| Catalog Number | 83857-6-RR |
| Synonyms | 240888B9, ALS1, EC:1.15.1.1, homodimer, hSod1 |
| Host | Rabbit |
| Reactivity | human, mouse and More (1) |
| Reactivity | human, mouse, bovine |
| Form | Liquid |
| Formulation | PBS, Azide, Glycerol |
| Applications | WB, IF-P, ELISA |
| Host / Isotype | Rabbit / IgG |
| Tested Applications — Positive WB detected in | HeLa cells, HEK-293 cells, K-562 cells |
| Tested Applications — Positive IF-P detected in | mouse brain tissue |
| Recommended Dilutions — Western Blot (WB) | WB : 1:5000-1:50000 |
| Recommended Dilutions — Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| Positive WB detected in | HeLa cells, HEK-293 cells, K-562 cells |
| Positive IF-P detected in | mouse brain tissue |
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| Tested Reactivity | human, mouse |
| Cited Reactivity | bovine |
| Class | Recombinant |
| Type | Antibody |
| Immunogen | CatNo: Eg1043 Product name: Recombinant Human SOD1 protein (His Tag) Source: mammalian cells -derived, pHZ-KIsec-C-6*HIS Tag: N-6*His Domain: 2-154 aa of NM_000454.5 Sequence: ATKAVCVLKGDGPVQGIINFEQKESNGPVKVWGSIKGLTEGLHGFHVHEFGDNTAGCTSAGPHFNPLSRKHGGPKDEERHVGDLGNVTADKDGVADVSIEDSVISLSGDHCIIGRTLVVHEKADDLGKGGNEESTKTGNAGSRLACGVIGIAQ Predict reactive species |
| Full Name | superoxide dismutase 1, soluble |
| Calculated Molecular Weight | 16kDa |
| Observed Molecular Weight | 16-20 kDa |
| GenBank Accession Number | NM_000454.5 |
| Gene Symbol | SOD1 |
| Gene ID (NCBI) | 6647 |
| RRID | AB_3743503 |
| Conjugate | Unconjugated |
| Purification Method | Protein A purification |
| UNIPROT ID | P00441 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA. |
| IF protocol for SOD1 antibody 83857-6-RR | Download protocol |
| WB protocol for SOD1 antibody 83857-6-RR | Download protocol |
| bovine | WB, IF Antioxidants (Basel) Development of an AAPH-Induced Oxidative Stress Model in Bovine Mammary Epithelial Cells and Investigation of Its Molecular Mechanisms. Authors - Yuanyuan Zhang View Article |
| Citations | 1 |
| Dilutions | WB : 1:5000-1:50000 IF-P : 1:50-1:500 |
| Isotype | IgG |
| Clonality | Recombinant |
| Clone Number | 240888B9 |
| Conjugation | Unconjugated |
Superoxide dismutase 1, soluble (amyotrophic lateral sclerosis 1 (adult)) (SOD1, synonyms: ALS, SOD, ALS1, IPOA) binds copper and zinc ions and is one of two isozymes responsible for destroying free uperoxide radicals in the body. This isozyme is a soluble cytoplasmic protein, acting as a homodimer to convert naturally-occuring but harmful superoxide radicals to molecular oxygen and hydrogen peroxide. The other isozyme is a mitochondrial protein. Mutations in this gene have been implicated as causes of familial amyotrophic lateral sclerosis. Protocols Product Specific Protocols IF protocol for SOD1 antibody 83857-6-RR Download protocol WB protocol for SOD1 antibody 83857-6-RR Download protocol Standard Protocols Click here to view our Standard Protocols
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