RNF114 Polyclonal antibody 14338-1-AP proteintech
$149.00
In stock
SKU
14338-1-AP
| Catalog Number | 14338-1-AP |
| Synonyms | E3 ubiquitin-protein ligase RNF114, EC:2.3.2.27, PSORS12, RING-type E3 ubiquitin transferase RNF114, ZNF228 |
| Host | Rabbit |
| Reactivity | human, mouse |
| Form | Liquid |
| Formulation | PBS, Azide, Glycerol |
| Applications | WB, IHC, IF/ICC, ELISA |
| Host / Isotype | Rabbit / IgG |
| Tested Applications — Positive WB detected in | Jurkat cells, MCF-7 cells |
| Tested Applications — Positive IHC detected in | human ovary cancer tissue, mouse testis tissue, human testis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Tested Applications — Positive IF/ICC detected in | BGC-823 cells |
| Recommended Dilutions — Western Blot (WB) | WB : 1:1000-1:8000 |
| Recommended Dilutions — Immunohistochemistry (IHC) | IHC : 1:300-1:1200 |
| Recommended Dilutions — Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| Positive WB detected in | Jurkat cells, MCF-7 cells |
| Positive IHC detected in | human ovary cancer tissue, mouse testis tissue, human testis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | BGC-823 cells |
| Western Blot (WB) | WB : 1:1000-1:8000 |
| Immunohistochemistry (IHC) | IHC : 1:300-1:1200 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| Tested Reactivity | human, mouse |
| Cited Reactivity | mouse |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | CatNo: Ag5649 Product name: Recombinant human RNF114 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-228 aa of BC066919 Sequence: MAAQQRDCGGAAQLAGPAAEADPLGRFTCPVCLEVYEKPVQVPCGHVFCSACLQECLKPKKPVCGVCRSALAPGVRAVELERQIESTETSCHGCRKNFFLSKIRSHVATCSKYQNYIMEGVKATIKDASLQPRNVPNRYTFPCPYCPEKNFDQEGLVEHCKLFHSTDTKSVVCPICASMPWGDPNYRSANFREHIQRRHRFSYDTFVDYDVDEEDMMNQVLQRSIIDQ Predict reactive species |
| Full Name | ring finger protein 114 |
| Calculated Molecular Weight | 26 kDa |
| Observed Molecular Weight | 26 kDa |
| GenBank Accession Number | BC066919 |
| Gene Symbol | RNF114 |
| Gene ID (NCBI) | 55905 |
| RRID | AB_3085435 |
| Conjugate | Unconjugated |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9Y508 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA. |
| IF protocol for RNF114 antibody 14338-1-AP | Download protocol |
| IHC protocol for RNF114 antibody 14338-1-AP | Download protocol |
| WB protocol for RNF114 antibody 14338-1-AP | Download protocol |
| mouse | WB bioRxiv NAD + Sensing by PARP7 Regulates the C/EBPβ-Dependent Transcription Program in Adipose Tissue In Vivo Authors - MiKayla S Stokes KD Validated View Article |
| Citations | 3 |
| Dilutions | WB : 1:1000-1:8000 IHC : 1:300-1:1200 IF/ICC : 1:200-1:800 |
| Isotype | IgG |
| Clonality | Polyclonal |
| Clone Number | - |
| Conjugation | Unconjugated |
RNF114, also known as ZNF228 or ZNF313, is a member of the zinc-binding protein family. RNF114 contains two C2H2 and Ring finger zinc-binding domains and a ubiquitin-interacting motif (UIM). RNF114 functions as an E3 ligase in the ubiquitin-proteasome system and plays an important role in basic cellular processes in different types of cells. RNF114 is expressed widely in many normal tissues, such as the heart, liver, and kidney. RNF114 has 2 isoforms with the molecular mass of 21 and 26 kDa. RNF114 is a novel determinant of psoriasis susceptibility, and its overexpression is a crucial indicator of epithelial inflammation (PMID: 34104941, 35069903, 37523804). Protocols Product Specific Protocols IF protocol for RNF114 antibody 14338-1-AP Download protocol IHC protocol for RNF114 antibody 14338-1-AP Download protocol WB protocol for RNF114 antibody 14338-1-AP Download protocol Standard Protocols Click here to view our Standard Protocols
Publications
- BOULE is Essential for the Dynamic Disassembly of Heat Shock Granules in Male Germ Cells.
Journal: Adv Sci (Weinh) | Authors: Xin Li - NAD(+) sensing by PARP7 regulates the C/EBPβ-dependent transcription program during adipogenesis.
Journal: Cell Rep | Authors: MiKayla S. Stokes KD Validated | Species: mouse | Application: WB - NAD + Sensing by PARP7 Regulates the C/EBPβ-Dependent Transcription Program in Adipose Tissue In Vivo
Journal: bioRxiv | Authors: MiKayla S Stokes KD Validated | Species: mouse | Application: WB
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