RanGAP1 Polyclonal antibody 27405-1-AP proteintech
$149.00
In stock
SKU
27405-1-AP
| Catalog Number | 27405-1-AP |
| Synonyms | KIAA1835, Ran GTPase-activating protein 1, SD |
| Host | Rabbit |
| Reactivity | human, mouse |
| Form | Liquid |
| Formulation | PBS, Azide, Glycerol |
| Applications | WB, IHC, IF, ELISA |
| Applications | WB, IHC, ELISA |
| Host / Isotype | Rabbit / IgG |
| Tested Applications — Positive WB detected in | HEK-293 cells, MCF-7 cells, Neuro-2a cells, SKOV-3 cells, NIH/3T3 cells |
| Tested Applications — Positive IHC detected in | human stomach tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Recommended Dilutions — Western Blot (WB) | WB : 1:500-1:2000 |
| Recommended Dilutions — Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Positive WB detected in | HEK-293 cells, MCF-7 cells, Neuro-2a cells, SKOV-3 cells, NIH/3T3 cells |
| Positive IHC detected in | human stomach tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | CatNo: Ag26128 Product name: Recombinant human RANGAP1 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 2-220 aa of BC014044 Sequence: ASEDIAKLAETLAKTQVAGGQLSFKGKSLKLNTAEDAKDVIKEIEDFDSLEALRLEGNTVGVEAARVIAKALEKKSELKRCHWSDMFTGRLRTEIPPALISLGEGLITAGAQLVELDLSDNAFGPDGVQGFEALLKSSACFTLQELKLNNCGMGIGGGKILAAALTECHRKSSAQGKPLALKVFVAGRNRLENDGATALAEAFRVIGTLEEVHMPQNGI Predict reactive species |
| Full Name | Ran GTPase activating protein 1 |
| Calculated Molecular Weight | 64 kDa |
| Observed Molecular Weight | 64 kDa, 82 kDa |
| GenBank Accession Number | BC014044 |
| Gene Symbol | RANGAP1 |
| Gene ID (NCBI) | 5905 |
| RRID | AB_2880863 |
| Conjugate | Unconjugated |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P46060 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA. |
| IHC protocol for RanGAP1 antibody 27405-1-AP | Download protocol |
| WB protocol for RanGAP1 antibody 27405-1-AP | Download protocol |
| human | WB Cell Death Dis SERPINB3 enhances NPM1 sumoylation via inhibiting SENP3's activity and promotes lung tumorigenesis. Authors - Xiangyu Meng View Article |
| mouse | WB,IF iScience Characterizing the differential distribution and targets of Sumo1 and Sumo2 in the mouse brain Authors - Terry R Suk View Article |
| Citations | 3 |
| Dilutions | WB : 1:500-1:2000 IHC : 1:50-1:500 |
| Isotype | IgG |
| Clonality | Polyclonal |
| Clone Number | - |
| Conjugation | Unconjugated |
RanGAP1 is a protein that associates with the nuclear pore complex and participates in the regulation of nuclear transport. In mammalian cells, unmodified RanGAP1 is predominantly cytoplasmic, whereas modification by small ubiquitin-related modifier protein (SUMO) targets RanGAP1 to the cytoplasmic filaments of nuclear pore complex (NPC) where it forms a complex with RanBP2 and Ubc9. Phosphorylation of RanGAP1 occurs in a cell-cycle-dependent manner and may play a role in regulating RanGAP1 catalytic activity. We got 64 kDa (cytoplasmic Ran GAP1) and 82 kDa (SUMO-1 modified Ran GAP1) in western blotting. Protocols Product Specific Protocols IHC protocol for RanGAP1 antibody 27405-1-AP Download protocol WB protocol for RanGAP1 antibody 27405-1-AP Download protocol Standard Protocols Click here to view our Standard Protocols
Publications
- An ARF6-Exportin-5 axis delivers pre-miRNA cargo to tumour microvesicles.
Journal: Nat Cell Biol | Authors: James W Clancy | Species: human | Application: WB - Characterizing the differential distribution and targets of Sumo1 and Sumo2 in the mouse brain
Journal: iScience | Authors: Terry R Suk | Species: mouse | Application: WB,IF - SERPINB3 enhances NPM1 sumoylation via inhibiting SENP3's activity and promotes lung tumorigenesis.
Journal: Cell Death Dis | Authors: Xiangyu Meng | Species: human | Application: WB
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