-1-AP Download protocol WB protocol for RIF1 antibody 30119-1-AP Download protocol Standard Protocols Click here to view our Standard Protocols
Publications
- ACOX2 destabilizes the MRE11-RAD50-NBS1 complex and boosts anticancer immunity via the cGAS-STING pathway in clear cell renal cell carcinoma.
Journal: Mol Cancer | Authors: Shiqi Ye | Species: human | Application: WB,IF
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