SPAST Monoclonal antibody 67361-1-Ig proteintech
$149.00
In stock
SKU
67361-1-Ig
| Catalog Number | 67361-1-Ig |
| Synonyms | 1F5A7, EC:5.6.1.1, spastin |
| Host | Mouse |
| Reactivity | human, mouse, rat |
| Form | Liquid |
| Formulation | PBS, Azide, Glycerol |
| Applications | WB, IHC, IF-P, ELISA |
| Host / Isotype | Mouse / IgG2b |
| Tested Applications — Positive WB detected in | HEK-293 cells, NCCIT cells, HeLa cells, PC-3 cells, HepG2 cells |
| Tested Applications — Positive IHC detected in | mouse brain tissue, human pancreas cancer tissue, human placenta tissue, mouse heart tissue, mouse pancreas tissue, rat brain tissue, rat heart tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Tested Applications — Positive IF-P detected in | mouse brain tissue |
| Recommended Dilutions — Western Blot (WB) | WB : 1:2000-1:10000 |
| Recommended Dilutions — Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| Recommended Dilutions — Immunofluorescence (IF)-P | IF-P : 1:200-1:800 |
| Positive WB detected in | HEK-293 cells, NCCIT cells, HeLa cells, PC-3 cells, HepG2 cells |
| Positive IHC detected in | mouse brain tissue, human pancreas cancer tissue, human placenta tissue, mouse heart tissue, mouse pancreas tissue, rat brain tissue, rat heart tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | mouse brain tissue |
| Western Blot (WB) | WB : 1:2000-1:10000 |
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| Immunofluorescence (IF)-P | IF-P : 1:200-1:800 |
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | CatNo: Ag18866 Product name: Recombinant human SPAST protein Source: e coli. -derived, PET28a Tag: 6*His Domain: 79-278 aa of BC150260 Sequence: CQRFSRALMAAKRSSGAAPAPASASAPAPVPGGEAERVRVFHKQAFEYISIALRIDEDEKAGQKEQAVEWYKKGIEELEKGIAVIVTGQGEQCERARRLQAKMMTNLVMAKDRLQLLESGAVPKRKDPLTHTSNSLPRSKTVMKTGSAGLSGHHRAPSYSGLSMVSGVKQGSGPAPTTHKGTPKTNRTNKPSTPTTATRK Predict reactive species |
| Full Name | spastin |
| Calculated Molecular Weight | 616 aa, 67 kDa |
| Observed Molecular Weight | 55 kDa |
| GenBank Accession Number | BC150260 |
| Gene Symbol | SPAST |
| Gene ID (NCBI) | 6683 |
| RRID | AB_2882613 |
| Conjugate | Unconjugated |
| Purification Method | Protein A purification |
| UNIPROT ID | Q9UBP0 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA. |
| IF protocol for SPAST antibody 67361-1-Ig | Download protocol |
| IHC protocol for SPAST antibody 67361-1-Ig | Download protocol |
| WB protocol for SPAST antibody 67361-1-Ig | Download protocol |
| human | WB Neurobiol Dis The ataxia-telangiectasia disease protein ATM controls vesicular protein secretion via CHGA and microtubule dynamics via CRMP5 Authors - Marina Reichlmeir View Article |
| Citations | 2 |
| Dilutions | WB : 1:2000-1:10000 IHC : 1:500-1:2000 IF-P : 1:200-1:800 |
| Isotype | IgG2b |
| Clonality | Monoclonal |
| Clone Number | 1F5A7 |
| Conjugation | Unconjugated |
SPAST encodes a microtubule-severing protein called spastin. By severing microtubules into small segments, SPAST participates in the regeneration of neurites. Mutations in the SPAST gene (previously known as SPG4) are the most common causes of hereditary spastic paraplegia (HSP-SPG4). Mammalian cells express at least two spastin isoforms, a less-abundant full-length form (M1-spastin, 68 kDa) and a more highly expressed isoform (M87-spastin, 60 kDa) that lacks the N-terminal 86 amino acids of the full-length protein. Also, there are two additional 64 kDa and 55 kDa isoforms due to alternative splicing of SPG4 exon 4. This antibody recognizes all these isoforms of spastin. Protocols Product Specific Protocols IF protocol for SPAST antibody 67361-1-Ig Download protocol IHC protocol for SPAST antibody 67361-1-Ig Download protocol WB protocol for SPAST antibody 67361-1-Ig Download protocol Standard Protocols Click here to view our Standard Protocols
Publications
- Reversing Autophagy Inhibition Ameliorates Neurodegeneration in Hereditary Spastic Paraplegia Caused by a Degradation-Resistant SPAST Mutation.
Journal: Mov Disord | Authors: Dingyi Zhang | Species: human | Application: WB,IF - The ataxia-telangiectasia disease protein ATM controls vesicular protein secretion via CHGA and microtubule dynamics via CRMP5
Journal: Neurobiol Dis | Authors: Marina Reichlmeir | Species: human | Application: WB
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