AKD2 Polyclonal antibody 27554-1-AP proteintech
$149.00
In stock
SKU
27554-1-AP
| Catalog Number | 27554-1-AP |
| Synonyms | AK9, Adenylate kinase domain-containing protein 1, Adenylate kinase domain-containing protein 2, AK 9, AKD1 |
| Host | Rabbit |
| Reactivity | human |
| Form | Liquid |
| Formulation | PBS, Azide, Glycerol |
| Applications | WB, IHC, ELISA |
| Host / Isotype | Rabbit / IgG |
| Tested Applications — Positive WB detected in | SMMC-7721 cells, HepG2 cells, HuH-7 cells |
| Tested Applications — Positive IHC detected in | human heart tissue, human liver cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Recommended Dilutions — Western Blot (WB) | WB : 1:1000-1:4000 |
| Recommended Dilutions — Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Positive WB detected in | SMMC-7721 cells, HepG2 cells, HuH-7 cells |
| Positive IHC detected in | human heart tissue, human liver cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Tested Reactivity | human |
| Cited Reactivity | human |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | CatNo: Ag25805 Product name: Recombinant human AKD2 protein Source: e coli. -derived, PET28a Tag: 6*His Domain: 1-421 aa of BC022031 Sequence: MTSQEKTEEYPFADIFDEDETERNFLLSKPVCFVVFGKPGVGKTTLARYITQAWKCIRVEALPILEEQIAAETESGVMLQSMLISGQSIPDELVIKLMLEKLNSPEVCHFGYIITEIPSLSQDAMTTLQQIELIKNLNLKPDVIINIKCPDYDLCQRISGQRQHNNTGYIYSRDQWDPEVIENHRKKKKEAQKDGKGEEEEEEEEQEEEEAFIAEMQMVAEILHHLVQRPEDYLENVENIVKLYKETILQTLEEVMAEHNPQYLIELNGNKPAEELFMIVMDRLKYLNLKRAAILTKLQGAEEEINDTMENDELFRTLASYKLIAPRYRWQRSKWGRTCPVNLKDGNIYSGLPDYSVSFLGKIYCLSSEEALKPFLLNPRPYLLPPMPGPPCKVFILGPQYSGKTTLCNMLAENYKGKVTN Predict reactive species |
| Full Name | adenylate kinase domain containing 2 |
| Calculated Molecular Weight | 221 kDa |
| Observed Molecular Weight | 45-50 kDa |
| GenBank Accession Number | BC022031 |
| Gene Symbol | AKD2 |
| Gene ID (NCBI) | 221264 |
| RRID | AB_2880904 |
| Conjugate | Unconjugated |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q5TCS8 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA. |
| IHC protocol for AKD2 antibody 27554-1-AP | Download protocol |
| WB protocol for AKD2 antibody 27554-1-AP | Download protocol |
| human | WB Mol Carcinog Integrative analyses of N6-methyladenosine-associated single-nucleotide polymorphisms (m6A-SNPs) identify tumor suppressor gene AK9 in lung cancer Authors - Tingting Hua View Article |
| Citations | 1 |
| Dilutions | WB : 1:1000-1:4000 IHC : 1:50-1:500 |
| Isotype | IgG |
| Clonality | Polyclonal |
| Clone Number | - |
| Conjugation | Unconjugated |
AK9, also named as adenylate kinase 9, belongs to adenylate kinase family, which is a small, ususlly monomeric, enzyme found in every living thing due to its crucial role in energenic metabolism(PMID: 30666486). AK9 is a nucleoside mono- and diphosphate kinase due to its broad phosphatase activity, the local concentration of substrates probably influences catalytic activity(PMID: 24495878). This antibody can detect 45-50 kDa band of AK9(PMID: 23416111). Protocols Product Specific Protocols IHC protocol for AKD2 antibody 27554-1-AP Download protocol WB protocol for AKD2 antibody 27554-1-AP Download protocol Standard Protocols Click here to view our Standard Protocols
Publications
- Integrative analyses of N6-methyladenosine-associated single-nucleotide polymorphisms (m6A-SNPs) identify tumor suppressor gene AK9 in lung cancer
Journal: Mol Carcinog | Authors: Tingting Hua | Species: human | Application: WB
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