SNRNP200 Polyclonal antibody 23875-1-AP proteintech
$149.00
In stock
SKU
23875-1-AP
| Catalog Number | 23875-1-AP |
| Synonyms | Activating signal cointegrator 1 complex subunit 3-like 1, ASCC3L1, BRR2, EC:3.6.4.13, HELIC2 |
| Host | Rabbit |
| Reactivity | human, mouse |
| Form | Liquid |
| Formulation | PBS, Azide, Glycerol |
| Applications | WB, IHC, IF, FC (Intra), IP, CoIP, RIP, ELISA |
| Applications | WB, IHC, FC (Intra), IP, ELISA |
| Host / Isotype | Rabbit / IgG |
| Tested Applications — Positive WB detected in | HeLa cells, HEK-293 cells, Jurkat cells, NIH/3T3 cells |
| Tested Applications — Positive IP detected in | HeLa cells |
| Tested Applications — Positive IHC detected in | human placenta tissue, mouse bladder tissue, mouse testis tissue, rat stomach tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Tested Applications — Positive FC (Intra) detected in | A431 cells |
| Recommended Dilutions — Western Blot (WB) | WB : 1:500-1:2000 |
| Recommended Dilutions — Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Recommended Dilutions — Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| Recommended Dilutions — Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
| Positive WB detected in | HeLa cells, HEK-293 cells, Jurkat cells, NIH/3T3 cells |
| Positive IP detected in | HeLa cells |
| Positive IHC detected in | human placenta tissue, mouse bladder tissue, mouse testis tissue, rat stomach tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive FC (Intra) detected in | A431 cells |
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | CatNo: Ag20748 Product name: Recombinant human SNRNP200 protein Source: e coli. -derived, PET30a Tag: 6*His Domain: 1787-2136 aa of BC001417 Sequence: ELVEQTLSDLEQSKCISIEDEMDVAPLNLGMIAAYYYINYTTIELFSMSLNAKTKVRGLIEIISNAAEYENIPIRHHEDNLLRQLAQKVPHKLNNPKFNDPHVKTNLLLQAHLSRMQLSAELQSDTEEILSKAIRLIQACVDVLSSNGWLSPALAAMELAQMVTQAMWSKDSYLKQLPHFTSEHIKRCTDKGVESVFDIMEMEDEERNALLQLTDSQIADVARFCNRYPNIELSYEVVDKDSIRSGGPVVVLVQLEREEEVTGPVIAPLFPQKREEGWWVVIGDAKSNSLISIKRLTLQQKAKVKLDFVAPATGAHNYTLYFMSDAYMGCDQEYKFSVDVKEAETDSDSD Predict reactive species |
| Full Name | small nuclear ribonucleoprotein 200kDa (U5) |
| Calculated Molecular Weight | 494 aa, 57 kDa |
| Observed Molecular Weight | 200-245 kDa |
| GenBank Accession Number | BC001417 |
| Gene Symbol | SNRNP200 |
| Gene ID (NCBI) | 23020 |
| RRID | AB_2879346 |
| Conjugate | Unconjugated |
| Purification Method | Antigen Affinity purified |
| UNIPROT ID | O75643 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA. |
| FC protocol for SNRNP200 antibody 23875-1-AP | Download protocol |
| IHC protocol for SNRNP200 antibody 23875-1-AP | Download protocol |
| IP protocol for SNRNP200 antibody 23875-1-AP | Download protocol |
| WB protocol for SNRNP200 antibody 23875-1-AP | Download protocol |
| human | WB,CoIP PLoS Biol CUL5 E3 ubiquitin ligase regulates the evasion of bladder cancer cells to CD8+ T cell-mediated killing by inhibiting autophagy. Authors - Xincheng Gao View Article |
| mouse | WB,RIP,CoIP Mol Ther Y4 RNA fragment mitigates myocardial ischemia-reperfusion injury in heart transplantation through SNRNP200-mediated enhancement of IL-10 pre-mRNA splicing Authors - Chuanghong Lu View Article |
| Roy (Verified Customer) (06-15-2024) | Works fine by WB to detect SNRNP200 from HeLa extracts (1/1000 dilution, overnight incubation at 4°C) Applications: Western Blot Primary Antibody Dilution: 1/1000 Cell Tissue Type: HeLa |
| Citations | 10 |
