CoraLite®555-conjugated RPA2 Monoclonal antibody CL555-67999 proteintech
$479.00
In stock
SKU
CL555-67999
| Catalog Number | CL555-67999 |
| Synonyms | RP A p32, RF-A protein 2, replication protein A2, 32kDa, Replication factor A protein 2, REPA2 |
| Host | Mouse |
| Reactivity | human, mouse, rat, pig |
| Form | Liquid |
| Formulation | PBS, Proclin300, BSA, Glycerol |
| Applications | IF/ICC, FC (Intra) |
| Host / Isotype | Mouse / IgG1 |
| Tested Applications — Positive IF/ICC detected in | A431 cells |
| Tested Applications — Positive FC (Intra) detected in | HeLa cells |
| Recommended Dilutions — Immunofluorescence (IF)/ICC | IF/ICC : 1:500-1:2000 |
| Recommended Dilutions — Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
| Positive IF/ICC detected in | A431 cells |
| Positive FC (Intra) detected in | HeLa cells |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:500-1:2000 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
| Tested Reactivity | human, mouse, rat, pig |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | CatNo: Ag24783 Product name: Recombinant human RPA2 protein Source: e coli. -derived, PET28a Tag: 6*His Domain: 80-270 aa of BC001630 Sequence: IRHAEKAPTNIVYKIDDMTAAPMDVRQWVDTDDTSSENTVVPPETYVKVAGHLRSFQNKKSLVAFKIMPLEDMNEFTTHILEVINAHMVLSKANSQPSAGRAPISNPGMSEAGNFGGNSFMPANGLTVAQNQVLNLIKACPRPEGLNFQDLKNQLKHMSVSSIKQAVDFLSNEGHIYSTVDDDHFKSTDAE Predict reactive species |
| Full Name | replication protein A2, 32kDa |
| Calculated Molecular Weight | 29 kDa |
| Observed Molecular Weight | 30 kDa |
| GenBank Accession Number | BC001630 |
| Gene Symbol | RPA2 |
| Gene ID (NCBI) | 6118 |
| RRID | AB_2934688 |
| Conjugate | CoraLite®555 Fluorescent Dye |
| Excitation/Emission Maxima Wavelengths | 557 nm / 570 nm |
| Excitation Laser | Green Laser (532 nm), Yellow-Green Laser (561 nm) |
| Purification Method | Protein G purification |
| UNIPROT ID | P15927 |
| Storage Buffer | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. |
| FC protocol for CL555 RPA2 antibody CL555-67999 | Download protocol |
| IF protocol for CL555 RPA2 antibody CL555-67999 | Download protocol |
| Citations | - |
| Dilutions | IF/ICC : 1:500-1:2000 FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
| Isotype | IgG1 |
| Clonality | Monoclonal |
| Clone Number | 1B9H11 |
| Conjugation | CoraLite®555 |
RPA2, a subunit of the heterotrimeric replication protein A (RPA) complex, is overexpressed in various cancers. RPA2 is a major target for upstream kinases, including CDKs and the three PIKKs (ATR, ataxia-telangiectasia mutated [ATM], and DNA-PK). RPA2 comprises multiple critical Ser/Thr residues that are phosphorylated in sequential order in response to genotoxic stress. Protocols Product Specific Protocols FC protocol for CL555 RPA2 antibody CL555-67999 Download protocol IF protocol for CL555 RPA2 antibody CL555-67999 Download protocol Standard Protocols Click here to view our Standard Protocols
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