AIF Recombinant monoclonal antibody 86156-1-RR proteintech
$149.00
In stock
SKU
86156-1-RR
| Catalog Number | 86156-1-RR |
| Synonyms | AIFM1, Apoptosis-inducing factor 1, mitochondrial, EC:1.6.99.-, PDCD8 |
| Host | Rabbit |
| Reactivity | human, mouse, rat |
| Form | Liquid |
| Formulation | PBS, Azide, Glycerol |
| Applications | WB, IF/ICC, ELISA |
| Host / Isotype | Rabbit / IgG |
| Tested Applications — Positive WB detected in | HeLa cells, HEK-293T cells, Jurkat cells, K-562 cells, NIH/3T3 cells, C2C12 cells, C6 cells |
| Tested Applications — Positive IF/ICC detected in | HeLa cells |
| Recommended Dilutions — Western Blot (WB) | WB : 1:5000-1:50000 |
| Recommended Dilutions — Immunofluorescence (IF)/ICC | IF/ICC : 1:500-1:2000 |
| Positive WB detected in | HeLa cells, HEK-293T cells, Jurkat cells, K-562 cells, NIH/3T3 cells, C2C12 cells, C6 cells |
| Positive IF/ICC detected in | HeLa cells |
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:500-1:2000 |
| Tested Reactivity | human, mouse, rat |
| Class | Recombinant |
| Type | Antibody |
| Immunogen | CatNo: Ag13268 Product name: Recombinant human AIF protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 259-609 aa of BC111065 Sequence: TPRSLSAIDRAGAEVKSRTTLFRKIGDFRSLEKISREVKSITIIGGGFLGSELACALGRKARALGTEVIQLFPEKGNMGKILPEYLSNWTMEKVRREGVKVMPNAIVQSVGVSSGKLLIKLKDGRKVETDHIVAAVGLEPNVELAKTGGLEIDSDFGGFRVNAELQARSNIWVAGDAACFYDIKLGRRRVEHHDHAVVSGRLAGENMTGAAKPYWHQSMFWSDLGPDVGYEAIGLVDSSLPTVGVFAKATAQDNPKSATEQSGTGIRSESETESEASEITIPPSTPAVPQAPVQGEDYGKGVIFYLRDKVVVGIVLWNIFNRMPIARKIIKDGEQHEDLNEVAKLFNIHED Predict reactive species |
| Full Name | apoptosis-inducing factor, mitochondrion-associated, 1 |
| Calculated Molecular Weight | 609 aa, 66 kDa |
| Observed Molecular Weight | 67 kDa |
| GenBank Accession Number | BC111065 |
| Gene Symbol | AIF |
| Gene ID (NCBI) | 9131 |
| RRID | AB_3744668 |
| Conjugate | Unconjugated |
| Purification Method | Protein A purification |
| UNIPROT ID | O95831 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA. |
| IF protocol for AIF antibody 86156-1-RR | Download protocol |
| WB protocol for AIF antibody 86156-1-RR | Download protocol |
| Citations | - |
| Dilutions | WB : 1:5000-1:50000 IF/ICC : 1:500-1:2000 |
| Isotype | IgG |
| Clonality | Recombinant |
| Clone Number | 250772G11 |
| Conjugation | Unconjugated |
Apoptosis-inducing factor (AIF) is one of the mitochondrial proteins to be released into the cytosol during apoptosis, and it is discovered as the first protein that regulates caspase-independent apoptosis(PMID:20494118). AIF is encoded as a 67 kDa protein that contains a mitochondrial localization signal (MLS) in the N-terminus.It is cleaved from the 62 kDa to the 57 kDa form following ischemic injury and translocated from the mitochondria to the nucleus in a calpain-dependent manner(PMID:19332058). Protocols Product Specific Protocols IF protocol for AIF antibody 86156-1-RR Download protocol WB protocol for AIF antibody 86156-1-RR Download protocol Standard Protocols Click here to view our Standard Protocols
Reviews
Write Your Own Review
Related Products
Check items to add to the cart or