| Dilutions | WB : 1:500-1:2000 IP : 0.5-4.0 ug for IP and 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate for WB IHC : 1:500-1:2000 FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
| Isotype | IgG |
| Clonality | Polyclonal |
| Clone Number | - |
| Conjugation | Unconjugated |
SNRNP200 (small nuclear ribonucleoprotein 200kDa (U5)), also known as HELIC2, ASCC3L1 or BRR2, is a 2,136 amino acid protein that localizes to the nucleus and contains two SEC63 domains, two helicase C-terminal domains and two helicase ATP-binding domains. Existing as multiple alternatively spliced isoforms, HELIC2 functions as an RNA helicase that is thought to promote specific RNA-RNA conformational changes which are important in the second step of RNA splicing. The gene encoding HELIC2 maps to human chromosome 2, which houses over 1,400 genes and comprises nearly 8% of the human genome. Harlequin icthyosis, a rare and morbid skin deformity, is associated with mutations in the chromosome 2-localized ABCA12 gene, while the lipid metabolic disorder sitosterolemia is associated with defects in the ABCG5 and ABCG8 genes, hich also map to chromosome 2. The observed molecular weight of SNRNP200 is 200-245kd. Protocols Product Specific Protocols FC protocol for SNRNP200 antibody 23875-1-AP Download protocol IHC protocol for SNRNP200 antibody 23875-1-AP Download protocol IP protocol for SNRNP200 antibody 23875-1-AP Download protocol WB protocol for SNRNP200 antibody 23875-1-AP Download protocol Standard Protocols Click here to view our Standard Protocols
Publications
- RNA-coupled CRISPR screens reveal ZNF207 as a regulator of LMNA aberrant splicing in progeria.
Journal: Mol Cell | Authors: Amit K. Behera | Species: human | Application: WB - XAB2 depletion induces intron retention in POLR2A to impair global transcription and promote cellular senescence.
Journal: Nucleic Acids Res | Authors: Shuai Hou | Species: human | Application: WB - MEN1 is a regulator of alternative splicing and prevents R-loop-induced genome instability through suppression of RNA polymerase II elongation
Journal: Nucleic Acids Res | Authors: Bangming Jin | Species: human | Application: WB - Y4 RNA fragment mitigates myocardial ischemia-reperfusion injury in heart transplantation through SNRNP200-mediated enhancement of IL-10 pre-mRNA splicing
Journal: Mol Ther | Authors: Chuanghong Lu | Species: mouse | Application: WB,RIP,CoIP - SART3 reads methylarginine-marked glycine- and arginine-rich motifs
Journal: Cell Rep | Authors: Yalong Wang | Species: human | Application: WB - CUL5 E3 ubiquitin ligase regulates the evasion of bladder cancer cells to CD8+ T cell-mediated killing by inhibiting autophagy.
Journal: PLoS Biol | Authors: Xincheng Gao | Species: human | Application: WB,CoIP - KDM3A and KDM3B regulate alternative splicing in mouse pluripotent stem cells.
Journal: iScience | Authors: Caleb M. Dillingham | Species: mouse | Application: WB - Identification of a 35S U4/U6.U5 Tri-snRNP Complex Intermediate in Spliceosome Assembly.
Journal: J Biol Chem | Authors: Zhe Chen | Species: human | Application: IP - TREX2 component PCID2 scaffolds alternative SAC3-based subcomplexes with distinct RNA processing and export function.
Journal: bioRxiv | Authors: Vasilisa Aksenova | Species: human | Application: WB,IP,IF - RNA-coupled CRISPR Screens Reveal ZNF207 as a Regulator of LMNA Aberrant Splicing in Progeria
Journal: bioRxiv | Authors: Amit K Behera | Application: WB
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