SPTA1 Polyclonal antibody 55227-1-AP proteintech
$149.00
In stock
SKU
55227-1-AP
| Catalog Number | 55227-1-AP |
| Synonyms | Alpha I spectrin, EL2 |
| Host | Rabbit |
| Reactivity | human, mouse, rat |
| Form | Liquid |
| Formulation | PBS, Azide, Glycerol |
| Applications | WB, IF-P, FC (Intra), ELISA |
| Host / Isotype | Rabbit / IgG |
| Tested Applications — Positive WB detected in | rat red blood cells, human blood |
| Tested Applications — Positive IF-P detected in | mouse lung tissue |
| Tested Applications — Positive FC (Intra) detected in | K-562 cells |
| Recommended Dilutions — Western Blot (WB) | WB : 1:200-1:1000 |
| Recommended Dilutions — Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| Recommended Dilutions — Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
| Positive WB detected in | rat red blood cells, human blood |
| Positive IF-P detected in | mouse lung tissue |
| Positive FC (Intra) detected in | K-562 cells |
| Western Blot (WB) | WB : 1:200-1:1000 |
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
| Tested Reactivity | human, mouse, rat |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Peptide Predict reactive species |
| Full Name | spectrin, alpha, erythrocytic 1 (elliptocytosis 2) |
| Calculated Molecular Weight | 280 kDa |
| Observed Molecular Weight | 280 kDa |
| GenBank Accession Number | NM_003126 |
| Gene Symbol | SPTA1 |
| Gene ID (NCBI) | 6708 |
| RRID | AB_3086436 |
| Conjugate | Unconjugated |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P02549 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA. |
| FC protocol for SPTA1 antibody 55227-1-AP | Download protocol |
| IF protocol for SPTA1 antibody 55227-1-AP | Download protocol |
| WB protocol for SPTA1 antibody 55227-1-AP | Download protocol |
| Citations | - |
| Dilutions | WB : 1:200-1:1000 IF-P : 1:50-1:500 FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
| Isotype | IgG |
| Clonality | Polyclonal |
| Clone Number | - |
| Conjugation | Unconjugated |
Alpha 1 Spectrin also known as SPTA1, EL2, belongs to the spectrin family. Spectrin is the major constituent of the cytoskeletal network underlying the erythrocyte plasma membrane. It associates with band 4.1 and actin to form the cytoskeletal superstructure of the erythrocyte plasma membrane. Mutations in SPTA1 result in a variety of hereditary red blood cell disorders, including elliptocytosis-2, pyropoikilocytosis, and spherocytosis, type 3(PMID: 1541680, 8941647). Protocols Product Specific Protocols FC protocol for SPTA1 antibody 55227-1-AP Download protocol IF protocol for SPTA1 antibody 55227-1-AP Download protocol WB protocol for SPTA1 antibody 55227-1-AP Download protocol Standard Protocols Click here to view our Standard Protocols
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